AIMS: To evaluate whether patients with late complications of pacemakers or implantable cardioverter-defibrillators have hypersensitivity reactions to some of the materials used in generators or in electrodes, or to environmental metal burden. METHODS AND RESULTS: The cohort consisted of 20 men and 4 women (mean age: 62.3 ± 17.2 years) who had a history of late complications of implanted devices. The control group involved 25 men and 8 women (mean age: 64.6 ± 14.0 years) who had comparable devices, but no history of late complications. Lymphocyte transformation test was used to evaluate hypersensitivity to eight metal pollutants (antimony, manganese, mercury, molybdenum, nickel, platinum, tin, and titanium) selected by results of questionnaires on environmental burden, and by material analysis of generators and electrode surfaces. Exposures to metal pollutants were approximately the same in patients and in controls. Titanium alloy used in generators contained at least 99.32% of titanium and trace levels of other metals; higher levels of tin and platinum were detected in electrode surfaces. Hypersensitivity reactions to mercury and tin were significantly more frequent in patients than in controls (patients and controls: mercury: 68.2 and 31.1%, respectively; P = 0.022; tin: 25.0 and 3.2%, respectively; P = 0.035). In contrast, hypersensitivity to manganese was significantly more frequent in controls than in patients (patients and controls: 13.6 and 50.0%, respectively; P = 0.008). CONCLUSION: Our findings suggest a possible relation between hypersensitivity to metals used in implantable devices or to environmental metal burden and the occurrence of their late complications.
- MeSH
- aktivace lymfocytů MeSH
- alergie epidemiologie etiologie MeSH
- antimon škodlivé účinky MeSH
- cín škodlivé účinky MeSH
- defibrilátory implantabilní škodlivé účinky MeSH
- implantace protézy * MeSH
- infekce spojené s protézou epidemiologie MeSH
- kovy škodlivé účinky MeSH
- kožní nemoci epidemiologie MeSH
- látky znečišťující životní prostředí škodlivé účinky MeSH
- lidé středního věku MeSH
- lidé MeSH
- mangan škodlivé účinky MeSH
- molybden škodlivé účinky MeSH
- nikl škodlivé účinky MeSH
- platina škodlivé účinky MeSH
- pooperační bolest epidemiologie MeSH
- pooperační komplikace epidemiologie MeSH
- prostředky srdeční resynchronizační terapie škodlivé účinky MeSH
- rtuť škodlivé účinky MeSH
- senioři MeSH
- slitiny MeSH
- titan škodlivé účinky MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antimon MeSH
- cín MeSH
- kovy MeSH
- látky znečišťující životní prostředí MeSH
- mangan MeSH
- molybden MeSH
- nikl MeSH
- platina MeSH
- rtuť MeSH
- slitiny MeSH
- titan MeSH
Impact of manganese (Mn(2+)) excess (100, 500 and 1000 μM over 7 days) on two ontogenetic stages (7-week-old plants and 7-day-old seedlings) of Matricaria chamomilla was compared. Mn excess depressed growth of seedlings (but not germination) and stimulated oxidative stress (ROS and lipid peroxidation) in both plants and seedlings. Growth inhibition could be evoked by higher Mn uptake and higher translocation factor in seedlings than in plants. Total thiols staining revealed elevation in almost all treatments. In 7-week-old plants, activity of peroxidases increased slightly and rather decreased under high Mn doses. Superoxide rather than hydrogen peroxide contributed to visualized ROS presence. Fluorescence of nitric oxide (NO) showed stimulation in plants but decrease in seedlings. Impact of exogenous nitric oxide donor (sodium nitroprusside/SNP) was therefore tested and results showed amelioration of 1000 μM Mn-induced oxidative stress in seedlings (decrease in H2O2 and increase in NO content while antioxidative enzyme activities were variably affected) concomitantly with depleted Mn accumulation. It is concluded that NO participates in tolerance to Mn excess but negative effects of the highest SNP dose were also observed. Extensive fluorescence microscopy is also explanatively discussed.
- Klíčová slova
- Fluorescence microscopy, Heavy metals, Mineral nutrients, Ontogenesis, Reactive oxygen species,
- MeSH
- antioxidancia metabolismus MeSH
- donory oxidu dusnatého farmakologie MeSH
- fluorescenční mikroskopie metody MeSH
- fyziologická adaptace * MeSH
- mangan škodlivé účinky metabolismus MeSH
- Matricaria účinky léků růst a vývoj metabolismus MeSH
- nitroprusid farmakologie MeSH
- oxid dusnatý metabolismus farmakologie MeSH
- oxidační stres * MeSH
- peroxid vodíku metabolismus MeSH
- peroxidasy metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- semenáček účinky léků růst a vývoj metabolismus MeSH
- sulfhydrylové sloučeniny metabolismus MeSH
- superoxidy metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- donory oxidu dusnatého MeSH
- mangan MeSH
- nitroprusid MeSH
- oxid dusnatý MeSH
- peroxid vodíku MeSH
- peroxidasy MeSH
- reaktivní formy kyslíku MeSH
- sulfhydrylové sloučeniny MeSH
- superoxidy MeSH
Slovakia is characterised by an unusually high number of patients affected by genetic Creutzfeldt-Jakob disease (CJD) with E200K mutation at the PRNP gene. Penetrance of the mutation is incomplete (59%). Therefore, for the onset of the clinical manifestation, an influence of other endo- or exogenous factors could not be excluded. Experimental data suggest that copper and manganese levels may play an important role in the pathogenesis of prion diseases. The highest number of Slovak genetic CJD patients originates from Orava - the northern region of central Slovakia. Manganese is a dominant pollutant in Orava. The objective of this study was to clarify a possible exogenous influence of environmental Mn/Cu imbalance on the CJD clustering. Mn and Cu levels were analysed in the brain tissue of genetic CJD cases (from Orava and from control regions of Slovakia), as well as of sporadic CJD patients and controls. Analyses demonstrate i) significantly higher Mn level in focally accumulated, "clustering" genetic CJD cases in comparison to all other groups, ii) Cu status differences between compared groups were without statistical significance; decreased concentrations were found in genetic cases from extrafocal genetic CJD areas, iii) Mn/Cu ratios were increased in all CJD groups in comparison to controls. Metal ratios in clustering gCJD cases were significantly higher in comparison to sporadic cases and also to controls, but not to the extrafocal genetic CJD subgroup. These results indicate that more important than increasing Mn level in pathogenesis of CJD appears to be the role of the Mn/Cu imbalance in the CNS. The imbalance observed in the cluster of genetic CJD cases is probably a result of both: the excessive environmental Mn level and the disturbance of Mn/Cu ratios in the Orava region. Presented findings indicate an environmental Mn/Cu imbalance as a possible exogenous CJD risk co-factor which may, in coincidence with endogenous (genetic) CJD risk, contribute to the focal accumulation (cluster) of genetic CJD in Slovakia.
- MeSH
- Creutzfeldtova-Jakobova nemoc epidemiologie etiologie genetika MeSH
- lidé MeSH
- mangan škodlivé účinky analýza MeSH
- měď škodlivé účinky analýza MeSH
- mozek - chemie MeSH
- polymerázová řetězová reakce MeSH
- polymorfismus genetický MeSH
- prionová bílkovina MeSH
- priony genetika MeSH
- rizikové faktory MeSH
- shluková analýza MeSH
- studie případů a kontrol MeSH
- vystavení vlivu životního prostředí škodlivé účinky MeSH
- zeměpis MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika epidemiologie MeSH
- Názvy látek
- mangan MeSH
- měď MeSH
- prionová bílkovina MeSH
- priony MeSH
- PRNP protein, human MeSH Prohlížeč
The concentration of manganese was determined in the work environment of eight enterprises by welding and metal processing (198 samples) as well as in biological samples (blood, hair) of workers (180 samples). Industrially exposed individuals had significantly higher levels of manganese in blood and hair than the control group--7.6 times higher in blood and 3.2 times higher in hair. Neurobehavioral examination was performed by physician and specific questionnaire (Q16) containing 16 "yes" or "no" items relating to neuropsychiatric symptoms. It is possible to use the level of manganese in biosamples as the indicator of health risk by occupationally exposed individuals.
- MeSH
- hodnocení rizik * MeSH
- lidé MeSH
- mangan škodlivé účinky analýza krev MeSH
- pracovní expozice MeSH
- svařování MeSH
- vlasy, chlupy chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Lotyšsko MeSH
- Názvy látek
- mangan MeSH
Occupational environment monitoring and biological-medical monitoring of persons professionally exposed to welding fumes have been performed. Chromium, manganese and polycyclic aromatic hydrocarbons in welding fumes represents an important health risk. Pollutant concentrations found in metal welding fumes represented only fractions of those acceptable ones. Polycyclic aromatic hydrocarbons have been reached the concentration found in a busy road crossing in Hradec Králové (compared with these as in Czech Republic no maximum acceptable levels for PAHs having been declared). Family, personal and occupational history have been taken. Health state including total haematological count, biochemical and cytogenetical changes of 19 stainless steel welders were checked-up. The level of mercapturates in urine were examined as well. The data were statistically compared with those of non exposed (control group). No changes witnessing the above mentioned risk factors influence on the haematological, biochemical and cytogenetical findings were ever proved. In conclusion, our results did not confirm an increased professional risk in this group of welders.
- MeSH
- chrom škodlivé účinky MeSH
- chromozomální aberace MeSH
- dospělí MeSH
- krevní glukóza analýza MeSH
- krevní obraz účinky léků MeSH
- látky znečišťující vzduch v pracovním prostředí škodlivé účinky MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipidy krev MeSH
- mangan škodlivé účinky MeSH
- nikl škodlivé účinky MeSH
- polycyklické aromatické uhlovodíky škodlivé účinky MeSH
- pracovní expozice * MeSH
- svařování * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- chrom MeSH
- krevní glukóza MeSH
- látky znečišťující vzduch v pracovním prostředí MeSH
- lipidy MeSH
- mangan MeSH
- nikl MeSH
- polycyklické aromatické uhlovodíky MeSH
74 healthy welders were subjected to immunological screening. Serum immunoglobulins (IgG, IgA, IgM) were measured as well as total and active E-RFC and cellular immunity was evaluated using the intradermal testing of PPD, candidin D-7, trichophytin D-5 and tetanus anatoxin. The obtained results suggest suppression of the T and B lymphocyte immune systems as expressed by decreased levels of serum IgG and total E-RFC. Cell-mediated immune deficiency was found in 21.6% welders. Suppression of the immune system may be accounted for by a complex effect of occupational factors, such as manganese compounds, vibration and noise.
- MeSH
- B-lymfocyty imunologie MeSH
- dospělí MeSH
- imunoglobulin G analýza MeSH
- imunoglobuliny analýza MeSH
- imunologická tolerance MeSH
- lidé středního věku MeSH
- lidé MeSH
- mangan škodlivé účinky MeSH
- monitorování imunologické MeSH
- svařování * MeSH
- T-lymfocyty imunologie MeSH
- vystavení vlivu životního prostředí MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Bulharsko MeSH
- Názvy látek
- imunoglobulin G MeSH
- imunoglobuliny MeSH
- mangan MeSH
- MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mangan škodlivé účinky MeSH
- nemoci nervového systému chemicky indukované diagnóza MeSH
- nemoci z povolání chemicky indukované diagnóza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- mangan MeSH
- MeSH
- dospělí MeSH
- elektroencefalografie * MeSH
- hutnictví * MeSH
- lidé středního věku MeSH
- lidé MeSH
- mangan škodlivé účinky MeSH
- světelná stimulace MeSH
- vystavení vlivu životního prostředí MeSH
- zaměstnání MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- mangan MeSH
- Klíčová slova
- EYE/wounds and injuries *, MANGANESE/injurious effects *,
- MeSH
- hlava * MeSH
- mangan škodlivé účinky MeSH
- oči * MeSH
- popálení * MeSH
- poranění oka * MeSH
- sloučeniny manganu * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- mangan MeSH
- sloučeniny manganu * MeSH
