Apple mosaic virus (ApMV) is a widespread ssRNA virus which infects diverse species of Rosales. The phylogenetic analysis of complete capsid protein gene of the largest set of ApMV isolates discriminated two main clusters of isolates: one cluster correlates with Maloideae hosts and Trebouxia lichen algae hosts; a second with hop, Prunus, and other woody tree hosts. No correlation was found between clusters and geographic origin of virus isolates, and positive selection hypothesis in distinct hosts was not confirmed: in all virus populations, purifying selection had occurred. GGT→AAT substitution resulted in Gly→Asn change inside the zinc-finger motif in the capsid protein was revealed specific for discrimination of the clusters and we hypothesise that could influence the host preference.
- MeSH
- fylogeneze MeSH
- genetická variace * MeSH
- hostitelská specificita * MeSH
- Ilarvirus klasifikace genetika izolace a purifikace fyziologie MeSH
- Malus virologie MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- sekvence aminokyselin MeSH
- virové plášťové proteiny genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity. The tested compounds can be screened within 6-9 weeks in contrast to the 6 months for traditionally used explants on solid medium. The method enables large-scale screening of potential antivirals for in vitro elimination of viruses from vegetatively propagated crops and ornamentals.
- MeSH
- acyklické uhlovodíky farmakologie terapeutické užití MeSH
- antivirové látky farmakologie terapeutické užití MeSH
- Brassica účinky léků růst a vývoj virologie MeSH
- hydroponie metody MeSH
- kultivační média MeSH
- mikrobiální testy citlivosti metody MeSH
- nemoci rostlin terapie virologie MeSH
- nukleosidy farmakologie terapeutické užití MeSH
- replikace viru účinky léků MeSH
- ribavirin farmakologie terapeutické užití MeSH
- Tymovirus účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- DNA fingerprinting metody využití MeSH
- finanční podpora výzkumu jako téma MeSH
- grampozitivní bakterie imunologie klasifikace MeSH
- polymerázová řetězová reakce metody využití MeSH
- Publikační typ
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- izoelektrický bod MeSH
- kapsida analýza MeSH
- nemoci rostlin mikrobiologie MeSH
- protilátky virové MeSH
- viry mozaiky chemie izolace a purifikace ultrastruktura MeSH
- Publikační typ
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- Itálie MeSH
- MeSH
- chromatografie MeSH
- elektroforéza MeSH
- izoelektrický bod MeSH
- rostlinné viry MeSH
- Publikační typ
- techniky in vitro MeSH
