Until now, few cases of coincidental giardiasis and pancreatic tumors have been described. Among these cases, three described giardiasis cases coincided with confirmed pancreatic cancer. We present another case of Giardia infection coexisting with pancreatic cancer in a 67-year-old man who suffered from stenosis of the distal ductus choledochus combined with a hypoechoic mass in the head of the pancreas. The diagnostic conclusion of suspicious adenocarcinoma was based on endoscopic ultrasound fine-needle aspiration (EUS-FNA) biopsy and confirmed by a partial duodenopancreatectomy. On bloody cytology smears prepared from the EUS-FNA specimen, trophozoites of Giardia intestinalis accompanying an inflammatory background and features that fulfilled the morphological criteria of malignancy were observed. In histological sections from the duodenopancreatectomy specimens, the parasites were observed attached to the epithelium, but individual Giardia parasites were also observed beneath the epithelial lining. According to conventional genotyping, the infecting Giardia belonged to sub-assemblage AII.
- Klíčová slova
- Adenocarcinoma, Cytology, EUS-FNA, Giardia, Pancreas, Sub-assemblage AII,
- MeSH
- asymptomatické infekce * MeSH
- duodenum cytologie parazitologie MeSH
- epitelové buňky parazitologie MeSH
- Giardia lamblia genetika izolace a purifikace MeSH
- giardiáza komplikace diagnóza MeSH
- lidé MeSH
- nádory slinivky břišní komplikace parazitologie MeSH
- náhodný nález * MeSH
- senioři MeSH
- trofozoiti izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
The objective of this work was to compare the effect of selected feed mixtures on the duodenal morphology. One-hundred-four rats of the Wistar strain were divided to thirteen groups per eight rats. The experiment started in 35-day-old rats after birth and lasted for 32 days. The groups (A-M) were fed by commercial diet, 85 % wheat and 15 % oat diet, 85 % wheat and 15 % triticale, 85 % wheat and 15 % barley, 85 % wheat and 15 % amaranth, 85 % wheat and 15 % lantern, 85 % wheat and 15 % buckwheat, 100 % wheat, 100 % white lupine, 100 % flock peas - variety Garden, 100 % native peas - variety Garden, 100 % native peas - variety Zekon or 100 % extruded peas - variety Zekon diet, respectively. Samples from the duodenum were taken. The height of the villi and the depth of the crypts were measured. The tallest villi were measured in group F (474.33+/-114.36 microm) and the shortest villi were observed in group B (294.08+/-88.52 microm). The deepest crypts were measured in group K (166.41+/-35.69 microm) and the shallowest crypts were observed in group E (77.85+/-17.61 microm). The work documents that gluten-free and classical cereals combination can be a better choice for people who want to limit the gluten content of the diet.
- MeSH
- bezlepková dieta metody MeSH
- duodenum cytologie patologie MeSH
- hrách setý * škodlivé účinky MeSH
- jedlá semena * škodlivé účinky MeSH
- krmivo pro zvířata * škodlivé účinky MeSH
- krysa rodu Rattus MeSH
- potkani Wistar MeSH
- střevní sliznice cytologie patologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
In last few years, numerous groups of proteins participating in the regulation of cell proliferation, differentiation and death during ontogenesis have been described. In this study we compared the occurrence of Bcl-2, p53 and myc protein families with the level of proliferative activity and apoptosis during development of duodenal epithelium. Paraffin embedded tissues of eight human embryos and foetuses aged from the 6th-18th week of IUD were used. For the detection of apoptotic cells the TUNEL method was performed, the proliferative marker PCNA and all the proteins studied were detected by means of indirect three-step immunohistochemical method. In the 6th and 8th week of intrauterine development we observed isolated TUNEL positive epithelial cells only and this was accompanied by the disperse presence of PCNA as well as by all the studied proteins: Bcl-2, Bax, Bcl-XL, c-myc, N-myc, p53, p63 and p73. In the early foetal period of duodenal development we registered changes in PCNA and TUNEL positivity in accordance with the constitution of the stem cell pool on base of villi, where more numerous Bcl-2 positive cells were also found. The separation of primitive crypts and villi was not accompanied by any differences in distribution of Bax, Bcl-XL, c-myc, N-myc, p63 and p73 proteins between those compartments: all the studied proteins showed dispersed character. P53 rapidly decreased in this period. In the 18th week of intrauterine development the balance between proliferation in crypts and apoptosis of villi epithelium was well established and no p53 positive cells were found. In the presence of Bcl-2, Bax, Bcl-XL, p63 and p73 we did not find any dramatic changes. The myc proteins were restricted within the epithelium of the Lieberkuhn crypts only.
- MeSH
- apoptóza MeSH
- duodenum cytologie embryologie metabolismus MeSH
- embryo savčí metabolismus MeSH
- epitel embryologie metabolismus MeSH
- imunohistochemie MeSH
- lidé MeSH
- nádorový supresorový protein p53 analýza MeSH
- proliferace buněk MeSH
- protoonkogenní proteiny c-bcl-2 analýza MeSH
- protoonkogenní proteiny c-myc analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nádorový supresorový protein p53 MeSH
- protoonkogenní proteiny c-bcl-2 MeSH
- protoonkogenní proteiny c-myc MeSH
BACKGROUND: Even though coffee is not considered to be responsible for development of peptic ulcer, it may, however, prolong its healing by increasing acidity of gastric content. In our former work we observed a profound increase in sucrose permeability (above normal values) in healthy volunteers regularly drinking coffee for years. In literature, many factors affecting sucrose permeability have been described so far. None of them, however, studied the effect of coffee. SUBJECTS, MATERIALS AND METHODS: 10 young asymptomatic habitual coffee drinkers were included in the study. The probands underwent SaLM test twice--first time without coffee restriction and second time after 48-hour coffee abstinence. The ingested SaLM solution comprised sucrose (25.0 g), lactulose (10.0 g), mannitol (2.0 g), xylose (2.0 g) and water (up to 100 ml). Urine was collected for five hours and the samples were analysed using gas chromatography. Results were compared with those of 8 young healthy volunteers not drinking coffee. Permeability for sucrose was significantly higher in the group of habitual coffee drinkers in comparison with non-coffee drinkers (p < 0.01). After 48-hour coffee abstinence sucrose excretion decreased significantly (p < 0.05) to a level not differing from that of non-coffee drinkers (p = 0.54). CONCLUSIONS: Our results indicate that coffee may damage gastroduodenal mucosa in habitual coffee drinkers. In a time period of 48 hours the gastroduodenal mucosa is capable of a significant regeneration.
- MeSH
- dospělí MeSH
- duodenum cytologie fyziologie MeSH
- káva škodlivé účinky MeSH
- lidé MeSH
- permeabilita MeSH
- sacharosa moč MeSH
- střevní sliznice fyziologie MeSH
- žaludeční sliznice fyziologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- káva MeSH
- sacharosa MeSH
In the present work the effects of fasting and refeeding on fat pad weight and alkaline phosphatase activity in the brush border of individual duodenal enterocytes have been evaluated in male Wistar rats with obesity induced by monosodium glutamate (MSG) treatment during the early postnatal period. Neonatal rats were treated subcutaneously with MSG (2 mg/g b.w.) or saline (controls) for 4 days after birth. At 4 months of age, two types of experiments were performed. In the first experiment rats, were submitted to 3 or 6 days lasting food deprivation. In the second experiment the rats were refed for 3 or 6 days ad libitum or restrictedly (60% of pre-fasting intake) after a 6 day-fasting period. Fasting and refeeding influenced the body fat and function of the duodenum in MSG-treated rats differently as compared to the controls. However, alkaline phosphatase activity and the weight of epididymal and retroperitoneal fat depots were significantly increased in MSG obese rats (P<0.001) during all the periods examined. While 3 days of food deprivation resulted in both groups in a similar loss of adipose tissue weight and alkaline phosphatase activity, the decrements of these parameters after 6 days of fasting were lower in obese rats suggesting that their capacity to spare body fat stores was enhanced. After 3 days of ad libitum refeeding, a more marked adaptational increase of food consumption and also a significantly increased alkaline phosphatase activity above the pre-fasting level (P<0.01) was observed in the MSG-treated rats. Consequently, a more rapid body fat restoration was demonstrated in these animals. Refeeding of rats at 60% of the pre-fasting intake level resulted in a significant increase of alkaline phosphatase activity in both the MSG and control group; moreover, as food restriction continued, MSG-treated rats tended to further increase the enzyme activity. Our results revealed that MSG treatment of neonatal rats may significantly change the intestinal functions. Permanently increased alkaline phosphatase activity observed in MSG obese rats during all investigated periods suggests that this functional alteration is probably not a consequence of actual nutritional variation but could be a component of regulatory mechanisms maintaining their obesity at critical values.
- MeSH
- alkalická fosfatasa metabolismus MeSH
- duodenum cytologie enzymologie MeSH
- enterocyty enzymologie MeSH
- glutamát sodný MeSH
- krysa rodu Rattus MeSH
- novorozená zvířata MeSH
- obezita chemicky indukované metabolismus MeSH
- omezení příjmu potravy fyziologie MeSH
- potkani Wistar MeSH
- potravinářské přísady MeSH
- přijímání potravy fyziologie MeSH
- tuková tkáň anatomie a histologie MeSH
- velikost orgánu MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkalická fosfatasa MeSH
- glutamát sodný MeSH
- potravinářské přísady MeSH
The day-night variation of food intake and alkaline phosphatase (AP) activity was studied in the duodenum of rats neonatally treated with monosodium glutamate (MSG) and saline-treated (control) rats. The animals were kept under light-dark conditions (light phase from 09:00 h to 21:00 h) with free access to food. AP activity was cytophotometrically analyzed in the brush-border of enterocytes separated from the tip, middle and cryptal part of the villi every 6 h over a 24-hour period. In comparison with the controls, MSG-treated rats consumed about 40% less food during the dark period and their 24-hour food intake was thus significantly lowered (P<0.001). On the other hand, the nocturnal feeding habit showed a similar pattern: food consumption was high during the night (65% vs. 75%) and the lowest consumption was found during the light phase (35% vs. 25%) in MSG-treated and control rats, respectively. In agreement with the rhythm of food intake, the highest AP activity was observed during the dark phase and was lowest during the light phase in both groups of animals. These significant day-night variations showed nearly the same pattern in the enterocytes of all observed parts along the villus axis. In comparison with the controls, a permanent increase of AP activity was observed in neonatal MSG-treated rats. This increase was more expressive during the dark phase of the day in the cryptal (P<0.001) and middle part of the villus (P<0.01). From the viewpoint of feeding, this enzyme in MSG-treated rats was enhanced in an inverse relation to the amount of food eaten i.e. despite sustained hypophagia the mean AP activity in the enterocytes along the villus axis was higher than in the control animals during all investigated periods. The present results suggest that the increased AP activity in MSG-treated rats is probably not a consequence of actual day-night eating perturbations but could be a component of a more general effect of MSG. This information contributes to better understanding of the function of intestinal AP and its relation to day-night feeding changes especially in connection with the MSG syndrome.
- MeSH
- aktivace enzymů účinky léků fyziologie MeSH
- alkalická fosfatasa metabolismus MeSH
- cirkadiánní rytmus fyziologie MeSH
- duodenum cytologie enzymologie MeSH
- enterocyty účinky léků enzymologie MeSH
- glutamát sodný farmakologie MeSH
- intestinální absorpce účinky léků fyziologie MeSH
- krysa rodu Rattus MeSH
- mikroklky enzymologie MeSH
- novorozená zvířata MeSH
- obezita metabolismus MeSH
- osvětlení MeSH
- potkani Wistar MeSH
- potravinářské přísady farmakologie MeSH
- přijímání potravy fyziologie MeSH
- tma MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkalická fosfatasa MeSH
- glutamát sodný MeSH
- potravinářské přísady MeSH
In the present work neonatal male and female Wistar rats were treated intraperitoneally with monosodium glutamate (MSG 2 mg/kg b.w.) or saline (controls) daily for 4 day after birth. At the age of 30 and 80 days, the alkaline phosphatase activity (AP) in the brush border of individual enterocytes, the body fat content and Lee's index of obesity were analyzed. Microdensitometrical quantification of AP was significantly increased on day 30 in males (P<0.01) and on day 80 in MSG-treated male and female rats (P<0.001) as compared to the controls. MSG administration also increased the body fat weight and the obesity index significantly (P<0.001) in 80-day-old animals, but was without any significant effect on their food intake. Our results showed that a) neonatal MSG-treatment may significantly change the intestinal function and b) the investigation of the intestinal enzyme activities may be important in further studies on MSG-induced and other forms of obesity.
- MeSH
- alkalická fosfatasa metabolismus MeSH
- duodenum cytologie účinky léků enzymologie MeSH
- enterocyty enzymologie MeSH
- glutamát sodný farmakologie MeSH
- injekce intraperitoneální MeSH
- krysa rodu Rattus MeSH
- novorozená zvířata růst a vývoj metabolismus MeSH
- obezita chemicky indukované patologie MeSH
- potkani Wistar MeSH
- referenční hodnoty MeSH
- stárnutí metabolismus MeSH
- tkáňová distribuce MeSH
- tuková tkáň patologie MeSH
- velikost orgánu účinky léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- alkalická fosfatasa MeSH
- glutamát sodný MeSH
A protocol for the handling of small intestinal biopsies from HIV-infected patients is presented. This protocol includes the Warthin-Starry stain for the detection of microsporidia. This stain has proved a reliable and sensitive diagnostic technique for microsporidial infections as it stains both Enterocytozoon bieneusi and Septata intestinalis in duodenal enterocytes. Because the stain demonstrates Septata intestinalis in lamina propria macrophages as well as enterocytes, it allows for the practical differentiation of these two microsporidial infections. The Warthin-Starry stain has also demonstrated Septata intestinalis in nasal and colonic biopsies in some of these patients. Since the completion of an earlier study, a further 40 cases of Enterocytozoon bieneusi and three cases of Septata intestinalis have been diagnosed in just over 240 consecutive duodenal biopsies from HIV positive patients presenting with diarrhoea and other gastrointestinal complaints. Other opportunistic infections include cytomegalovirus in four cases, mycobacteria in eight cases, cryptosporidia in nine cases, giardia in four cases and Isospora belli in one case. Since the ratio of these opportunistic infections has remained much the same as in the previous study of 180 consecutive duodenal biopsies, we suggest that these rates may reflect the actual prevalence of microsporidial infections in AIDS patients in Sydney, Australia.
- MeSH
- biopsie MeSH
- dospělí MeSH
- duodenum cytologie MeSH
- histocytochemie MeSH
- lidé MeSH
- střevní sliznice cytologie MeSH
- žaludeční sliznice cytologie metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- buněčné dělení účinky léků MeSH
- DNA biosyntéza MeSH
- duodenum cytologie MeSH
- játra cytologie MeSH
- krysa rodu Rattus MeSH
- nukleové kyseliny metabolismus MeSH
- techniky in vitro MeSH
- thymidin farmakologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA MeSH
- nukleové kyseliny MeSH
- thymidin MeSH
