Skin microbiome main cultivable aerobes in human are coagulase-negative staphylococci and lipophilic corynebacteria. Staphylococcus strains (155) belonging to 10 species and 105 strains of Corynebacterium belonging to nine species from the skin swabs of healthy male volunteers were investigated to determine their enzymatic activity to main metabolic substrates: carbohydrates, proteins, lipids, and response to factors present on the skin such as osmotic pressure, pH, and organic acids. The results showed that lipophilic corynebacteria have different capacity for adaptation on the skin than staphylococci. Most of Corynebacterium spp. expressed lack of proteinase, phospholipase, and saccharolytic enzymes activity. Corynebacteria were also more sensitive than Staphylococcus spp. to antimicrobial agents existing on human skin, especially to low pH. These characters can explain domination of Staphylococcus genera on healthy human skin. It can be suggested that within these two bacterial genus, there exists conceivable cooperation and reciprocal protection which results in their quantitative ratio. Such behavior must be considered as crucial for the stability of the population on healthy skin.

• MeSH
• bakteriální proteiny metabolismus   MeSH
• Corynebacterium klasifikace   enzymologie   izolace a purifikace   fyziologie   MeSH
• dospělí MeSH
• kůže mikrobiologie   MeSH
• lidé MeSH
• Staphylococcus klasifikace   enzymologie   izolace a purifikace   fyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální proteiny MeSH

A total 355 of Staphylococcus cohnii isolates from hospital environment, patients (newborns), medical staff and from non-hospital environment were tested for hemolytic activity. Ninety-one % of S. cohnii ssp. cohnii and 74.5 % S. cohnii ssp. urealyticus strains exhibited hemolysis synergistic to S. aureus ATCC 25923 strain. Crude preparations of hemolysins of both bacterial subspecies presented delta-hemolysin, but not alpha- and beta-toxin activity. Highly pure hemolysins were obtained by semipreparative SDS-PAGE or by organic solvent extraction from the freeze-dried crude preparations. Native-PAGE and 2D-PAGE showed their high heterogeneity. Molar masses of single hemolysin units estimated by the Tris-Tricine-SDS-PAGE were calculated as 3.47 kDa for S. cohnii ssp. cohnii and 3.53 kDa for S. cohnii ssp. urealyticus.

• MeSH
• dospělí MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• hemolyziny izolace a purifikace   metabolismus   MeSH
• infekce spojené se zdravotní péčí mikrobiologie   MeSH
• isoelektrická fokusace MeSH
• kůže mikrobiologie   MeSH
• lidé MeSH
• molekulová hmotnost MeSH
• novorozenec MeSH
• personál nemocniční MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus chemie   izolace a purifikace   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Polsko MeSH
• Názvy látek
• hemolyziny MeSH

A total of 5 Bifidobacterium spp. isolated from pig and children' feces and 6 Lactobacillus spp. from chicken feces were examined for expression of aggregation, cell surface hydrophobicity (CSH) and adherence to intestinal mucin. Co-aggregation activity was seen in 3 strains of auto-aggregative bifidobacteria and 4 auto-aggregative strains of Lactobacillus spp. with 2 enterohemorrhagic E. coli (O157). CSH correlated with Lactobacillus auto-aggregating activity and adherence to mucin but the correlation between Bifidobacterium adherence to mucin and CSH was not confirmed.

• MeSH
• bakteriální adheze * MeSH
• Bifidobacterium izolace a purifikace   fyziologie   MeSH
• Escherichia coli O157 fyziologie   MeSH
• feces mikrobiologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• kur domácí mikrobiologie   MeSH
• Lactobacillus izolace a purifikace   fyziologie   MeSH
• lidé MeSH
• muciny metabolismus   MeSH
• prasata mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• muciny MeSH

Numerous isolates of both subspecies of Staphylococcus cohnii were found in the environment of the intensive-care unit of a pediatric hospital. These isolates carried in their cells many plasmids, up to fourteen, of a wide range of sizes (< 2 to > 56 kb). Striking was the occurrence of large plasmids not very common in staphylococci. These were present in > 80% of S. cohnii isolates. Fifty-two different plasmid profiles were found in 79 investigated isolates belonging to S. cohnii ssp. cohnii and S. cohnii ssp. urealyticus. Isolates similar in plasmid profiles were grouped in antibiotic-resistance clusters established for 9 antibiotics (gentamicin, ciprofloxacin, clindamycin, erythromycin, tetracycline, chloramphenicol, mupirocin, trimethoprim-sulfamethoxazole, vancomycin) using the method of unweighted pair group mathematical averages (UPGMA). Many isolates were multiresistant to antibiotics and produced bacteriocins.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence genetika   MeSH
• bakteriociny biosyntéza   MeSH
• chloramfenikol farmakologie   MeSH
• ciprofloxacin farmakologie   MeSH
• dětské nemocnice * MeSH
• DNA bakterií analýza   izolace a purifikace   MeSH
• DNA fingerprinting MeSH
• erythromycin farmakologie   MeSH
• gentamiciny farmakologie   MeSH
• jednotky intenzivní péče * MeSH
• klindamycin farmakologie   MeSH
• kombinace léků trimethoprim a sulfamethoxazol farmakologie   MeSH
• mikrobiální testy citlivosti MeSH
• mnohočetná bakteriální léková rezistence genetika   MeSH
• molekulární epidemiologie MeSH
• mupirocin farmakologie   MeSH
• plazmidy * MeSH
• Staphylococcus klasifikace   účinky léků   genetika   izolace a purifikace   MeSH
• techniky typizace bakterií MeSH
• tetracyklin farmakologie   MeSH
• vankomycin farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Polsko MeSH
• Názvy látek
• antibakteriální látky MeSH
• bakteriociny MeSH
• chloramfenikol MeSH
• ciprofloxacin MeSH
• DNA bakterií MeSH
• erythromycin MeSH
• gentamiciny MeSH
• klindamycin MeSH
• kombinace léků trimethoprim a sulfamethoxazol MeSH
• mupirocin MeSH
• tetracyklin MeSH
• vankomycin MeSH

Serotyping and some potential virulence-associated markers were investigated in Plesiomonas shigelloides strains isolated from humans, animals and aquatic environments. Surface properties of these strains were evaluated using Congo red binding, salt-aggregation test, bacterial adherence to xylene and motility. Production of pancreatic elastase, proteinase (consistent with subtilisin Carlsberg), triacylglycerol lipase, histidine decarboxylase and beta-hemolysin was also determined. In addition, detection of signal molecules such as C4-C8 unsubstituted N-acylhomoserine lactones (AHLs) was performed. The serological typing of the P. shigelloides strains showed that the isolates belonged to 13 different serovars. The majority of the strains were hydrophobic and motile. The strains produced low levels of elastase, proteinase and histidine decarboxylase whereas triacylglycerol lipase activity was relatively high. Only 23.3 % of the strains produced hemolysin. The AHLs signal molecules were not detected. P. shigelloides strains were able to produce a variety of potential virulence markers which may be involved in the pathogenesis of Plesiomonas-associated infections.

• MeSH
• bakteriální adheze MeSH
• druhová specificita MeSH
• gama-butyrolakton analogy a deriváty   metabolismus   MeSH
• gramnegativní bakteriální infekce mikrobiologie   MeSH
• hemolyziny biosyntéza   MeSH
• histidindekarboxylasa biosyntéza   MeSH
• lidé MeSH
• lipasa biosyntéza   MeSH
• mikrobiologie vody MeSH
• pankreatická elastasa biosyntéza   MeSH
• Plesiomonas klasifikace   izolace a purifikace   metabolismus   patogenita   MeSH
• sérotypizace MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• gama-butyrolakton MeSH
• hemolyziny MeSH
• histidindekarboxylasa MeSH
• homoserine lactone MeSH Prohlížeč
• lipasa MeSH
• pankreatická elastasa MeSH

Successful establishment of sepsis by entrapping a dose of 150 colony forming units of Klebsiella pneumoniae in a fibrin clot following implantation into the peritoneal cavity of mice is reported. The dose in the fibrin clot gave 50% mortality in mice, spread over a period of one week. All the infected mice showed positive blood culture up to 6 d post-infection; histopathology revealed inflammatory changes in both liver and spleen. Introduction of K. pneumoniae into experimental mice without entrapment in fibrin clot caused no mortality and blood culture remained positive only up to 2 d; histopathology of liver and spleen throughout the period of study showed relatively mild inflammatory changes, which almost cleared during 14 d post-infection. The use of the fibrin-clot model may thus be considered to be useful in studying both the initial and the persisting stage of infection in the peritoneum, whence a slow release of bacteria into the blood takes place which finally leads to sepsis and septicemia.

• MeSH
• fibrin MeSH
• inbrední kmeny myší * MeSH
• infekce bakteriemi rodu Klebsiella mikrobiologie   mortalita   patologie   MeSH
• játra mikrobiologie   patologie   MeSH
• Klebsiella pneumoniae * MeSH
• modely nemocí na zvířatech * MeSH
• myši MeSH
• peritoneální dutina mikrobiologie   MeSH
• sepse mikrobiologie   mortalita   patologie   MeSH
• slezina mikrobiologie   patologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fibrin MeSH

The prevalence of extended-spectrum beta-lactamases (ESBL) was determined among isolates of Escherichia coli (n = 63) isolated from hospitalized (43) and healthy (20) children. Ten isolates (21%) were ESBL-positive for two screening tests, the double disk-synergy test and the Oxoid Combination Disk method. One ESBL-positive isolate came from a healthy child. The transfer frequency of oxyimino-beta-lactam resistance from ESBL-producing isolates to E. coli K12 C600 recipient strain ranged from 10(-8) to 10(-5) per donor cell. Donor strains and transconjugants displayed susceptibility patterns typical of ESBL producers. They were resistant to oxyimino-beta-lactams but susceptible to clavulanic acid and carbapenems. Seven out of the 10 ESBL-positive isolates were found to produce MR/MS fimbria, which may play an important role in the colonization of the human intestinal mucosa.

• MeSH
• beta-laktamasy metabolismus   MeSH
• beta-laktamová rezistence MeSH
• beta-laktamy farmakologie   MeSH
• dítě MeSH
• Escherichia coli klasifikace   enzymologie   izolace a purifikace   MeSH
• hospitalizace * MeSH
• infekce vyvolané Escherichia coli epidemiologie   mikrobiologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• předškolní dítě MeSH
• průjem epidemiologie   mikrobiologie   MeSH
• střevní sliznice mikrobiologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• beta-laktamasy MeSH
• beta-laktamy MeSH