... Restriction fragment analysis 136 -- 11.1. Restriction endonucleases 137 -- 11.2. ... ... Optimal digestion conditions, 139 -- Method: Restriction digest of PCR products 140 -- 12. ... ... Generation of single-stranded DNA fragments 169 -- 14.2. ... ... labelling of oligonucleotides using photoactive biotin 236 -- Method 6: 3-end biotin labelling by terminal ... ... Pfu DNA polymerase (Pyrococcus furiosus) 250 -- Method: Amplification of a 365bp HIV-1-fragment using ...

... Prokaryotic DNA 53 Organization of Eukaryotic Chromatin 54 -- 2.5 SEQUENCE AND FUNCTION OF DNA 58 Restriction ... ... of Replication in Circular Genomes 150 Termination of Replication in Linear Genomes: -- Telomeres 150 ... ... ENDONUCLEASE, RESTRICTION MAPS, AND DNA SEQUENCING 258 -- Restriction Endonucleases Selectively Hydrolyze ... ... DNA 258 -- Restriction Maps Permit Routine Preparation of Defined Segments of DNA 259 -- Interrupted ... ... Enzymatic Cleavage Method: Sanger Procedure 260 -- Dye-Terminator Sequencing 262 -- RECOMBINANT DNA, ...

... Supercoiling influences the structure of the double helix 111 -- 6: Isolating the gene 115 -- A restriction ... ... map is constructed by cleaving DNA into specific fragments 117 -- Restriction sites can be used as genetic ... ... Sporulation utilizes a cascade of many sigma factors Racterial RNA polymerase has two modes of termination ... ... Licensing factor controls eukaryotic rereplication -- 16: Restriction and repair -- The consequences ... ... of modification and restriction -- Type II restriction enzymes are common -- The alternative activities ...

... Three Codons Terminate Protein Synthesis 172 -- ?? ... ... Bacterial RNA Polymerase Terminates at Discrete Sites 286 -- ??? ... ... Antitermination Requires Sites That Are Independent of the Terminators 292 iii? ... ... M Termination and Antitermination Factors Interact with RNA Polymerase 293 -- ??? ... ... Okazaki Fragments Are Linked by Ligase 443 -- ?? ...

... That Are Specified by Genes S3 -- 3.2.2 Polypeptide Chains Are Flexible Yet -- Conformationally Restricted ... ... Enzymes Split DNA into Specific -- Fragments 144 -- 6.1.2 Restriction Fragments Can Be Separated by ... ... Gel -- Electrophoresis and Visualized 145 -- 6.1.3 DNA Is Usually Sequenced by Controlled -- Termination ... ... 4 Cleavage Is by In-Line Displacement of 3\' Oxygen from PbnsnhnrilQ im - A YX/otor -- 9.3.2 Restriction ... ... by Aldolase 431 -- 16.1.4 Triose Phosphate Isomerase Salvages a -- Three-Carbon Fragment 431 -- 16.1.5 ...

... RNA Polymerase: A Complex Enzyme 302 Initiation of RNA Chains 303 Elongation of RNA Chains 303 Termination ... ... Initiation of RNA Chains 307 RNA Chain Elongation and the Addition of 5\' Methyl Guanosine Caps 308 Termination ... ... Dwarfism with Human Growth Hormone 517 Basic Techniques Used to Clone Genes 518 -- The Discovery of Restriction ... ... Genomics: An Overview 555 Correlated Genetic, Cytological, and Physical Maps of Chromosomes 555 Restriction ... ... Fragment-Length Polymorphism (RFLP) and Microsatellite Maps 558 Cytogenetic Maps 562 Physical Maps and ...

... Enzymes and DNA Ligases Allow -- Insertion of DNA Fragments into Cloning Vectors 176 -- E. coli Plasmid ... ... Vectors Are Suitable for Cloning -- Isolated DNA Fragments 178 cDNA Libraries Represent the Sequences ... ... 184 -- Cloned DNA Molecules Are Sequenced Rapidly by the Dideoxy Chain-Termination Method 187 -- The ... ... to Study Gene Expression 191 -- Hybridization Techniques Permit Detection of Specific DNA Fragments ... ... -- Cells 879 -- Mammalian Restriction Point Is Analogous to START in Yeast Cells 880 -- Multiple CDKs ...

... (Basel): Isolation of Distinct T Helper Cell Popula tions with the Same Hapten-Specificity but Restricted ... ... Marseille): Receptors for Self Major Histocompatibility Complex (MHC) Antigens on Alloreactive and MHC-Restricted ... ... (Cincinnati, Ohio): Modulation of Immune Response by Antigenic Fragments -- Hartmann, K. ... ... (Geneva/Basel): The Phosphorylcholine Reper toire of BALB/c Mice as Studied by N-Terminal Amino Acid ...

... structures, whereas the T-cell receptor is specialized to recognize foreign antigens as peptide fragments ... ... multicellular organisms. 61 -- 2-12 Surface-bound C3 convertase deposits large numbers of -- C3b fragments ... ... of some complement proteins initiate a local inflammatory response. 64 -- 2-15 The terminal complement ... ... 138 -- Antigen recognition by T cells. 138 -- 4-10 The T-cell receptor is very similar to a Fab fragment ... ... 6-9 Cross-presentation allows exogenous proteins to be presented on MHC class I molecules by a restricted ...