Although the microscopic examination of stool samples remains the reference method of choice for the diagnosis of intestinal protistan infections, this method is time-consuming and requires experienced and well-trained operators. The purpose of this study was to evaluate the level of agreement between the BD MAX TM Enteric Parasite Panel (EPP) and microscopy for the detection of Giardia intestinalis (Lambl, 1859), Cryptosporidium spp. and Entamoeba histolytica Schaudinn, 1903 in stool samples. The study included faecal samples of 362 patients who were admitted to our hospital due to gastrointestinal complaints. In the microscopic examination, which was made with the native-lugol method on the stool samples that were taken from the patients, cysts, trophozoites and eggs of the parasite were examined. The diagnosis of G. intestinalis, Cryptosporidium parvum Tyzzer, 1912 and Cryptosporidium hominis Morgan-Ryan, Fall, Ward, Hijjawi, Sulaiman, Fayer, Thompson, Olson, Lal et Xiao, 2002, and E. histolytica was made in the faecal samples using the EPP assay. In the microscopic examination, Cryptosporidium spp. positive stool samples were stained with kinyoun's acid-fast. In the microscopic examination, parasites were detected in 41 (11%) of the 362 stool samples. In contrast, EPP assay identified parasites in 23 (6.3%) of the samples. In the microscopic examination, E. histolytica and Entamoeba dispar Brumpt, 1925 were detected in 22 (6.1%) of the samples, G. intestinalis was seen in 15 (4.1%), and C. parvum or C. hominis were detected in three (0.8%); these values were five (1.4%), 16 (4.4%) and two (0.5%) positive with the EPP assay. Although C. parvum or C. hominis were detected as positive in the microscopic examination of three samples, only two of the samples were positive in both EPP assay and kinyoun's acid-fast method. The EPP assay is a relatively simple test that can distinguish E. histolytica and E. dispar, but it cannot replace microscopy in the diagnosis of amoebiasis. Diagnosis for G. intestinalis and C. parvum/C. hominis with the BD MAXTM enteric parasite panel was equivalent to that with microscopy. We believe that E. histolytica must be diagnosed with nucleic acid amplification tests that have a high sensitivity and specificity like EPP assay in certain patient groups.

• Klíčová slova
• Cryptosporidium spp., enteric parasites, stool microscopy,
• MeSH
• Cryptosporidium parvum izolace a purifikace   MeSH
• Cryptosporidium izolace a purifikace   MeSH
• dospělí MeSH
• entamébóza epidemiologie   parazitologie   MeSH
• Entamoeba histolytica izolace a purifikace   MeSH
• feces parazitologie   MeSH
• Giardia lamblia izolace a purifikace   MeSH
• giardiáza epidemiologie   parazitologie   MeSH
• kryptosporidióza epidemiologie   parazitologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• prevalence MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Turecko epidemiologie   MeSH

BACKGROUND: In the Czech Republic, 40 to 50 cases of Entamoeba histolytica/dispar infections are reported annually to the National Reference Laboratory for diagnostics of intestinal parasitoses. However, the actual number of patients with Entamoeba histolytica infection is unknown as diagnosis relies on cyst detection in faecal samples by microscopy, the method which cannot differentiate between pathogenic E. histolytica and nonpathogenic E. dispar. The aim of this study was (1) to evaluate the proportions between E.histolytica and E. dispar in patients, mainly travellers, using multiplex nested PCR technique, (2) to evaluate specificity of the technique for detection of these species. METHODS AND RESULTS: Faecal samples from 68 patients microscopically positive for cysts of E. histolytica/dispar were tested by PCR. Of these, 65 persons (95.6%) were positive for E. dispar, whereas only 3 patients (4.4%) were positive for E. histolytica. CONCLUSIONS: This study shows that the number of patients infected with pathogenic E. histolytica is very low in the Czech Republic and points to the necessity of differentiation of Entamoeba species for physician's decision in treatment of E. histolvtica- or E.dispar-infected persons.

• MeSH
• amébová dyzenterie diagnóza   parazitologie   MeSH
• cestování MeSH
• Entamoeba histolytica izolace a purifikace   MeSH
• Entamoeba izolace a purifikace   MeSH
• feces parazitologie   MeSH
• lidé MeSH
• polymerázová řetězová reakce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

Two pilot studies on malaria, leishmaniasis, schistosomiasis and intestinal parasites were carried out in 104 children 6-15 years old from villages in the lowland and highland areas of South Yemen in November 1988. Some of the results presented are in the order lowland and highland. The occurrence of malaria parasites in blood smears was 6.7% and 3.8%. P. falciparum was detected only. The antimalarial IFAT antibodies were proved in 66.7% and 11.5% respectively. IFAT antibodies against leishmania were in 43.8% and 39.3% respectively of sera examined. Ova of S. haematobium diagnosed with the frequency 29.0% and 13.3% respectively. S. mansoni infection was found in 19.2% of examined children in highland only. From parasites found in stool specimens should be mentioned E. histolytica (42.3% and 36.8%), G. lamblia (34.6% and 35.1%). The other intestinal protozoa were less frequent. Further details of sex and age groups distribution are mentioned.

• MeSH
• dítě MeSH
• Entamoeba histolytica izolace a purifikace   MeSH
• feces parazitologie   MeSH
• Giardia lamblia izolace a purifikace   MeSH
• leishmanióza krev   epidemiologie   MeSH
• lidé MeSH
• mladiství MeSH
• pilotní projekty MeSH
• prevalence MeSH
• schistosomiasis haematobia epidemiologie   moč   MeSH
• schistosomiasis mansoni epidemiologie   moč   MeSH
• tropická malárie krev   epidemiologie   MeSH
• zvířata MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Jemen epidemiologie   MeSH

A total of 2,883 foreign students at the age of 18-30 years were examined for amoebiasis after their arrival to Czechoslovakia. Stool examinations revealed the presence of Entamoeba histolytica in 112 of them (3.9%). Students from 38 countries were found to be infected with this parasite. In a set of 2,064 students from these countries E. histolytica prevalence in stool was 5.4%. There were greater differences in the prevalence between individual countries inside a geographical region than between individual geographical regions. The highest E. histolytica prevalence in stool was found in students from tropical and southern Africa (6.7% of 745 examined) and the lowest in students from South-eastern Asia (3.1% of 321 examined). In a simple cross-section study, antibodies against E. histolytica were detected by enzyme-linked immunosorbent assay (ELISA) in the sera of 1,001 persons. Antibodies were detected in 7.9% of students at the following titres: 1:200 in 4.5%, 1:600 in 1.5%, 1:1,800 in 1.9%. Antibodies occurred more frequently in students carrying E. histolytica cysts (X2 = 14.9). Titre of ELISA antibodies in patients with confirmed liver abscess was higher than 1:1,800. counterimmunoelectrophoresis (CIEP) test was used for serum examinations of patients who had been demonstrated by ELISA to be seropositive and of those carrying E. histolytica cysts. In a set of 170 patients CIEP antibodies were also more frequent in those carrying E. histolytica cysts (X2 = 26.95). A comparison of the results of ELISA and CIEP tests in the same patients revealed that CIEP antibodies were more dependent on the actual parasitization with E. histolytica than ELISA antibodies.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• amébiáza epidemiologie   MeSH
• cestování MeSH
• dospělí MeSH
• ELISA MeSH
• entamébóza epidemiologie   etnologie   MeSH
• Entamoeba histolytica imunologie   izolace a purifikace   MeSH
• feces parazitologie   MeSH
• imunoelektroforéza protisměrná MeSH
• lidé MeSH
• prevalence MeSH
• protilátky protozoální analýza   MeSH
• průřezové studie MeSH
• studenti MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Afrika etnologie   MeSH
• Asie etnologie   MeSH
• Československo epidemiologie   MeSH
• jihovýchodní Asie etnologie   MeSH
• Latinská Amerika etnologie   MeSH
• Střední východ etnologie   MeSH
• Názvy látek
• protilátky protozoální MeSH

Among 72 patients clinically suspected of Entamoeba histolytica (E. histolytica) infections, 39 positive cases (54%) were detected serologically by the indirect hemagglutination (IHA) test. Parasitologically, microscopic examination of three consecutive stool specimens from all these patients indicated positivity for E. histolytica cysts and or trophozoites in 10 of the patients with IHA antibody titers greater than or equal to 1:128, which is of clinical significance. Another 2 patients were parasitologically positive but showed low IHA antibody titres (1:32-1:64); follow up indicated response to treatment with metronidazole. The highest serological positivity (100%) were detected in patients with liver abscess, all were clinically proven cases of extra-intestinal amoebiasis. IHA antibody levels of clinical significance were seen in all four patients with chronic dysentery with parasitological evidence of E. histolytica in their stools. In a group of patients with abdominal pain nine positives were detected serologically, four of which were positively diagnosed concurrently by parasitology; the remaining five patient's sera showed high IHA antibody titres with absence of cysts or trophozoites in stools, indicative possibly of persistence of antibodies from past infection. The serologic determination of E. histolytica IHA antibodies in a control group consisting of normal healthy school children and adults of both sexes without any clinical evidence of amoebiasis showed the absence of any positive titres of clinical significance; low titres (1:32-1:64) were detected in 5.2% of 232 sera tested. Parasitological examination of three consecutive stool specimens from all individuals in the control group showed the presence of cysts of E. histolytica in just two among 232 tested (0.9%).

• MeSH
• amébiáza diagnóza   MeSH
• dítě MeSH
• dospělí MeSH
• entamébóza diagnóza   MeSH
• Entamoeba histolytica izolace a purifikace   MeSH
• feces parazitologie   MeSH
• hemaglutinační testy MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• protilátky protozoální analýza   MeSH
• zvířata MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky protozoální MeSH

Immunisation of hamsters with the Sephadex G-200 chromatographed Fraction-I of the crude amoebic extract afforded protection in 58% of the animals against an intrahepatic challenge with a virulent subline of axenic Entamoeba histolytica NIH-200 (V). The protected animals had variable levels of anti-FI antiamoebic antibodies, while none of the infected controls had such antibodies. Findings suggest that chromatographed FI of crude amoebic extract might function as a potent immunogen affording protection in amoebic infection.

• MeSH
• amébový absces jater imunologie   parazitologie   MeSH
• antigeny protozoální imunologie   MeSH
• Entamoeba histolytica imunologie   izolace a purifikace   MeSH
• hemaglutinační testy MeSH
• imunizace MeSH
• játra parazitologie   MeSH
• křečci praví MeSH
• křeček rodu Mesocricetus MeSH
• tvorba protilátek MeSH
• zvířata MeSH
• Check Tag
• křečci praví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antigeny protozoální MeSH

Results of histopathological examination of a solitary chronical amoebic abscess in the liver of a patient from Cambodia are described. The abscess was at a proliferously reparative phase and Entamoeba histolytica was detected at the border between the necrosis and inner wall of abscess, as well as in the proper non-specific granulation tissue of the inner layers of abscess capsule. A differential diagnosis of the amoebic abscess from similar parasitary or pseudoparasitary liver lesions and differential diagnosis of amoebae in histological sections are given. For orientation examinations of the liver tissue for the presence of amoebae the authors recommend the impregnation after Grocott and staining with Goldner's trichrome for a more detailed evaluation of histological sections.

• MeSH
• amébový absces jater diagnóza   parazitologie   patologie   MeSH
• barvení a značení MeSH
• Entamoeba histolytica izolace a purifikace   parazitologie   MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Geografické názvy
• Kambodža MeSH

• MeSH
• amébová dyzenterie diagnóza   MeSH
• Entamoeba histolytica růst a vývoj   izolace a purifikace   MeSH
• kultivační média MeSH
• lidé MeSH
• parazitární nemoci střev diagnóza   MeSH
• parazitologie metody   MeSH
• střeva parazitologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• kultivační média MeSH