Tryptophan-derived metabolites, a group of neurotransmitters essential for various brain functions, play key roles in regulating mood, movement, sleep, and cognition. However, the comprehensive characterisation of tryptophan-melatonin pathway metabolites is challenging due to factors such as their structural diversity, chemical complexity, low concentrations, and instability of these metabolites. In this study, we developed and validated an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) methodology with electrospray ionisation for the simultaneous separation and quantification of tryptophan metabolites in human plasma. The analytical calibration ranges in plasma were 0.50-200 ng/mL for serotonin, 0.01-5 ng/mL for N-acetylserotonin, 0.01-20 ng/mL for tryptamine, 0.01-20 ng/mL for 6-sulfatoxymelatonin, 0.01-20 ng/mL for 6-hydroxymelatonin, 0.01-100 ng/mL for melatonin, and 0.10-20 ng/mL for N-acetyltryptamine, with correlation coefficients ranging from 0.954 for N-acetyltryptamine to 0.997 for tryptamine. The intraday and interday precision remained consistently below 15 % for all analytes. Most analytes met the accuracy criteria, except for N-acetyltryptamine at the lowest quality control level (0.2 ng/mL), where the intraday and interday accuracy were 22.4 % and 17.4 %, respectively. In conclusion, this novel method allows for rapid identification of tryptophan-melatonin pathway intermediates in less than ten minutes, including seven distinct melatonin-related analytes. This suggests that it may find use in everyday clinical and scientific endeavours.
- Klíčová slova
- Liquid chromatography, Mass spectrometry, Melatonin, Plasma, Tryptophan metabolites,
- MeSH
- lidé MeSH
- limita detekce MeSH
- lineární modely MeSH
- melatonin * krev analogy a deriváty metabolismus chemie MeSH
- reprodukovatelnost výsledků MeSH
- serotonin krev analogy a deriváty MeSH
- tandemová hmotnostní spektrometrie * metody MeSH
- tryptaminy krev MeSH
- tryptofan * krev metabolismus chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- melatonin * MeSH
- N-acetylserotonin MeSH Prohlížeč
- serotonin MeSH
- tryptaminy MeSH
- tryptofan * MeSH
The trichothecene mycotoxins contaminate cereal grains and have been related to alimentary toxicosis resulted in emetic response. This family of mycotoxins comprises type A to D groups of toxic sesquiterpene chemicals. Diacetoxyscirpenol (DAS), one of the most toxic type A trichothecenes, is considered to be a potential risk for human and animal health by the European Food Safety Authority. Other type A trichothecenes, T-2 toxin and HT-2 toxin, as well as type B trichothecene deoxynivalenol (DON), have been previously demonstrated to induce emetic response in the mink, and this response has been associated with the plasma elevation of neurotransmitters peptide YY (PYY) and serotonin (5-hydroxytryptamine, 5-HT). However, it is found that not all the type A and type B trichothecenes have the capacity to induce PYY and 5-HT. It is necessary to identify the roles of these two emetogenic mediators on DAS-induced emesis. The goal of this study was to determine the emetic effect of DAS and relate this effect to PYY and 5-HT, using a mink bioassay. Briefly, minks were fasted one day before experiment and given DAS by intraperitoneally and orally dosing on the experiment day. Then, emetic episodes were calculated and blood collection was employed for PYY and 5-HT test. DAS elicited robust emetic responses that corresponded to upraised PYY and 5-HT. Blocking the neuropeptide Y2 receptor (NPY2R) diminished emesis induction by PYY and DAS. The serotonin 3 receptor (5-HT3R) inhibitor granisetron totally restrained the induction of emesis by serotonin and DAS. In conclusion, our findings demonstrate that PYY and 5-HT have critical roles in DAS-induced emetic response.
- Klíčová slova
- diacetoxyscirpenol, emesis, mycotoxin, peptide YY, serotonin, trichothecene,
- MeSH
- antagonisté serotoninových receptorů 5-HT3 farmakologie MeSH
- antiemetika farmakologie MeSH
- granisetron farmakologie MeSH
- modely nemocí na zvířatech MeSH
- norek MeSH
- peptid YY krev MeSH
- receptory gastrointestinálních hormonů metabolismus MeSH
- receptory serotoninové 5-HT3 metabolismus MeSH
- sekreční dráha MeSH
- serotonin krev MeSH
- trichotheceny * MeSH
- upregulace MeSH
- zvířata MeSH
- zvracení krev chemicky indukované prevence a kontrola MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antagonisté serotoninových receptorů 5-HT3 MeSH
- antiemetika MeSH
- diacetoxyscirpenol MeSH Prohlížeč
- granisetron MeSH
- peptid YY MeSH
- peptide YY receptor MeSH Prohlížeč
- receptory gastrointestinálních hormonů MeSH
- receptory serotoninové 5-HT3 MeSH
- serotonin MeSH
- trichotheceny * MeSH
BACKGROUND: During blood feeding, sand flies inoculate salivary proteins that interact with the host haemostatic system. The blocking of biogenic amines such as serotonin and histamine helps to limit vasodilatation and clot formation, and thus enables the insect to finish the blood-feeding process. In sand flies, an amine-binding ability is known only for the yellow-related proteins of Phlebotomus and Lutzomyia vectors, but not yet for members of the genus Sergentomyia. METHODS: The ability of Phlebotomus argentipes and Sergentomyia schwetzi recombinant yellow-related salivary proteins to bind histamine and serotonin was measured by microscale thermophoresis. Both sand fly species were also fed through a chicken-skin membrane on blood mixed with histamine or serotonin in order to check the effects of biogenic amines on sand fly fitness. Additionally, fecundity and mortality were compared in two groups of P. argentipes females fed on repeatedly-bitten and naive hamsters, respectively. RESULTS: The P. argentipes recombinant yellow-related protein PagSP04 showed high binding affinity to serotonin and low affinity to histamine. No binding activity was detected for two yellow-related proteins of S. schwetzi. Elevated concentrations of serotonin significantly reduced the amount of eggs laid by P. argentipes when compared to the control. The fecundity of S. schwetzi and the mortality of both sand fly species were not impaired after the experimental membrane feeding. Additionally, there were no differences in oviposition or mortality between P. argentipes females fed on immunized or naive hamsters. CONCLUSIONS: Our results suggest that in natural conditions sand flies are able to cope with biogenic amines or anti-saliva antibodies without any influence on their fitness. The serotonin binding by salivary yellow-related proteins may play an important role in Phlebotomus species feeding on mammalian hosts, but not in S. schwetzi, which is adapted to reptiles.
- Klíčová slova
- Anti-saliva antibodies, Histamine, Mortality, Oviposition, Phlebotomus argentipes, Sergentomyia schwetzi, Serotonin, Yellow-related proteins,
- MeSH
- biogenní aminy * krev farmakologie MeSH
- fertilita účinky léků MeSH
- histamin krev MeSH
- hmyzí proteiny chemie metabolismus MeSH
- kousnutí a bodnutí hmyzem imunologie MeSH
- křečci praví MeSH
- krev metabolismus MeSH
- molekulární evoluce MeSH
- mortalita MeSH
- Phlebotomus metabolismus MeSH
- plazi MeSH
- protilátky MeSH
- Psychodidae metabolismus MeSH
- rekombinantní proteiny chemie metabolismus MeSH
- savci MeSH
- serotonin krev MeSH
- slinné proteiny a peptidy * chemie metabolismus MeSH
- sliny imunologie MeSH
- vazba proteinů MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biogenní aminy * MeSH
- histamin MeSH
- hmyzí proteiny MeSH
- protilátky MeSH
- rekombinantní proteiny MeSH
- serotonin MeSH
- slinné proteiny a peptidy * MeSH
The aim of this study was to examine the effect of alterations in potential lead fall distance on the hormonal responses of rock climbers. Nine advanced female climbers completed two routes while clipping all (PRO-all) or half (PRO-½) of the fixed points of protection. Venous blood samples were analysed for total catecholamines, noradrenaline (norepinephrine), adrenaline (epinephrine), dopamine, lactate, cortisol and serotonin. Differences between the two conditions pre, immediately post and 15 min post climbing were assessed using a 2 × 3 repeated measures ANOVA. All hormones and blood lactate concentrations increased significantly (P < 0.05) immediately post climb, except for cortisol. Peak cortisol concentrations did not occur until 15 min post ascent. Further, significant interactions between climbing and clipping conditions were found for total catecholamines (890% of basal concentration in PRO-½ vs. 568% in PRO-all), noradrenaline (794% vs. 532%) and dopamine (500% vs. 210%). There were no significant interactions for adrenaline (1920% vs. 1045%), serotonin (150% vs. 127%) or lactate (329% vs. 279%). The study showed a greater catecholamine response with an increase in potential lead fall distance. The most pronounced increases seen in catecholamine concentration were reported for dopamine and noradrenaline.
- Klíčová slova
- Catecholamines, anxiety, cortisol, rock climbing, serotonin, stress,
- MeSH
- adrenalin krev MeSH
- dopamin krev MeSH
- dospělí MeSH
- hormony krev MeSH
- horolezectví zranění fyziologie psychologie MeSH
- hydrokortison krev MeSH
- kyselina mléčná krev MeSH
- lidé MeSH
- noradrenalin krev MeSH
- odpočinek MeSH
- percepce MeSH
- serotonin krev MeSH
- srdeční frekvence fyziologie MeSH
- strach MeSH
- tělesná námaha fyziologie MeSH
- úzkost * MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- adrenalin MeSH
- dopamin MeSH
- hormony MeSH
- hydrokortison MeSH
- kyselina mléčná MeSH
- noradrenalin MeSH
- serotonin MeSH
BACKGROUND: Colorectal cancer (CRC), a heterogeneous disease that is common in both men and women, continues to be one of the predominant cancers worldwide. Lifestyle, diet, environmental factors and gene defects all contribute towards CRC development risk. Therefore, the identification of novel biomarkers to aid in the management of CRC is crucial. The aim of the present study was to identify candidate biomarkers for CRC, and to develop a better understanding of their role in tumourogenesis. METHODS: In this study, both plasma and tissue samples from patients diagnosed with CRC, together with non-malignant and normal controls were examined using mass spectrometry based proteomics and metabolomics approaches. RESULTS: It was established that the level of several biomolecules, including serotonin, gamma enolase, pyruvate kinase and members of the 14-3-3 family of proteins, showed statistically significant changes when comparing malignant versus non-malignant patient samples, with a distinct pattern emerging mirroring cancer cell energy production. CONCLUSION: The diagnosis and management of CRC could be enhanced by the discovery and validation of new candidate biomarkers, as found in this study, aimed at facilitating early detection and/or patient stratification together with providing information on the complex behaviour of cancer cells.
- Klíčová slova
- 14-3-3 Proteins, Biomarkers, Colorectal cancer, Mass spectrometry, Proteomics, Pyruvate kinase,
- MeSH
- fosfopyruváthydratasa krev metabolismus MeSH
- hmotnostní spektrometrie MeSH
- kolorektální nádory krev diagnóza MeSH
- lidé středního věku MeSH
- lidé MeSH
- metabolomika MeSH
- proteiny 14-3-3 krev metabolismus MeSH
- proteomika MeSH
- pyruvátkinasa krev metabolismus MeSH
- senioři MeSH
- serotonin krev metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfopyruváthydratasa MeSH
- proteiny 14-3-3 MeSH
- pyruvátkinasa MeSH
- serotonin MeSH
Oxidized cellulose is an effective hemostat that works naturally to aid in blood coagulation. The mechanism of its action is not very well understood. Little effect on blood coagulation, but a pronounce decrease in platelet count has been reported upon the addition of the oxidized cellulose to the whole blood. As a marker of platelet activation and aggregation we used serotonin release reaction and turbidity changes in time. We found that oxidized cellulose did not activate washed platelets reconstituted in plasma-free medium or plasma-free medium with fibrinogen; no reduction of platelet count was observed. Serotonin release in platelet-rich plasma incubated with oxidized cellulose started in the range from 5 to 10 min. Serotonin release from platelets reconstituted in plasma deficient in either coagulation factor V, VIII, IX, or XII was delayed. Blood platelets activation by oxidized cellulose requires calcium ions present in dispersion of oxidized cellulose. Factor XIII deficiency had no influence on blood platelets activation by oxidized cellulose. Our results clearly indicate the significance of intrinsic coagulation pathway activation on blood platelets activation by oxidized cellulose and so indirectly on the hemostyptic effect of oxidized cellulose.
- MeSH
- agregace trombocytů účinky léků MeSH
- aktivace trombocytů účinky léků fyziologie MeSH
- biokompatibilní materiály farmakologie MeSH
- celulosa oxidovaná farmakologie MeSH
- hemokoagulace účinky léků fyziologie MeSH
- hemostatika farmakologie MeSH
- lidé MeSH
- poruchy koagulačních proteinů krev MeSH
- serotonin krev metabolismus MeSH
- techniky in vitro MeSH
- testování materiálů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- celulosa oxidovaná MeSH
- hemostatika MeSH
- serotonin MeSH
Serotonin is involved in many of the same processes affected by cannabinoids; therefore, we investigated in vitro and in vivo effects of these drugs on the function of serotonin transporter. The effect of Delta(9)-tetrahydrocannabinol (Delta(9)-THC), endocannabinoid anandamide and synthetic cannabinoid receptor agonist WIN 55,212-2 on platelet serotonin uptake and membrane microviscosity was examined in 19 marijuana smokers and 20 controls. (1) Serotonin uptake was inhibited at higher doses of Delta(9)-THC (IC(50) = 139 micromol/l), anandamide (IC(50) = 201 micromol/l) or WIN 55,212-2 (IC(50) = 17.4 micromol/l); the inhibition was found non-competitive. Delta(9)-THC, anandamide and WIN 55,212-2 produced different effects on the membrane microviscosity. (2) Maximal velocity of platelet serotonin uptake was significantly increased in a group of chronic marijuana smokers suffering impairment of cognitive functions when compared with controls. Opposite effect of marijuana smoking on the serotonin uptake efficiency was observed in males beside females. In summary, this study provides evidence that (1) Activity of serotonin transporter is acutely affected by cannabinoids at relatively high drug concentrations; this effect is indirect and can be partially accounted for the changes in the membrane microviscosity. (2) Increase of maximal velocity of the serotonin uptake could be understood as adaptation change in the serotonergic system induced by chronic cannabis use. A hypothesis was supported that lowered serotonin uptake may reflect a gender-related differences in effects of psychoactive cannabinoids.
- MeSH
- benzoxaziny farmakologie MeSH
- buněčná membrána účinky léků MeSH
- dospělí MeSH
- endokanabinoidy MeSH
- imipramin farmakologie MeSH
- kanabinoidy farmakologie MeSH
- kokain farmakologie MeSH
- kouření marihuany krev MeSH
- kyseliny arachidonové farmakologie MeSH
- lidé MeSH
- membránové transportní proteiny pro serotonin účinky léků MeSH
- morfoliny farmakologie MeSH
- naftaleny farmakologie MeSH
- pohlavní dimorfismus MeSH
- polynenasycené alkamidy farmakologie MeSH
- serotonin krev MeSH
- techniky in vitro MeSH
- tetrahydrokanabinol farmakologie MeSH
- trombocyty účinky léků MeSH
- viskozita MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- (3R)-((2,3-dihydro-5-methyl-3-((4-morpholinyl)methyl)pyrrolo-(1,2,3-de)-1,4-benzoxazin-6-yl)(1-naphthalenyl))methanone MeSH Prohlížeč
- anandamide MeSH Prohlížeč
- benzoxaziny MeSH
- endokanabinoidy MeSH
- imipramin MeSH
- kanabinoidy MeSH
- kokain MeSH
- kyseliny arachidonové MeSH
- membránové transportní proteiny pro serotonin MeSH
- morfoliny MeSH
- naftaleny MeSH
- polynenasycené alkamidy MeSH
- serotonin MeSH
- tetrahydrokanabinol MeSH
The main objective of the study was to evaluate the endocrinological picture of anorexia. Serum leptin levels are low in untreated anorexia nervosa (AN), but studies of the exact relationship between leptin, body weight and hormones of hypothalamo-hypophyseal-thyroid axis and the impact of refeeding in anorectics are limited. The sample consistent of 15 patients with anorexia nervosa before and 1 month after partial weight recovery, and 15 age-matched control subjects. The body mass index (BMI), leptin, plasma neuropeptide Y (NPY), serotonin, thyroxine (T4), triiodothyronine (T3) and reverse triiodothyronine (rT3) in serum were evaluated for each subject. The mean serum levels of leptin, T4, and T3 were significantly lower before weight recovery in 15 patients with AN than they were in control subjects. After partial weight recovery, basal T3 levels were unchanged and significantly lower than in controls. Basal T4 was even still more reduced, but we observed significantly elevated ratio of T3/T4 and reduced ratio rT3/T4 of in AN patients after gain recovery, indicating increased conversion of T4 to T3 than to rT3. The levels of serum leptin were low in AN, but after partial weight recovery slightly increased, and correlated with BMI. No differences were observed in serum NPY. Serum levels of IGF-1 and serotonin were lower in AN than in controls before and after partial weight gain. IGF-1 was slightly increased after partial weight gain. We did not find correlation between serum levels of leptin and serum T4. The low serum levels of T3 associated with chronic starvation were thought to be the result of impaired peripheral conversion of T4 to T3. However, decreased levels of T3 were still apparent even after a partial weight gain, and the concentration of T4 was even lower. The diminished serum level of TSH in AN, however, appeared to return to the level of controls. On the basis of these results, we assume that low serum levels of thyroid hormones in AN reflect a dysfunction of the HPT axis in AN patients. It is known that in man serum serotonin levels correlate positively with T3 levels. It is possible that the low serum levels of thyroid hormones in AN subjects result in low serum serotonin and its product, melatonin. While IGF-1 reflects the energy intake of the previous few weeks, the serum leptin concentration reflects the true status of the adipose stores, a fact that has useful clinical implications.
- MeSH
- hypofýza fyziologie MeSH
- hypothalamus patofyziologie MeSH
- index tělesné hmotnosti MeSH
- insulinu podobný růstový faktor I analýza MeSH
- leptin analýza MeSH
- lidé MeSH
- mentální anorexie patofyziologie MeSH
- neuropeptid Y krev MeSH
- serotonin krev MeSH
- štítná žláza patofyziologie MeSH
- thyroxin krev MeSH
- trijodthyronin reverzní krev MeSH
- trijodthyronin krev MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- insulinu podobný růstový faktor I MeSH
- leptin MeSH
- neuropeptid Y MeSH
- serotonin MeSH
- thyroxin MeSH
- trijodthyronin reverzní MeSH
- trijodthyronin MeSH
Dichlormagnesium-aspartate-hydrochloride was given to 12 patients with mild hypertension in antihypertensive indication at a dose of 10.5 mmol Mg2+/day for 3 months. Blood pressure normalized (from 161.7 +/- 3.4/95.8 +/- 0.8 mmHg to 140.4 +/- 4.0/81.7 +/- 0.9 mmHg, after the third month (p < 0.01). While no changes in Mg2+ concentration in serum were observed in patients with normomagnesaemia, in hypomagnesaemic patients vS-Mg level normalized. Renal excretion of Mg2+ increased: from 3.41 +/- 0.36 before to 5.7 +/- 0.57 mmol Mg2+/24 h after treatment, p < 0.01. Mean plasma serotonin (5HT) concentration showed no changes, although a trend towards an increase in platelet 5HT content was observed. Elevated pre-treatment plasma 5-hydroxyindole acetic acid (5HIAA) concentrations normalized (from 137.29 +/- 20.3 to 78.96 +/- 31.64 nmol/l after the third month, p < 0.05). These findings point to a platelet-stabilizing effect of Mg2+. Fractional excretion of 5HIAA increased (from 1.42 +/- 0.27 to 5.4 +/- 1.22 after treatment, p < 0.01) while mean urinary 5HIAA excretion remained unchanged. It is deduced that a) total body 5HT and 5HIAA production was not affected; b) a long-term supplementation of Mg2+ stimulates the transport of 5HIAA in proximal tubules and, probably, intrarenal 5HIAA synthesis. A functional block in 5HT metabolism under Mg2+ treatment is anticipated. Thus, Mg2+ supplementation has renal and extrarenal effects that are important in treating hypertension and its complications.
- MeSH
- antihypertenziva terapeutické užití MeSH
- fixní kombinace léků MeSH
- hořčík krev MeSH
- hypertenze krev farmakoterapie MeSH
- krevní tlak účinky léků MeSH
- kyselina asparagová terapeutické užití MeSH
- kyselina hydroxyindoloctová moč MeSH
- lidé středního věku MeSH
- lidé MeSH
- serotonin krev MeSH
- sloučeniny hořčíku terapeutické užití MeSH
- trombocyty metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antihypertenziva MeSH
- fixní kombinace léků MeSH
- hořčík MeSH
- kyselina asparagová MeSH
- kyselina hydroxyindoloctová MeSH
- serotonin MeSH
- sloučeniny hořčíku MeSH
The effect of the calcium antagonist isradipine on serotonin metabolism and platelet aggregation was studied in 17 patients with essential hypertension. Platelet serotonin content, plasma serotonin, 5-hydroxyindoleacetic acid levels, and platelet aggregation [induced ex vivo by serotonin and low-density lipoprotein (LDL)] were measured after a 4-week placebo period and after 12 weeks of oral treatment with isradipine. Isradipine treatment significantly inhibited platelet aggregation induced by LDL and serotonin; the amplifying effect of LDL on serotonin-induced aggregation seen with placebo was not observed after 12 weeks of treatment with isradipine. Platelet serotonin content increased significantly during isradipine treatment; this increase was inversely related to the pretreatment content of serotonin in platelets. The results indicate that treatment with isradipine restores the impaired handling of platelet serotonin as well as the platelet response to serotonin and LDL in hypertensive patients. This effect of isradipine may be regarded as one of the cellular mechanisms of thrombovascular protection and may be of clinical significance in terms of platelet and vessel wall interaction.
- MeSH
- agregace trombocytů účinky léků MeSH
- blokátory kalciových kanálů terapeutické užití MeSH
- dihydropyridiny terapeutické užití MeSH
- dospělí MeSH
- hypertenze krev farmakoterapie MeSH
- inhibitory agregace trombocytů terapeutické užití MeSH
- isradipin MeSH
- krevní tlak účinky léků MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipoproteiny LDL farmakologie MeSH
- senioři MeSH
- serotonin krev farmakologie MeSH
- techniky in vitro MeSH
- trombocyty účinky léků metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- blokátory kalciových kanálů MeSH
- dihydropyridiny MeSH
- inhibitory agregace trombocytů MeSH
- isradipin MeSH
- lipoproteiny LDL MeSH
- serotonin MeSH
