Natural products have many healing effects on the skin with minimal or no adverse effects. In this study, we analyzed the regenerative properties of a waste product (hydrolate) derived from Helichrysum italicum (HH) on scratch-tested skin cell populations seeded on a fluidic culture system. Helichrysum italicum has always been recognized in the traditional medicine of Mediterranean countries for its wide pharmacological activities. We recreated skin physiology with a bioreactor that mimics skin stem cell (SSCs) and fibroblast (HFF1) communication as in vivo skin layers. Dynamic culture models represent an essential instrument for recreating and preserving the complex multicellular organization and interactions of the cellular microenvironment. Both cell types were exposed to two different concentrations of HH after the scratch assay and were compared to untreated control cells. Collagen is the constituent of many wound care products that act directly on the damaged wound environment. We analyzed the role played by HH in stimulating collagen production during tissue repair, both in static and dynamic culture conditions, by a confocal microscopic analysis. In addition, we performed a gene expression analysis that revealed the activation of a molecular program of stemness in treated skin stem cells. Altogether, our results indicate a future translational application of this natural extract to support skin regeneration and define a new protocol to recreate a dynamic process of healing.
- Klíčová slova
- Helichrysum italicum, bioreactor, dynamic cultures, fibroblasts, molecular mechanisms, stem cells, tissue regeneration, wound healing,
- MeSH
- fibroblasty metabolismus účinky léků MeSH
- Helichrysum * chemie MeSH
- hojení ran * účinky léků MeSH
- kmenové buňky metabolismus účinky léků cytologie MeSH
- kolagen * metabolismus MeSH
- kultivované buňky MeSH
- kůže * metabolismus účinky léků MeSH
- lidé MeSH
- regenerace * účinky léků MeSH
- rostlinné extrakty * farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- kolagen * MeSH
- rostlinné extrakty * MeSH
Wound healing is a dynamic process involving different cell types with distinct roles according to the stages of healing. Fibroblasts and stem cells actively participate in tissue regeneration. A proper stimulation could contribute to enhance wound healing process-es. Helichrysum italicum (H. italicum) is a medical plant well described for its pharmacological, antimicrobial, and anti-inflammatory activities. Aim of the present work was to examine the effect of the hydrolat derivate from H. italicum on stem cells isolated from skin and fibroblasts in vitro in presence or absence of tissue damage. The viability and proliferation of all cell types cultured in dif-ferent conditions were analyzed by MTT and BrdU assays. Cell proliferation after wound was analyzed with scratch test. Also, the expression of the main genes involved in tissue repair was evaluated by RT-qPCR analysis. Here we describe the capability of hy-drolat of H. italicum to promote tissue regeneration after scratch test both in stem cells and in fibroblasts. Moreover, the gene ex-pression analysis revealed that, hydrolat of H. italicum is also able to enhance stemness related. In conclusion our results are en-couraging, highlighting novel regenerative properties of hydrolat of H. italicum and paving the way for future application of this wasting product in accelerating wound healing.
- MeSH
- antiflogistika farmakologie MeSH
- fibroblasty metabolismus MeSH
- Helichrysum * MeSH
- hojení ran MeSH
- kmenové buňky MeSH
- kůže MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antiflogistika MeSH
The phytochemical analysis of a methanolic extract from Helichrysum petiolare Hilliard & B. L. (Asteraceae) confirmed the content of phenylpropanoids and flavonoids. Five secondary metabolites were isolated using preparative HPLC, namely coumarin scopolin (1), 3-chlorogenic acid (2), caffeic acid-hexose derivative (3), dicaffeoylquinic acid (5), and the flavonoid isoquercitrin (4). These compounds were identified from this species for the first time. Only dicaffeoylquinic acid was able to inhibit Escherichia coli CCM 7929 at the concertation of 512 μg mL-1 in a screening of antibacterial activity.
- Klíčová slova
- HPLC, Helichrysum petiolare, antibacterial activity, plant phenols,
- MeSH
- antioxidancia farmakologie MeSH
- Asteraceae * MeSH
- flavonoidy farmakologie MeSH
- fytonutrienty chemie MeSH
- Helichrysum * chemie MeSH
- rostlinné extrakty analýza MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antioxidancia MeSH
- flavonoidy MeSH
- fytonutrienty MeSH
- rostlinné extrakty MeSH
Helichrysum faradifani (Asteraceae) is a perennial shrub growing in rocky and sandy places of Madagascar. The plant is used in the Malagasy traditional medicine as a wound-healing agent, disinfectant and for the treatment of syphilis, diarrhea, cough and headache. In the present work, we analysed the chemical composition of the essential oil distilled from the aerial parts of H. faradifani by GC-MS and evaluated its insecticidal activity against 2nd, 3rd and 4th instar larvae of the lymphatic filariasis vector Culex quinquefasciatus by acute toxicity assays. The most sensitive were 2nd instar (LC50 = 85.7 μL L-1) larvae. For the 3rd and 4th instar larvae, the estimated LC50 were 156.8 and 134.1 μL L-1, respectively. Monoterpene hydrocarbons (51.6%) were the major fraction of the essential oil, with the bicyclic α-fenchene (35.6%) as the predominant component. Sesquiterpene hydrocarbons (34.0%) were the second major group characterising the oil, with γ-curcumene (17.7%) as the most abundant component.
- Klíčová slova
- Culex quinquefasciatus, GC-MS, Helichrysum faradifani, St. Louis encephalitis; essential oil, insecticidal activity,
- MeSH
- Culex účinky léků MeSH
- Helichrysum chemie MeSH
- insekticidy chemie farmakologie MeSH
- komáří přenašeči účinky léků MeSH
- larva účinky léků MeSH
- monoterpeny analýza chemie MeSH
- nadzemní části rostlin chemie MeSH
- oleje prchavé chemie farmakologie MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- rostlinné extrakty chemie MeSH
- seskviterpeny analýza chemie MeSH
- testy akutní toxicity MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Madagaskar MeSH
- Názvy látek
- insekticidy MeSH
- monoterpeny MeSH
- oleje prchavé MeSH
- rostlinné extrakty MeSH
- seskviterpeny MeSH
Helichrysum italicum (Roth) G. Don is a flowering plant of the family Asteraceae. It is rich in oil that is used for different medicinal purposes and in fragrance industry. Volatile profile of four populations of H. italicum, collected from natural habitat in Dalmatia (Croatia), was analysed by capillary GC-MS. Sample from BraČ Island had α-trans-bergamotene (10.2%) and β-acoradiene (10.1%) as the majors, whereas sample collected on Biokovo Mt. was rich in neryl acetate (8.1%). β-Acoradiene was also the main constituent of sample collected near Tijarica, whereas rosifoliol (8.5%) was the most abundant constituent in sample collected near Makarska. Presented results show the influence of environmental conditions on chemical differentiation of the volatiles of H. italicum from Croatia.
- Klíčová slova
- GC–MS, Helichrysum italicum, essential oil,
- MeSH
- Helichrysum chemie MeSH
- můstkové bicyklické sloučeniny analýza MeSH
- nadzemní části rostlin chemie MeSH
- oleje prchavé analýza MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- seskviterpeny analýza MeSH
- životní prostředí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Chorvatsko MeSH
- Názvy látek
- alpha-bergamotene MeSH Prohlížeč
- můstkové bicyklické sloučeniny MeSH
- oleje prchavé MeSH
- seskviterpeny MeSH
The effect of combinations of the crude acetone and aqueous extracts of Helichrysum pedunculatum leaves and eight antibiotics was determined by means of checkerboard and time-kill methods. In the checkerboard method, synergy of 45.8% was observed, being independent of Gram reaction, with combinations in the aqueous extract yielding largely (18.8%) antagonistic interactions. The time-kill assay detected synergy (45.8%) that was also independent of Gram reaction with a potentiation of more than 3 orders of the bactericidal activity of the test antibiotics. The crude leaf extracts of H. pedunculatum could thus be considered to be potential source of a broad-spectrum antibiotic-resistance-modifying compounds.
- MeSH
- antibakteriální látky farmakologie MeSH
- Bacteria účinky léků MeSH
- Helichrysum chemie MeSH
- infekce v ráně farmakoterapie mikrobiologie MeSH
- kombinovaná farmakoterapie MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- rostlinné extrakty farmakologie MeSH
- synergismus léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- rostlinné extrakty MeSH
- Klíčová slova
- FLAVONES/pharmacology *, LIVER DISEASES/experimental *, PLANTS, MEDICINAL/pharmacology *,
- MeSH
- flavony farmakologie MeSH
- Helichrysum * MeSH
- léčivé rostliny farmakologie MeSH
- lidé MeSH
- nemoci jater * MeSH
- rostliny * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- flavone MeSH Prohlížeč
- flavony MeSH
