Chlorhexidine (CHX) and octenidine (OCT), antimicrobial compounds used in oral care products (toothpastes and mouthwashes), were recently revealed to interfere with human sex hormone receptor pathways. Experiments employing model organisms-white-rot fungi Irpex lacteus and Pleurotus ostreatus-were carried out in order to investigate the biodegradability of these endocrine-disrupting compounds and the capability of the fungi and their extracellular enzyme apparatuses to biodegrade CHX and OCT. Up to 70% ± 6% of CHX was eliminated in comparison with a heat-killed control after 21 days of in vivo incubation. An additional in vitro experiment confirmed manganese-dependent peroxidase and laccase are partially responsible for the removal of CHX. Up to 48% ± 7% of OCT was removed in the same in vivo experiment, but the strong sorption of OCT on fungal biomass prevented a clear evaluation of the involvement of the fungi or extracellular enzymes. On the other hand, metabolites indicating the enzymatic transformation of both CHX and OCT were detected and their chemical structures were proposed by means of liquid chromatography-mass spectrometry. Complete biodegradation by the ligninolytic fungi was not achieved for any of the studied analytes, which emphasizes their recalcitrant character with low possibility to be removed from the environment.

• Klíčová slova
• chlorhexidine, dental hygiene, laccase, ligninolytic fungi, manganese-dependent peroxidase, octenidine, personal care products, quaternary ammonium compounds, recalcitrant pollutant,
• MeSH
• antiinfekční látky lokální metabolismus   MeSH
• biodegradace * MeSH
• chlorhexidin chemie   metabolismus   MeSH
• houby metabolismus   MeSH
• iminy MeSH
• lidé MeSH
• metabolomika metody   MeSH
• pyridiny chemie   metabolismus   MeSH
• stomatologická péče MeSH
• transformace genetická MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antiinfekční látky lokální MeSH
• chlorhexidin MeSH
• iminy MeSH
• octenidine MeSH Prohlížeč
• pyridiny MeSH

Secondary plant metabolites (SPMEs) play an important role in plant survival in the environment and serve to establish ecological relationships between plants and other organisms. Communication between plants and microorganisms via SPMEs contained in root exudates or derived from litter decomposition is an example of this phenomenon. In this review, the general aspects of rhizodeposition together with the significance of terpenes and phenolic compounds are discussed in detail. We focus specifically on the effect of SPMEs on microbial community structure and metabolic activity in environments contaminated by polychlorinated biphenyls (PCBs) and polyaromatic hydrocarbons (PAHs). Furthermore, a section is devoted to a complex effect of plants and/or their metabolites contained in litter on bioremediation of contaminated sites. New insights are introduced from a study evaluating the effects of SPMEs derived during decomposition of grapefruit peel, lemon peel, and pears on bacterial communities and their ability to degrade PCBs in a long-term contaminated soil. The presented review supports the "secondary compound hypothesis" and demonstrates the potential of SPMEs for increasing the effectiveness of bioremediation processes.

• Klíčová slova
• bioremediation, carbon flow, community structure, secondary plant metabolites (SPMEs),
• MeSH
• Bacteria klasifikace   izolace a purifikace   metabolismus   MeSH
• biodegradace * MeSH
• látky znečišťující půdu chemie   toxicita   MeSH
• polychlorované bifenyly toxicita   MeSH
• půdní mikrobiologie * MeSH
• rostliny metabolismus   mikrobiologie   MeSH
• sekundární metabolismus MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• látky znečišťující půdu MeSH
• polychlorované bifenyly MeSH

Seventy-nine white rot strains were screened to determine if they had the potential for use in the degradation of oligocyclic aromates (PAHs) by measuring their dye-decoloration rate. Fourteen strains that were selected based on their dye-decoloration rate were then evaluated for the ability to tolerate various levels of PAHs spiked in agar medium. The ability of white rot fungi to degrade 3- or 4-ring PAHs (anthracene, phenanthrene, fluoranthene, pyrene) was determined. Two strains of Phanerochaete sordida (KUC8369, KUC8370) were possible PAHs degraders, degrading a significantly greater amount of phenanthrene and fluoranthene than the culture collection strain P. chrysosporium (a known PAHs degrader). The production of manganese peroxidase, the only extracellular ligninolytic enzyme detected during the cultivation, was evaluated.

• MeSH
• barvicí látky MeSH
• Basidiomycota klasifikace   genetika   růst a vývoj   metabolismus   MeSH
• biodegradace MeSH
• DNA fungální analýza   genetika   MeSH
• fenantreny metabolismus   MeSH
• fluoreny metabolismus   MeSH
• kultivační média MeSH
• mezerníky ribozomální DNA analýza   genetika   MeSH
• molekulární sekvence - údaje MeSH
• peroxidasy metabolismus   MeSH
• Phanerochaete klasifikace   genetika   růst a vývoj   metabolismus   MeSH
• polycyklické aromatické uhlovodíky metabolismus   MeSH
• sekvenční analýza DNA MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• barvicí látky MeSH
• DNA fungální MeSH
• fenantreny MeSH
• fluoranthene MeSH Prohlížeč
• fluoreny MeSH
• kultivační média MeSH
• manganese peroxidase MeSH Prohlížeč
• mezerníky ribozomální DNA MeSH
• peroxidasy MeSH
• phenanthrene MeSH Prohlížeč
• polycyklické aromatické uhlovodíky MeSH

White-rot fungi that are efficient lignin degraders responsible for its turnover in nature have appeared twice in the center of biotechnological research - first, when the lignin degradation process started being systematically investigated and major enzyme activities and mechanisms involved were described, and second, when the huge remediation potential of these organisms was established. Originally, Phanerochaete chrysosporium became a model organism, characterized by a secondary metabolism regulatory pattern triggered by nutrient (mostly nitrogen) limitation. Last decade brought evidence of more varied regulatory patterns in white-rot fungi when ligninolytic enzymes were also abundantly synthesized under conditions of nitrogen sufficiency. Gradually, research was focused on other species, among them Irpex lacteus showing a remarkable pollutant toxicity resistance and biodegradation efficiency. Systematic research has built up knowledge of biochemistry and biotechnological applicability of this fungus, stressing the need to critically summarize and estimate these scattered data. The review attempts to evaluate the information on I. lacteus focusing on various enzyme activities and bioremediation of organopollutants in water and soil environments, with the aim of mediating this knowledge to a broader microbiological audience.

• MeSH
• Basidiomycota enzymologie   genetika   metabolismus   MeSH
• biodegradace MeSH
• biotechnologie * MeSH
• fungální proteiny genetika   metabolismus   MeSH
• látky znečišťující životní prostředí metabolismus   MeSH
• lignin metabolismus   MeSH
• regulace genové exprese u hub MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• fungální proteiny MeSH
• látky znečišťující životní prostředí MeSH
• lignin MeSH

The wood-decomposing fungal species Antrodia macra, A. pulvinascens, Ceriporiopsis aneirina, C. resinascens and Dichomitus albidofuscus were determined for production of laccase (LAC), Mn peroxidase (MnP), lignin peroxidase (LiP), endo-l,4-P-beta-glucanase, endo-l,4-beta-xylanase, cellobiohydrolase, 1,4-beta-glucosidase and 1,4-beta-xylosidase. The results confirmed the brown-rot mode of Antrodia spp. which did not produce the activity of LAC and MnP. The remaining species performed detectable activity of both enzymes while no strain produced LiP. Significant inhibition of LAC production by high nitrogen was found in all white-rot species while only MnP of D. albidofuscus was regulated in the same way. The endoglucanase and endoxylanase activities of white-rotting species were inhibited by glucose in the medium while those of Antrodia spp. were not influenced by glucose concentration. The regulation of enzyme activity and bio-mass production can vary even within a single fungal genus.

• MeSH
• Basidiomycota enzymologie   izolace a purifikace   metabolismus   MeSH
• dřevo mikrobiologie   MeSH
• dusík metabolismus   MeSH
• fungální proteiny metabolismus   MeSH
• lakasa genetika   metabolismus   MeSH
• lignin metabolismus   MeSH
• peroxidasy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dusík MeSH
• fungální proteiny MeSH
• lakasa MeSH
• lignin MeSH
• lignocellulose MeSH Prohlížeč
• manganese peroxidase MeSH Prohlížeč
• peroxidasy MeSH

Thirty wood-rotting basidiomycetes, most of them causing white rot in wood, were isolated from fruiting bodies growing on decaying wood from the Sierra de Ayllón (Spain). The fungi were identified on the basis of their morphological characteristics and compared for their ability to decolorize Reactive Black 5 and Reactive Blue 38 (as model of azo and phthalocyanine type dyes, respectively) at 75 and 150 mg/L. Only eighteen fungal strains were able to grow on agar plates in the presence of the dyes and only three species (Calocera cornea, Lopharia spadicea, Polyporus alveolaris) decolorized efficiently both dyes at both concentrations. The ligninolytic activities, involved in decolorization dyes (laccases, lignin peroxidases, Mn-oxidizing peroxidases), were followed in glucose basal medium in the presence of enzyme inducers. The results indicate a high variability of the ligninolytic system within white-rot basidiomycetes. These fungal species and their enzymes can represent new alternatives for the study of new biological systems to degrade aromatic compounds causing environmental problems.

• MeSH
• barvicí látky metabolismus   MeSH
• Basidiomycota klasifikace   enzymologie   izolace a purifikace   metabolismus   MeSH
• biodegradace MeSH
• dřevo mikrobiologie   MeSH
• fungální proteiny metabolismus   MeSH
• fylogeneze MeSH
• lakasa metabolismus   MeSH
• peroxidasy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• barvicí látky MeSH
• fungální proteiny MeSH
• lakasa MeSH
• lignin peroxidase MeSH Prohlížeč
• peroxidasy MeSH

Three new chromatographic forms of Dichomitus squalens manganese-dependent peroxidase (MnP) were isolated from wheat-straw cultures using Mono Q and connective interaction media (CIM) fast protein liquid chromatography. Enzymes revealed identical molar mass of 50 kDa (estimated by SDS-PAGE) and pI values of 3.5, however, they varied in Km values obtained for Mn2+ oxidation. The addition of wood and straw methanol extracts to the cultures showed that the production of MnPs in wheat-straw cultures was influenced rather by the type of cultivation than by phenolic compounds from lignocellulosic material which induced laccase production. The purified CIM1 MnP was able to decolorize selected azo and anthraquinone dyes more rapidly than laccase Lc1. In vitro dye decolorization showed a synergistic cooperation of MnP and laccase. In the case of CSB degradation MnP prevented from the production of a differently colored substance that could be produced after CSB degradation by laccase-HBT system.

• MeSH
• anthrachinony metabolismus   MeSH
• azosloučeniny metabolismus   MeSH
• bakteriální proteiny izolace a purifikace   metabolismus   MeSH
• barva MeSH
• barvicí látky metabolismus   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• lakasa izolace a purifikace   metabolismus   MeSH
• lignin metabolismus   MeSH
• molekulová hmotnost MeSH
• mykologie metody   MeSH
• naftalensulfonany metabolismus   MeSH
• oxidace-redukce MeSH
• peroxidasy izolace a purifikace   metabolismus   MeSH
• Polyporaceae enzymologie   MeSH
• pšenice MeSH
• synergismus léků MeSH
• trypanová modř metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• anthrachinony MeSH
• azosloučeniny MeSH
• bakteriální proteiny MeSH
• barvicí látky MeSH
• C.I. Reactive Violet 5 MeSH Prohlížeč
• lakasa MeSH
• lignin MeSH
• lignocellulose MeSH Prohlížeč
• manganese peroxidase MeSH Prohlížeč
• naftalensulfonany MeSH
• peroxidasy MeSH
• pontamine sky blue MeSH Prohlížeč
• reactive orange 16 MeSH Prohlížeč
• Remazol Brilliant Blue R MeSH Prohlížeč
• trypanová modř MeSH