- Klíčová slova
- granadaene,
- MeSH
- bakteriální zoonózy etiologie MeSH
- brouci mikrobiologie patogenita MeSH
- feces mikrobiologie MeSH
- grampozitivní koky * chemie izolace a purifikace patogenita MeSH
- jedlý hmyz * mikrobiologie patogenita MeSH
- Lactococcus chemie izolace a purifikace patogenita MeSH
- Leontopithecus mikrobiologie MeSH
- riziko MeSH
- střevní mikroflóra MeSH
- Publikační typ
- přehledy MeSH
Prebiotics are generally considered to promote the function or viability of probiotics via their fermentation, but their effect on the adherence of probiotics is still unclear. In this study, we examined the effect of 4 commercially available prebiotics [Orafti GR, Orafti P95, and Orafti Synergy (Beneo GmbH, Mannheim, Germany), and Vivinal (Friesland Foods Domo, Amersfoort, the Netherlands)] and 3 simple saccharides (glucose, galactose, and lactose) on the adherence of 5 probiotic type strains, 2 lactococci starter cultures, and 5 potential dairy probiotic strains from the Culture Collection of Dairy Microorganisms (Tábor, Czech Republic). Adherence was tested in microtiter plates on the following types of substrate: polystyrene alone and polystyrene coated with either porcine mucus or cocultures of the human colon cell lines Caco2 and HT29-MXT (1:9 ratio of HT29-MXT:Caco2). Adherence was evaluated as a change in fluorescence in the well of a microtiter plate. The most commonly observed effect (with a few exceptions) of prebiotics was decreased adherence of the tested strains observed on all types of substrate. The tested saccharides, which are part of the residual compounds of the used prebiotics, had a very similar effect-eliciting a decrease in adherence ability in the majority of the probiotic strains.
- MeSH
- Bifidobacterium izolace a purifikace MeSH
- Caco-2 buňky MeSH
- Enterococcus izolace a purifikace MeSH
- fermentace MeSH
- Lacticaseibacillus rhamnosus izolace a purifikace MeSH
- Lactobacillus acidophilus izolace a purifikace MeSH
- Lactococcus izolace a purifikace MeSH
- lidé MeSH
- mléčné výrobky mikrobiologie MeSH
- nádorové buněčné linie MeSH
- oligosacharidy aplikace a dávkování MeSH
- potravinářská mikrobiologie MeSH
- prasata MeSH
- prebiotika * MeSH
- probiotika * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Německo MeSH
- Nizozemsko MeSH
Early childhood caries (ECC) has become a serious medical problem worldwide in the last decade. Bacterial microflora of the dental plaque and oral cavity is considered an important factor in the formation and progression of dental caries. The aim of this study was strain typing and comparison of bacterial isolates retrieved from caries lesions and root canal contents of the same teeth. In total, 18 pairs of presumptive streptococci and lactobacilli retrieved from dental caries and root canals isolated from ECC-affected children, were selected on the basis of biotyping results and rep-PCR fingerprinting with (GTG)5 primer. Strain typing was further done using the RiboPrinter microbial characterization system (DuPont Qualicon). The automated ribotyping determined 14 pairs of the strains (77.8 %) to be identical. The results obtained confirmed that identical bacterial strains colonized both the decayed dental surface and the necrotic content of the dental pulp cavity during the cariogenesis. Our finding supports the assumption that bacteria could penetrate through the damaged dental surface to the inner parts of the teeth.
- MeSH
- dítě MeSH
- DNA fingerprinting MeSH
- kavita zubní dřeně mikrobiologie MeSH
- Lactococcus klasifikace genetika izolace a purifikace fyziologie MeSH
- lidé MeSH
- polymerázová řetězová reakce MeSH
- předškolní dítě MeSH
- ribotypizace MeSH
- Streptococcus klasifikace genetika izolace a purifikace fyziologie MeSH
- techniky typizace bakterií MeSH
- zubní kaz mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
