The growth dynamics of Lactobacillus acidophilus 145 and Lb. rhamnosus GG in substrate prepared from buckwheat, water or milk, sucrose or flavoring compound (vanilla and chocolate) was investigated. In general, during the fermentation (numbers at the end have reached values from 1.8?108 to 3.4?109 cfu g–1) or storage (Nfinal from 1.8?108 to 3.3?109 cfu g–1), the growth of individual strain was satisfactory. According to the evaluation of overall taste, the substrate with vanilla flavor and water, fermented by Lb. rhamnosus GG for 24 h and stored for 5 days, obtained the highest number of points. Lactobacilli had slightly cytotoxic effect on the HeLa cells and Caco-2 cells, and the percentage of inhibition after 48 and 72 h of exposure ranged from 40 to 96 % for both selected strains.

• MeSH
• apoptóza MeSH
• bezlepková dieta * MeSH
• Caco-2 buňky mikrobiologie   MeSH
• celiakie dietoterapie   MeSH
• Fagopyrum * MeSH
• fermentace MeSH
• HeLa buňky mikrobiologie   MeSH
• Lacticaseibacillus rhamnosus * izolace a purifikace   růst a vývoj   MeSH
• Lactobacillus acidophilus * izolace a purifikace   růst a vývoj   MeSH
• lidé MeSH
• probiotika * MeSH
• Check Tag
• lidé MeSH

Prebiotics are generally considered to promote the function or viability of probiotics via their fermentation, but their effect on the adherence of probiotics is still unclear. In this study, we examined the effect of 4 commercially available prebiotics [Orafti GR, Orafti P95, and Orafti Synergy (Beneo GmbH, Mannheim, Germany), and Vivinal (Friesland Foods Domo, Amersfoort, the Netherlands)] and 3 simple saccharides (glucose, galactose, and lactose) on the adherence of 5 probiotic type strains, 2 lactococci starter cultures, and 5 potential dairy probiotic strains from the Culture Collection of Dairy Microorganisms (Tábor, Czech Republic). Adherence was tested in microtiter plates on the following types of substrate: polystyrene alone and polystyrene coated with either porcine mucus or cocultures of the human colon cell lines Caco2 and HT29-MXT (1:9 ratio of HT29-MXT:Caco2). Adherence was evaluated as a change in fluorescence in the well of a microtiter plate. The most commonly observed effect (with a few exceptions) of prebiotics was decreased adherence of the tested strains observed on all types of substrate. The tested saccharides, which are part of the residual compounds of the used prebiotics, had a very similar effect-eliciting a decrease in adherence ability in the majority of the probiotic strains.

• MeSH
• Bifidobacterium izolace a purifikace   MeSH
• Caco-2 buňky MeSH
• Enterococcus izolace a purifikace   MeSH
• fermentace MeSH
• Lacticaseibacillus rhamnosus izolace a purifikace   MeSH
• Lactobacillus acidophilus izolace a purifikace   MeSH
• Lactococcus izolace a purifikace   MeSH
• lidé MeSH
• mléčné výrobky mikrobiologie   MeSH
• nádorové buněčné linie MeSH
• oligosacharidy aplikace a dávkování   MeSH
• potravinářská mikrobiologie MeSH
• prasata MeSH
• prebiotika * MeSH
• probiotika * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Německo MeSH
• Nizozemsko MeSH