Marsh rosemary (Ledum palustre, Ericaceae) has been widely used in the traditional medicine of various regions worldwide, and as insect repellent. Little is known on its essential oil insecticidal potential. This study explored the insecticidal effects of the essential oil obtained from L. palustre growing in Poland on selected insect pests and vectors. GC-MS analysis evidenced an uncommon chemotype characterized by ascaridole (35.3% as sum of cis-ascaridole and isoascaridole) and p-cymene (25.5%). The essential oil was effective against Culex quinquefasciatus, Spodoptera littoralis and Musca domestica, showing LC50/LD50 of 66.6 mg L-1, 117.2 μg larva-1 and 61.4 μg adult-1, respectively. It was not toxic to non-target Eisenia fetida earthworms and moderately toxic to Daphnia magna microcrustaceans, over the positive control α-cypermethrin. The essential oil cytotoxicity on human keratinocytes and fibroblasts showed high IC50 values (71.3 and 84.4 μg mL-1, respectively). Comet assay data highlighted no DNA damages. Based on our findings, this essential oil, characterized by the ascaridole/p-cymene chemotype, could be a candidate for the formulation of botanical insecticides; large-scale production of green insecticides by this rare species may be assured by ex situ cultivation and biotechnological techniques.
- MeSH
- buněčné linie MeSH
- Culex účinky léků MeSH
- Culicidae účinky léků MeSH
- cymeny analýza farmakologie MeSH
- Daphnia účinky léků MeSH
- insekticidy analýza farmakologie MeSH
- keratinocyty cytologie účinky léků MeSH
- komáří přenašeči účinky léků MeSH
- kometový test MeSH
- larva účinky léků MeSH
- lidé MeSH
- monoterpeny s cyklohexanovým kruhem analýza farmakologie MeSH
- moucha domácí účinky léků MeSH
- můry účinky léků MeSH
- oleje prchavé analýza farmakologie MeSH
- Oligochaeta účinky léků MeSH
- peroxidy analýza farmakologie MeSH
- pyrethriny analýza farmakologie MeSH
- repelenty proti hmyzu analýza farmakologie MeSH
- rozmarýn chemie MeSH
- Spodoptera účinky léků MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Polsko MeSH
In the last decades, major research efforts have been done to investigate the insecticidal activity of plant-based products against mosquitoes. This is a modern and timely challenge in parasitology, aimed to reduce the frequent overuse of synthetic pesticides boosting resistance development in mosquitoes and causing serious threats to human health and environment. This review covers the huge amount of literature available on plant extracts tested as mosquito larvicides, particularly aqueous and alcoholic ones, due to their easy formulation in water without using surfactants. We analysed results obtained on more than 400 plant species, outlining that 29 of them have outstanding larvicidal activity (i.e., LC50 values below 10 ppm) against major vectors belonging to the genera Anopheles, Aedes and Culex, among others. Furthermore, synergistic and antagonistic effects between plant extracts and conventional pesticides, as well as among selected plant extracts are discussed. The efficacy of pure compounds isolated from the most effective plant extracts and - when available - their mechanism of action, as well as the impact on non-target species, is also covered. These belong to the following class of secondary metabolites: alkaloids, alkamides, sesquiterpenes, triterpenes, sterols, flavonoids, coumarins, anthraquinones, xanthones, acetogenonins and aliphatics. Their mode of action on mosquito larvae ranges from neurotoxic effects to inhibition of detoxificant enzymes and larval development and/or midugut damages. In the final section, current drawbacks as well as key challenges for future research, including technologies to synergize efficacy and improve stability - thus field performances - of the selected plant extracts, are outlined. Unfortunately, despite the huge amount of laboratory evidences about their efficacy, only a limited number of studies was aimed to validate their efficacy in the field, nor the epidemiological impact potentially arising from these vector control operations has been assessed. This strongly limits the development of commercial mosquito larvicides of botanical origin, at variance with plant-borne products developed in the latest decades to kill or repel other key arthropod species of medical and veterinary importance (e.g., ticks and lice), as well as mosquito adults. Further research on these issues is urgently needed.
- MeSH
- Culicidae účinky léků MeSH
- insekticidy farmakologie MeSH
- komáří přenašeči účinky léků MeSH
- larva účinky léků MeSH
- LD50 MeSH
- rostlinné extrakty farmakologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Persistence of Bacillus thuringiensis is an important factor in determining the success of this product as a pest control agent. In this report we present the development of a highly active mosquitocidal formulation with high resistance to UV. LLP29-M19 strain of Bt, selected by repeated exposure to UV was found to be highly resistant to UV. The product was optimized and the methods used were statistically analyzed. Using single-factor experiments it was determined that the optimal concentration of sodium alginate, CaCl2 and hollow glass beads in the formulation were 1.0%, 2.0% and 3.5%, respectively. Plackett-Burman design was used to screen the interaction of the three factors, CaCl2, sodium alginate and hollow glass beads in the sustained-release formulation. The best combined concentration and mutual effects of the three factors were optimized by response surface methodology. The results showed that the most favorable composition was sodium alginate 0.78%, CaCl2 4.52%, hollow glass bead 3.12%, bacterial powder 3.0%, melanin 0.015%, sodium benzoate 0.2%, and mouse feed 0.5%, resulting in the immobilization time of 4.5 h, at which time the corrected sustained-release virulence rose 2391.67 fold, which was 6.07-fold higher than the basic formulation and deviated only 5.0% from the value predicted by RSM.
- MeSH
- algináty farmakologie MeSH
- Bacillus thuringiensis fyziologie MeSH
- bakteriální proteiny farmakologie MeSH
- biologická kontrola škůdců metody MeSH
- biologická ochrana farmakologie MeSH
- chemie farmaceutická metody MeSH
- Culicidae účinky léků MeSH
- kyselina glukuronová farmakologie MeSH
- kyseliny hexuronové farmakologie MeSH
- larva účinky léků MeSH
- léky s prodlouženým účinkem farmakologie MeSH
- moskyti - kontrola metody MeSH
- ultrafialové záření MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- Culicidae účinky léků MeSH
- insekticidy MeSH
- Publikační typ
- srovnávací studie MeSH
