The antibacterial effects of essential oils and their components (EOCs) are usually attributed to effects on membranes and metabolism. Studies of the effects of EOCs on protein expression have primarily analysed proteins larger than 10 kDa using gel electrophoresis. In the present study, we used MALDI-TOF-MS to investigate the effects of EOCs on low-molecular-weight proteins. From 297 m/z features, we identified 94 proteins with important differences in expression among untreated samples, samples treated with EOCs, and samples treated with antibiotics, peroxide, or chlorine. The targets of these treatments obviously differ, even among EOCs. In addition to ribosomal proteins, stress-, membrane- and biofilm-related proteins were affected. These findings may provide a basis for identifying new targets of essential oils and synergies with other antibiotics.
- MeSH
- antibakteriální látky metabolismus MeSH
- Escherichia coli účinky léků fyziologie MeSH
- fyziologický stres * MeSH
- molekulová hmotnost MeSH
- oleje prchavé metabolismus MeSH
- proteiny z Escherichia coli analýza chemie MeSH
- proteom analýza MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- oleje prchavé MeSH
- proteiny z Escherichia coli MeSH
- proteom MeSH
Recently, a growing number of plant essential oils (EOs) have been tested against a wide range of arthropod pests with promising results. EOs showed high effectiveness, multiple mechanisms of action, low toxicity on non-target vertebrates and potential for the use of byproducts as reducing and stabilizing agents for the synthesis of nanopesticides. However, the number of commercial biopesticides based on EOs remains low. We analyze the main strengths and weaknesses arising from the use of EO-based biopesticides. Key challenges for future research include: (i) development of efficient stabilization processes (e.g., microencapsulation); (ii) simplification of the complex and costly biopesticide authorization requirements; and (iii) optimization of plant growing conditions and extraction processes leading to EOs of homogeneous chemical composition.
- Klíčová slova
- botanical pesticides, microencapsulation, nanosynthesis, natural product research, stabilization processes,
- MeSH
- biologická ochrana metabolismus MeSH
- biologické přípravky metabolismus MeSH
- oleje prchavé metabolismus MeSH
- oleje rostlin metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- biologická ochrana MeSH
- biologické přípravky MeSH
- oleje prchavé MeSH
- oleje rostlin MeSH
Research on the role of arbuscular mycorrhizal fungi (AMF) in the synthesis of essential oils (EOs) by aromatic plants has seldom been conducted in field-relevant conditions, and then, only limited spectra of EO constituents have been analyzed. The effect was investigated of inoculation with AMF on the synthesis of a wide range of EO in two aromatic species, coriander (Coriandrum sativum) and dill (Anethum graveolens), in a garden experiment under outdoor conditions. Plants were grown in 4-l pots filled with soil, which was either γ-irradiated (eliminating native AMF) or left non-sterile (containing native AMF), and inoculated or not with an isolate of Rhizophagus irregularis. AMF inoculation significantly stimulated EO synthesis in both plant species. EO synthesis (total EO and several individual constituents) was increased in dill in all mycorrhizal treatments (containing native and/or inoculated AMF) compared to non-mycorrhizal plants. In contrast, EO concentrations in coriander (total EO and most constituents) were increased only in the treatment combining both inoculated and native AMF. A clear positive effect of AMF on EO synthesis was found for both aromatic plants, which was, however, specific for each plant species and modified by the pool of AMF present in the soil.
- Klíčová slova
- Arbuscular mycorrhiza, Aromatic plants, Coriander, Dill, Essential oils, Growth responses,
- MeSH
- kopr vonný metabolismus mikrobiologie MeSH
- koriandr metabolismus mikrobiologie MeSH
- mykorhiza růst a vývoj MeSH
- oleje prchavé metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- oleje prchavé MeSH
The paper deals with the evaluation of the content and quality of the essential oil of Melissa officinalis in various leaf insertions in the course of the ontogenetic development of the plant. The quality of the drug after the administration of the preparation Rastin 30 DKV in the form of granules was not changed in comparison with control samples. The experimental work, subsequent analysis of samples, and evaluation of results led to conclusions important from the viewpoint of significant changes in the content and composition of the essential oil taking place in the plant in the course of the vegetation period.
- MeSH
- benzothiazoly MeSH
- léčivé rostliny metabolismus MeSH
- oleje prchavé chemie metabolismus MeSH
- regulátory růstu rostlin farmakologie MeSH
- thiazoly farmakologie MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- benzothiazoly MeSH
- oleje prchavé MeSH
- Rastim 30 DKV MeSH Prohlížeč
- regulátory růstu rostlin MeSH
- thiazoly MeSH
