We evaluated the suitability of various primers for the RAPD (random amplified polymorphic DNA) accurate species identification and strain typing of Aspergillus clinical isolates. Five primers described previously were tested for their discriminatory power in three Aspergillus species (A. fumigatus, A. niger agg. and A. flavus - 23 clinical isolates and 2 reference strains). Clustering of RAPD fingerprints corresponded well with the identification based on morphological features. All isolates were resolved as different strains using the primer R108 and the RAPD protocol optimized for a Robocycler thermal cycler. RAPD with the primer R108 thus can be considered to be a valuable, simple and powerful tool for identification and strain delineation of Aspergillus spp.

• MeSH
• Aspergillus flavus classification   MeSH
• Aspergillus fumigatus classification   MeSH
• Aspergillus niger classification   MeSH
• Aspergillosis diagnosis   microbiology   MeSH
• DNA Fingerprinting methods   MeSH
• DNA Primers MeSH
• Humans MeSH
• Mycological Typing Techniques methods   MeSH
• Reproducibility of Results MeSH
• Random Amplified Polymorphic DNA Technique methods   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Evaluation Study MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA Primers MeSH

The relationship between inflammatory bowel disease and microorganisms was evaluated. The presence of Candida albicans-specific IgM and IgG antibodies in serum samples and the presence of C. albicans in stool and colonal mucosa samples of the patients did not exhibit any significant difference between 21 patients in active stage and 15 patients in remission of ulcerative colitis (UC) (compared with 19 control patients). The invasion of yeast cells to the colonal mucosa was demonstrated by detecting C. albicans DNA using specific PCon1, PCon2, and PspA2 primers in PCR assay. Eighteen of 36 patients (50%) were found to be DNA positive while in 19 controls only 4 (21%) were found to be positive. The presence of DNA in the association of the positive serological reactivity is suggested as an important diagnostic marker of UC.

• MeSH
• Models, Biological MeSH
• Candida albicans genetics   immunology   isolation & purification   pathogenicity   MeSH
• DNA, Fungal analysis   genetics   MeSH
• Feces microbiology   MeSH
• Immunoglobulin G blood   MeSH
• Immunoglobulin M blood   MeSH
• Humans MeSH
• Polymerase Chain Reaction MeSH
• Antibodies, Fungal blood   MeSH
• Base Sequence MeSH
• Intestinal Mucosa microbiology   MeSH
• Case-Control Studies MeSH
• Colitis, Ulcerative etiology   immunology   microbiology   pathology   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• DNA, Fungal MeSH
• Immunoglobulin G MeSH
• Immunoglobulin M MeSH
• Antibodies, Fungal MeSH

Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.

• MeSH
• Aspartic Acid Endopeptidases analysis   genetics   MeSH
• Candida albicans enzymology   genetics   isolation & purification   MeSH
• DNA, Fungal analysis   MeSH
• Fungal Proteins analysis   genetics   MeSH
• Genes, Fungal MeSH
• Isoenzymes antagonists & inhibitors   genetics   MeSH
• Candidiasis microbiology   MeSH
• Blood microbiology   MeSH
• Humans MeSH
• Polymerase Chain Reaction methods   MeSH
• Vagina microbiology   MeSH
• Check Tag
• Humans MeSH
• Male MeSH
• Female MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Aspartic Acid Endopeptidases MeSH
• DNA, Fungal MeSH
• Fungal Proteins MeSH
• Isoenzymes MeSH
• SAP1 protein, Candida albicans MeSH Browser
• SAP2 protein, Candida MeSH Browser
• SAP3 protein, Candida albicans MeSH Browser
• SAP4 protein, Candida albicans MeSH Browser
• SAP5 protein, Candida albicans MeSH Browser