JC and BK polyomaviruses (JCV and BKV) infect humans and can cause severe illnesses in immunocompromised patients. Merkel cell polyomavirus (MCPyV) can be found in skin carcinomas. In this study, we assessed the occurrence of serum antibodies against MCPyV, BKV, and JCV polyomaviruses in a healthy population of the Czech Republic. Serum samples from 991 healthy individuals (age range: 6-64 years) were examined by enzyme-linked immunoassay (ELISA) using virus-like particles (VLPs) based on the major VP1 capsid proteins of these viruses. Overall, serum antibodies against MCPyV, JCV, and BKV were found in 63%, 57%, and 69%, respectively, of this population. For all three viruses, these rates were associated with age; the occurrence of antibodies against MCPyV and JCV was highest for those older than 59 years, while the occurrence of antibodies against BKV was highest in those aged 10-19 years and 20-29 years. This is the first large study to determine the seroprevalence rates for BKV, JCV, and MCPyV polyomaviruses in the general Czech Republic population.

• Klíčová slova
• antibodies, epidemiology, polyomavirus, prevalence,
• MeSH
• buněčné linie MeSH
• dítě MeSH
• dospělí MeSH
• infekce onkogenními viry krev   epidemiologie   virologie   MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• Merkelův polyomavirus imunologie   MeSH
• mladiství MeSH
• mladý dospělý MeSH
• myši inbrední ICR MeSH
• polyomavirové infekce krev   epidemiologie   virologie   MeSH
• předškolní dítě MeSH
• protilátky virové krev   MeSH
• séroepidemiologické studie MeSH
• studie případů a kontrol MeSH
• věkové rozložení MeSH
• virus BK imunologie   MeSH
• virus JC imunologie   MeSH
• zvířata MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH
• Názvy látek
• protilátky virové MeSH

Novel biomaterials based on hydrophilic polycaprolactone and polyurethane (Tecophilic®) nanofibers with an encapsulated 5,10,5,20-tetraphenylporphyrin photosensitizer were prepared by electrospinning. The doped nanofiber textiles efficiently photo-generate O(2)((1)Δ(g)), which oxidize external chemical and biological substrates/targets. Strong photo-virucidal effects toward non-enveloped polyomaviruses and enveloped baculoviruses were observed on the surface of these textiles. The photo-virucidal effect was confirmed by a decrease in virus infectivity. In contrast, no virucidal effect was detected in the absence of light and/or the encapsulated photosensitizer.

• MeSH
• anthraceny chemie   MeSH
• antivirové látky farmakologie   MeSH
• Baculoviridae účinky léků   MeSH
• fotosenzibilizující látky farmakologie   MeSH
• inaktivace viru účinky léků   MeSH
• kapsida chemie   MeSH
• myši MeSH
• nanovlákna chemie   ultrastruktura   MeSH
• oxidace-redukce MeSH
• polyestery chemie   MeSH
• Polyomavirus účinky léků   MeSH
• polyurethany chemie   MeSH
• porfyriny farmakologie   MeSH
• rekombinace genetická genetika   MeSH
• singletový kyslík metabolismus   MeSH
• textilie * MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anthraceny MeSH
• antivirové látky MeSH
• fotosenzibilizující látky MeSH
• polycaprolactone MeSH Prohlížeč
• polyestery MeSH
• polyurethany MeSH
• porfyriny MeSH
• singletový kyslík MeSH
• tetraphenylporphyrin MeSH Prohlížeč

Mouse polyomavirus-like particles (MPyV-VLPs) carrying inside a fragment of the Bcr-Abl hybrid protein containing the epitope of chronic myeloid leukemia fusion region were prepared. A sequence encoding 171 amino acids covering Bcr-Abl breakpoint was fused to the C-terminal part of VP3 minor protein connecting it to the VP1 capsomeres. Chimeric particles, the Bcr-Abl VLPs, were tested for their ability to induce Bcr-Abl specific immune response in mice after their intranasal (i.n.) or intraperitoneal (i.p.) administration without any other adjuvants. Bcr-Abl VLPs induced strong anti-VP1 immune response in both i.n. and i.p. immunized mice. As expected, neither IgG nor IgM anti-Bcr-Abl specific antibodies were detected in the sera of immunized animals. Surprisingly, no specific CTL (cytotoxic T-lymphocyte) activity was proved using two different methods (in vitro cytotoxicity assay with CFSE-labeled target cells and highly sensitive cytotoxicity assay using MHC class I Bcr-Abl specific pentamers). In addition, no proliferative response of T-cells of i.n. immunized mice after in vitro restimulation with antigen-pulsed bone marrow-derived dendritic cells was observed. Taken together, Bcr-Abl breakpoint epitopes appeared to be weak immunogens and even MPyV-VLPs did not provide sufficient adjuvant ability to support induction of immune responses specific to Bcr-Abl fusion zone epitope.

• MeSH
• antigeny virové imunologie   MeSH
• bcr-abl fúzní proteiny imunologie   MeSH
• chronická myeloidní leukemie imunologie   MeSH
• cytotoxicita imunologická MeSH
• epitopy imunologie   MeSH
• fluorescenční protilátková technika MeSH
• imunoelektronová mikroskopie MeSH
• lidé MeSH
• myši MeSH
• Polyomavirus imunologie   MeSH
• rekombinantní proteiny imunologie   MeSH
• western blotting MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny virové MeSH
• bcr-abl fúzní proteiny MeSH
• epitopy MeSH
• rekombinantní proteiny MeSH

Dogs represent both an important veterinary species and a convenient model for allogeneic hematopoietic stem cell transplantation. Even though anti-canine CD34 antibodies have recently become available, little is known about hematopoietic lineages in dogs, partially because CD34- cells have been ignored in all analyses performed so far. In this study, we have focused on the bone marrow mononuclear compartment to provide an additional piece of information on the phenotype of CD34+ progenitors and to identify the dominant CD34- population. We have shown that, in contrast to the adults, mature lymphocytes are scarce in neonatal dog bone marrow. Using cross-reactive antibodies against CD79alpha we have shown that the B lineage of hematopoiesis strongly prevails. CD34+ cells were shown to be positive for MHC class II and SWC3, a member of the signal regulatory protein family.

• MeSH
• antigeny CD34 imunologie   MeSH
• antigeny CD79 MeSH
• CD antigeny imunologie   MeSH
• hematopoetické kmenové buňky imunologie   MeSH
• leukocyty imunologie   MeSH
• novorozená zvířata MeSH
• průtoková cytometrie veterinární   MeSH
• psi imunologie   MeSH
• receptory antigenů B-buněk imunologie   MeSH
• zvířata MeSH
• Check Tag
• psi imunologie   MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny CD34 MeSH
• antigeny CD79 MeSH
• CD antigeny MeSH
• receptory antigenů B-buněk MeSH