Inflammatory or anti-inflammatory? That is the question as far as the acute-phase response and its mediators, the pentraxins, are concerned. Only some ten years ago, the classical or short pentraxin C-reactive protein and the newly discovered long pentraxin PTX3 were considered to exert most of the detrimental effects of acute inflammation, whether microbial or sterile in origin. However, accumulating evidence suggests an at least dichotomous, context-dependent outcome attributable to the pentraxins, if not a straightforward anti-inflammatory nature of the acute-phase response. This paper is focused on the inherent effects of pentraxin 3 in inflammatory responses, mainly in coronary artery disease and in Aspergillus fumigatus infection. Both are examples of inflammatory reactions in which PTX3 is substantially involved; the former sterile, the latter infectious in origin. Apart from different inducing noxae, similarities in the pathogenesis of the two are striking. All the same, the introductory question still persists: is the ultimate impact of PTX3 in these conditions inflammatory or anti-inflammatory, paradoxical as the latter might appear? We try to provide an answer such as it emerges in the light of recent findings.

• MeSH
• Aspergillus fumigatus metabolismus   MeSH
• ateroskleróza metabolismus   MeSH
• C-reaktivní protein genetika   fyziologie   MeSH
• infarkt myokardu metabolismus   MeSH
• kardiovaskulární nemoci krev   metabolismus   MeSH
• komplement MeSH
• lidé MeSH
• myši MeSH
• neutrofily metabolismus   MeSH
• přirozená imunita MeSH
• reperfuzní poškození MeSH
• sérový amyloidový protein genetika   fyziologie   MeSH
• zánět MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• C-reaktivní protein MeSH
• komplement MeSH
• PTX3 protein MeSH Prohlížeč
• sérový amyloidový protein MeSH

OBJECTIVE: Activation of innate immunity cells is inseparably linked to cardiac surgical operation. The aim of this study was to assess the kinetics in the expression of receptor for Fc part of IgG, FcgammaRI (CD64), and scavenger receptor CD163 on peripheral blood cells of cardiac surgical patients and to examine the effect of cardiac bypass as a separable influence on the systemic acute inflammatory response. METHODS: Forty patients, twenty in each group, were randomly assigned to CABG surgery performed either with "on-pump" or without "off-pump" cardiopulmonary bypass. Standardized quantitative flow cytometry method was used to determine the expression of surface markers. RESULTS: The density of CD64 molecule on monocytes reached maximum on the 1st postoperative day (P<.001) whereas the peak for CD64 molecule expression on granulocytes was postponed to the 3rd postoperative day (P<.001). The expression of CD163 scavenger molecule on monocytes reached maximum on the 1st postoperative day (P<.001). The density of CD163 molecule on monocytes on the 1st postoperative day is significantly higher in "on-pump" patients in comparison with "off-pump" patients (P<.001). CONCLUSION: In cardiac surgical patients the expression of activation marker FcgammaR1 (CD64) on monocytes is increased earlier in comparison with granulocytes in both "on-pump" and "off-pump" patients. The expression of scavenger molecule CD163 on monocytes is significantly higher in "on-pump" patients.

• MeSH
• antigen CD163 MeSH
• antigeny diferenciační myelomonocytární krev   MeSH
• CD antigeny krev   MeSH
• infuzní pumpy MeSH
• kardiopulmonální bypass metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• monocyty imunologie   MeSH
• nemoci srdce krev   chirurgie   MeSH
• průtoková cytometrie MeSH
• receptory buněčného povrchu krev   MeSH
• receptory IgG krev   MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• antigen CD163 MeSH
• antigeny diferenciační myelomonocytární MeSH
• CD antigeny MeSH
• receptory buněčného povrchu MeSH
• receptory IgG MeSH

OBJECTIVES: The changes in the serum levels of lipopolysaccharide binding protein (LBP) and sCD14 during cardiac surgery were followed in this study. DESIGN: Thirty-four patients, 17 in each group, were randomly assigned to coronary artery bypass grafting surgery performed either with ("on-pump") or without ("off-pump") cardiopulmonary bypass. LBP and sCD14 were evaluated by ELISA. RESULTS: The serum levels of LBP were gradually increased from the 1st postoperative day and reached their maximum on the 3rd postoperative day in both "on-pump" and "off-pump" patients (30.33+/-9.96 microg/mL; 37.99+/-16.58 microg/mL), respectively. There were no significant differences between "on-pump" and "off-pump" patients regarding LBP. The significantly increased levels of sCD14 from the 1st up to the 7th postoperative day in both "on-pump" and "off-pump" patients were found with no significant differences between these groups. No correlations between LBP and sCD14 and IL-6, CRP and long pentraxin PTX3 levels were found. CONCLUSIONS: The levels of LBP and sCD14 are elevated in cardiac surgical patients being similar in both groups. These molecules are not produced as acute phase proteins in these patients.

• MeSH
• biologické modely MeSH
• C-reaktivní protein metabolismus   MeSH
• časové faktory MeSH
• ELISA MeSH
• interleukin-6 metabolismus   MeSH
• kardiopulmonální bypass škodlivé účinky   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lipopolysacharidové receptory krev   chemie   MeSH
• membránové glykoproteiny metabolismus   MeSH
• proteiny akutní fáze metabolismus   MeSH
• senioři MeSH
• sérový amyloidový protein metabolismus   MeSH
• statistické modely MeSH
• transportní proteiny metabolismus   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• C-reaktivní protein MeSH
• interleukin-6 MeSH
• lipopolysaccharide-binding protein MeSH Prohlížeč
• lipopolysacharidové receptory MeSH
• membránové glykoproteiny MeSH
• proteiny akutní fáze MeSH
• PTX3 protein MeSH Prohlížeč
• sérový amyloidový protein MeSH
• transportní proteiny MeSH