Most cited article - PubMed ID 12422521
Production of manganese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes
Three new chromatographic forms of Dichomitus squalens manganese-dependent peroxidase (MnP) were isolated from wheat-straw cultures using Mono Q and connective interaction media (CIM) fast protein liquid chromatography. Enzymes revealed identical molar mass of 50 kDa (estimated by SDS-PAGE) and pI values of 3.5, however, they varied in Km values obtained for Mn2+ oxidation. The addition of wood and straw methanol extracts to the cultures showed that the production of MnPs in wheat-straw cultures was influenced rather by the type of cultivation than by phenolic compounds from lignocellulosic material which induced laccase production. The purified CIM1 MnP was able to decolorize selected azo and anthraquinone dyes more rapidly than laccase Lc1. In vitro dye decolorization showed a synergistic cooperation of MnP and laccase. In the case of CSB degradation MnP prevented from the production of a differently colored substance that could be produced after CSB degradation by laccase-HBT system.
- MeSH
- Anthraquinones metabolism MeSH
- Azo Compounds metabolism MeSH
- Bacterial Proteins isolation & purification metabolism MeSH
- Color MeSH
- Coloring Agents metabolism MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Laccase isolation & purification metabolism MeSH
- Lignin metabolism MeSH
- Molecular Weight MeSH
- Mycology methods MeSH
- Naphthalenesulfonates metabolism MeSH
- Oxidation-Reduction MeSH
- Peroxidases isolation & purification metabolism MeSH
- Polyporaceae enzymology MeSH
- Triticum MeSH
- Drug Synergism MeSH
- Trypan Blue metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Anthraquinones MeSH
- Azo Compounds MeSH
- Bacterial Proteins MeSH
- Coloring Agents MeSH
- C.I. Reactive Violet 5 MeSH Browser
- Laccase MeSH
- Lignin MeSH
- lignocellulose MeSH Browser
- manganese peroxidase MeSH Browser
- Naphthalenesulfonates MeSH
- Peroxidases MeSH
- pontamine sky blue MeSH Browser
- reactive orange 16 MeSH Browser
- Remazol Brilliant Blue R MeSH Browser
- Trypan Blue MeSH
Conidia production of Beauveria sp. strain LAG by solid-state fermentation (SSF) using blends of agro-industrial residues (residual potatoes and sugar-cane bagasse) was optimized with respect to cultivation conditions and the composition of substrate mixture in Erlenmeyer flasks and column-type bioreactor. With a blend of 60 % residual potatoes and 40 % sugar-cane bagasse the optimum conditions achieved were: incubation temperature 26 degrees C, initial substrate pH 6, inoculum concentration 10(7) conidia per g substrate; optimal initial moisture of the substrate was 70 % for Erlenmeyer flasks, in column-type bioreactor (with forced aeration) the optimal initial moisture of the substrate was 65 % with airflow of 60 mL/min. The highest production (1.07 x 10(10) conidia per g dry substrate) was achieved after a 10-d fermentation. The conidia were used in laboratory assays against Thelosia camina and Hylesia sp., caterpillars that are serious pests of mate plants. The mortality of T. camina was >90 % 10 d after spraying caterpillars with 1 mL conidia suspension at a concentration 10(5)-10(8)/mL. For Hylesia sp., the mortality was 70 %, 7 d after immersion in the conidia suspension containing 108 conidia per mL. Therefore, the Beauveria sp. LAG can be considered to be an important biocontrol instrument in the prospect of the Integrated Pest Management for mate plants.
A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating agents, detergents and cell-wall-hydrolyzing chitinase. Considerable amount of exopolysaccharides from fungal biomass was removed with the use of 1% CTAB wash buffer followed by centrifugation. The DNA varied in terms of yield and quality. For the DNA extraction use of 2% SDS in extraction buffer worked best for Pycnoporus cinnabarinus, Cyathus bulleri, Cyathus striatus and Cyathus stercoreus, while 2% CTAB worked best for Phanerochaete chrysosporium and Pleurotus ostreatus. Elimination of phenol and use of absolute ethanol for precipitating DNA resulted in good yield and quality of DNA. This DNA was amenable to restriction endonuclease digestion.
- MeSH
- Basidiomycota chemistry MeSH
- Cetrimonium Compounds chemistry MeSH
- Cetrimonium MeSH
- Chitinases metabolism MeSH
- Detergents MeSH
- DNA, Fungal analysis isolation & purification metabolism MeSH
- Phenol chemistry MeSH
- Fractional Precipitation MeSH
- Mycology methods MeSH
- Phanerochaete chemistry MeSH
- Pleurotus chemistry MeSH
- Polysaccharides chemistry MeSH
- DNA Restriction Enzymes metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cetrimonium Compounds MeSH
- Cetrimonium MeSH
- Chitinases MeSH
- Detergents MeSH
- DNA, Fungal MeSH
- Phenol MeSH
- Polysaccharides MeSH
- DNA Restriction Enzymes MeSH
Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The highest decrease of absorbance A450 was observed in the growth phase regardless of the presence of inducers Tween 80 or poly(ethylene glycol) (PEG). Fluorescein linked to PEG was prepared and, after addition to cultures, shown to stimulate the production of lignin peroxidase. Passing of fluorescing substances into hyphae (observed by confocal microscopy) showed that they were concentrated on some structures inside hyphae.
- MeSH
- Enzyme Induction MeSH
- Fluorescein chemistry metabolism MeSH
- Fluorescent Dyes chemistry metabolism MeSH
- Hyphae metabolism MeSH
- Culture Media MeSH
- Peroxidases metabolism MeSH
- Phanerochaete growth & development metabolism MeSH
- Polyethylene Glycols chemistry pharmacology MeSH
- Polysorbates pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Fluorescein MeSH
- Fluorescent Dyes MeSH
- Culture Media MeSH
- lignin peroxidase MeSH Browser
- Peroxidases MeSH
- Polyethylene Glycols MeSH
- Polysorbates MeSH
The potential use of fungal pellets for decolorization of the textile dyeing wastewater was evaluated. The live pellets of the fungus Phanerochaete chrysosporium were found to remove more than 95% of the color of this wastewater within 1 d. The dye-removal capacity was a function of time and was proportional to the agitation rate; the optimum temperature was 30 degrees C. Both live and dead pellets were further examined in a repeated-batch mode for 5 d. The decolorization performance of live pellets remained high and stable for 5 d and they showed twice to thrice higher decolorization capacity than dead pellets.
- MeSH
- Biodegradation, Environmental MeSH
- Water Pollutants, Chemical metabolism MeSH
- Indoles metabolism MeSH
- Phanerochaete metabolism MeSH
- Textile Industry methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Water Pollutants, Chemical MeSH
- indirubin MeSH Browser
- Indoles MeSH
The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates of Pleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.
- MeSH
- Enzyme Activation drug effects MeSH
- Azo Compounds metabolism MeSH
- Laccase metabolism MeSH
- Environmental Pollutants metabolism MeSH
- Copper pharmacology MeSH
- Peroxidases metabolism MeSH
- Pleurotus classification enzymology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Azo Compounds MeSH
- Laccase MeSH
- Environmental Pollutants MeSH
- manganese peroxidase MeSH Browser
- Copper MeSH
- Orange G MeSH Browser
- Peroxidases MeSH
Manganese peroxidase (MnP) was produced by shallow stationary cultures of Phanerochaete chrysosporium growing on N-limited medium. Decolorization of sulfonphthalein (SP) dyes by MnP was investigated. The MnP activity profile and decolorization of SP dyes was correlated and almost all dyes were decolorized at pH 4.0. The influence of various inhibitors on Bromocresol Purple decolorization suggested an oxidative nature of the MnP-catalyzed decolorization of SP dyes.
- MeSH
- Enzyme Activation drug effects MeSH
- Sodium Azide pharmacology MeSH
- Coloring Agents metabolism MeSH
- Chelating Agents pharmacology MeSH
- Cysteine pharmacology MeSH
- Edetic Acid pharmacology MeSH
- Phenolphthaleins metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Colorimetry MeSH
- Environmental Pollutants metabolism MeSH
- Peroxidases antagonists & inhibitors metabolism MeSH
- Phanerochaete enzymology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Sodium Azide MeSH
- Coloring Agents MeSH
- Chelating Agents MeSH
- Cysteine MeSH
- Edetic Acid MeSH
- Phenolphthaleins MeSH
- Enzyme Inhibitors MeSH
- Environmental Pollutants MeSH
- manganese peroxidase MeSH Browser
- Peroxidases MeSH
Molasses wastewater (vinasse; the by-product of distillation of fermented sugar) was decolorized and its chemical oxygen demand (COD) was reduced in static cultivation using the fungi Coriolus versicolor, Funalia trogii, Phanerochaete chrysosporium and Pleurotus pulmonarius ('Pleurotus sajorcaju'). The effect of cotton stalk on decolorizing and COD removing capability of four fungi was determined. In the entire concentration range tested (10-30%), wastewater was effectively decolorized by C. versicolor and F. trogii. Cotton stalk addition stimulated the decolorization activity of all fungi. The utilization of cotton stalk represents several advantages due to its function as an attachment place and as a source of nutrients; its use also reduces process costs.
- MeSH
- Color MeSH
- Biodegradation, Environmental * MeSH
- Fermentation MeSH
- Fungi metabolism MeSH
- Molasses * MeSH
- Water Microbiology MeSH
- Waste Disposal, Fluid MeSH
- Pleurotus metabolism MeSH
- Polyporales metabolism MeSH
- Industrial Waste * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Industrial Waste * MeSH
The ability to decolorize four synthetic dyes (Phenol Red, Evans Blue, Eosin Yellowish and Poly B411) in five Pleurotus ostreatus strains (a parental strain and four isolates derived from it) was determined. Two of the isolates had markedly higher and other two substantially lower production of ligninolytic enzymes and hydrogen peroxide than the parental strain. Like the parental strain, the higher-producing isolates were able to decolorize all the tested dyes, but not to a higher extent than the parental strain. In contrast, two lower-producing isolates exhibited slow decolorization, which was incomplete even at the end of cultivation. Evans Blue and Eosin Yellowish strongly suppressed the growth of the strains, while Phenol Red and Poly B411 induced none or only a very slight growth reduction.
- MeSH
- Color MeSH
- Coloring Agents metabolism MeSH
- Laccase MeSH
- Oxidoreductases metabolism MeSH
- Hydrogen Peroxide metabolism MeSH
- Peroxidases metabolism MeSH
- Pleurotus chemistry enzymology metabolism MeSH
- Textile Industry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Coloring Agents MeSH
- Laccase MeSH
- manganese peroxidase MeSH Browser
- Oxidoreductases MeSH
- Hydrogen Peroxide MeSH
- Peroxidases MeSH
