Cyanobacterial pigments have attracted considerable attention in industry due to their bioactive potential and natural origin. In the present study, the growth dynamics and pigment composition, in terms of chlorophyll a, total carotenoids and phycobiliprotein content, of four cyanobacterial strains isolated from thermal springs, namely Oscillatoria subbrevis CZS 2201, Phormidium ambiguum CZS 2205, Nostoc calcicola TSZ 2203, and Synechococcus sp. CZS 2204, were investigated. The analysis revealed that the maximum quantity of chlorophyll a and total carotenoids was observed in Oscillatoria subbrevis CZS 2201 (26.49 and 3.44 µg mL-1), followed by Phormidium ambiguum CZS 2205 (18.64 and 2.32 µg mL-1), whereas a minimum amount was detected in Synechococcus sp. CZS 2204 (12.13 and 1.24 µg mL-1), respectively. In addition, Oscillatoria subbrevis CZS 2201 showed higher quantity of phycobiliproteins, especially C-phycocyanin (45.81 mg g-1), C-phycoerythrin (64.17 mg g-1) and C-allophycocyanin (27.45 mg g-1). Moreover, carotenoid derivatives of Oscillatoria subbrevis CZS 2201 were also identified, among which β-carotene was the dominant form (1.94 µg mL-1), while the accumulation of zeaxanthin and myxoxanthophyll was relatively high (0.53 and 0.41 µg mL-1, respectively) compared with echinenone and cryptoxanthin (0.34 and 0.23 µg mL-1, respectively). The study revealed that Oscillatoria subbrevis CZS 2201 was a potent producer of secondary carotenoids, including myxoxanthophyll.

• Klíčová slova
• biological activities, carotenoids, chromatography, cyanobacteria, extraction of pigments, identification, myxoxanthophyll,
• Publikační typ
• časopisecké články MeSH

Nitrogen is one of the most important nutrient sources for the growth of microalgae. We studied the effects of nitrogen starvation on the growth responses, biochemical composition, and fatty acid profile of Dunaliella tertiolecta, Phaeodactylum tricornutum, and Nannochloropsis oculata. The lack of nitrogen caused changes in carbohydrate, protein, lipid, and fatty acid composition in all examined microalgae. The carbohydrate content increased 59% in D. tertiolecta, while the lipid level increased 139% in P. tricornutum under nitrogen stress conditions compared to the control groups. Nitrogen starvation increased the oligosaccharide and polysaccharide contents of D. tertiolecta 4.1-fold and 3.6-fold, respectively. Furthermore, triacylglycerol (TAG) levels in N. oculata and P. tricornutum increased 2.3-fold and 7.4-fold, respectively. The dramatic increase in the amount of TAG is important for the use of these microalgae as raw materials in biodiesel. Nitrogen starvation increased the amounts of oligosaccharides and polysaccharides of D. tertiolecta, while increased eicosapentaenoic acid (EPA) in N. oculata and docosahexaenoic acid (DHA) content in P. tricornutum. The amount of polyunsaturated fatty acids (PUFAs), EPA, DHA, oligosaccharides, and polysaccharides in microalgal species can be increased without using the too costly nitrogen source in the culture conditions, which can reduce the most costly of living feeding.

• Klíčová slova
• Biochemical composition, Fatty acid profile, Microalgae, Nitrogen starvation,
• MeSH
• dusík * metabolismus   MeSH
• mastné kyseliny * metabolismus   analýza   MeSH
• mikrořasy * metabolismus   růst a vývoj   MeSH
• triglyceridy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• dusík * MeSH
• mastné kyseliny * MeSH
• triglyceridy MeSH

Light is the essential energy source for autotrophically growing organisms, including microalgae. Both light intensity and light quality affect cell growth and biomass composition. Here we used three green algae-Chlamydomonas reinhardtii, Desmodesmus quadricauda, and Parachlorella kessleri-to study the effects of different light intensities and light spectra on their growth. Cultures were grown at three different light intensities (100, 250, and 500 µmol m-2 s-1) and three different light sources: fluorescent lamps, RGB LEDs, and white LEDs. Cultures of Desmodesmus quadricauda and Parachlorella kessleri were saturated at 250 µmol m-2 s-1, and further increasing the light intensity did not improve their growth. Chlamydomonas reinhardtii cultures did not reach saturation under the conditions used. All species usually divide into more than two daughter cells by a mechanism called multiple fission. Increasing light intensity resulted in an increase in maximum cell size and division into more daughter cells. In Parachlorella kessleri cells, the concentration of photosynthetic pigments decreased with light intensity. Different light sources had no effect on algal growth or photosynthetic pigments. The results show a species-specific response of algae to light intensity and support the use of any white light source for their cultivation without negative effects on growth.

• Klíčová slova
• Chlamydomonas reinhardtii, Desmodesmus quadricauda, LED, Parachlorella kessleri, cell growth, fluorescent tube, light intensity,
• MeSH
• biomasa MeSH
• Chlamydomonas reinhardtii * MeSH
• Chlorophyta * MeSH
• fotosyntéza MeSH
• mikrořasy * MeSH
• světlo MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Milk fat is an important nutritional compound in the human diet. From the health point of view, some fatty acids (FAs), particularly long-chain PUFAs such as EPA and DHA, have been at the forefront of interest due to their antibacterial, antiviral, anti-inflammatory, and anti-tumor properties, which play a positive role in the prevention of cardiovascular diseases (CVD), as well as linoleic and γ-linolenic acids, which play an important role in CVD treatment as essential components of phospholipids in the mitochondria of cell membranes. Thus, the modification of the FA profile-especially an increase in the concentration of polyunsaturated FAs and n-3 FAs in bovine milk fat-is desirable. The most effective way to achieve this goal is via dietary manipulations. The effects of various strategies in dairy nutrition have been thoroughly investigated; however, there are some alternative or unconventional feedstuffs that are often used for purposes other than basic feeding or modifying the fatty acid profiles of milk, such as tanniferous plants, herbs and spices, and algae. The use of these foods in dairy diets and their effects on milk fatty acid profile are reviewed in this article. The contents of selected individual FAs (atherogenic, rumenic, linoleic, α-linolenic, eicosapentaenoic, and docosahexaenoic acids) and their combinations; the contents of n3 and n6 FAs; n6/n3 ratios; and atherogenic, health-promoting and S/P indices were used as criteria for assessing the effect of these feeds on the health properties of milk fat.

• Klíčová slova
• algae, camelina, dairy cows, health, herbs and spices, indices, milk fat quality, okara, tannins,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

This study focused on supplementing pikeperch (Sander lucioperca) larvae with rotifers fed with Chlorella vulgaris during the first 15 days post hatching (dph). Larvae were fed a combination of rotifers and artemia under three different enrichments: A) Nannochloropsis occulata, B) Chlorella vulgaris, and C) a commercial enrichment-Selco, Spresso from INVE. After 17 days from the trial initiation differences were found between treatments on survival rate, myomere height (MH), fatty acid composition, and stress tolerance. In terms of survival, larvae from treatment b (74.5%) and c (66%) excelled over the control (a) treatment (59%). Furthermore, larvae from both the Chlorella (b) and the Selco (c) treatments showed more resilience to stress conditions (10% and 37% reduction in mortality) when exposed to high salinity conditions (18ppt) for 3 h (stress response). Overall, larvae from treatments b and c performed better than those receiving a non-enriched diet (a), likely due to the higher levels of Essential Fatty Acids (EFA) and the ability of pikeperch to desaturate and elongate fatty acids (FA) with 18 carbons to LC PUFAs (Polyunsaturated Fatty Acids). The present study provides valuable input for designing improved feeding protocols, which will increase the efficiency of pikeperch larval culture.

• Publikační typ
• časopisecké články MeSH