In electrospun scaffolds, coaxial electrospinning is gaining increased attention due to its potential for biocomponent encapsulation and controlled delivery. However, the encapsulation of biocomponents, such as liposomes, remains challenging because of their low stability in commonly used electrospinning solvents. This study, therefore, aims to develop a novel coaxial electrospinning formulation for crafting a liposome-encapsulated, rapid-release coaxial fiber. Liposomes demonstrated desirable stability in fish gelatin/phosphate-buffered saline (PBS) solutions, which remain liquid at room temperature and exhibit exceptional spinnability at concentrations exceeding 80 w/v% due to the reduction in surface tension. Fluorescent labelling examinations confirmed the successful encapsulation of liposomes within coaxial fibers electrospun from a 160 w/v% gelatin/PBS core and a 20 w/v% PCL/chloroform/N,N-dimethylformamide (DMF) shell. The gelatin/PBS core solution formed solid ends at the tips of the core-shell fiber post-spinning, while maintaining a liquid state within the shell, thereby enabling the encapsulation of liposomes within the PCL coaxial fiber. Upon exposure to medium, the solid ends dissolve, enabling the rapid release of liposomes. The successful development of this liposome-loaded electrospun coaxial fiber, using fish gelatin, highlights its potential for creating advanced liposome delivery systems.

• Klíčová slova
• PCL, coaxial electrospinning, fish gelatin, liposomes,
• Publikační typ
• časopisecké články MeSH

Electrospun hybrid nanofibers, based on functional agents immobilized in polymeric matrix, possess a unique combination of collective properties. These are beneficial for a wide range of applications, which include theranostics, filtration, catalysis, and tissue engineering, among others. The combination of functional agents in a nanofiber matrix offer accessibility to multifunctional nanocompartments with significantly improved mechanical, electrical, and chemical properties, along with better biocompatibility and biodegradability. This review summarizes recent work performed for the fabrication, characterization, and optimization of different hybrid nanofibers containing varieties of functional agents, such as laser ablated inorganic nanoparticles (NPs), which include, for instance, gold nanoparticles (Au NPs) and titanium nitride nanoparticles (TiNPs), perovskites, drugs, growth factors, and smart, inorganic polymers. Biocompatible and biodegradable polymers such as chitosan, cellulose, and polycaprolactone are very promising macromolecules as a nanofiber matrix for immobilizing such functional agents. The assimilation of such polymeric matrices with functional agents that possess wide varieties of characteristics require a modified approach towards electrospinning techniques such as coelectrospinning and template spinning. Additional focus within this review is devoted to the state of the art for the implementations of these approaches as viable options for the achievement of multifunctional hybrid nanofibers. Finally, recent advances and challenges, in particular, mass fabrication and prospects of hybrid nanofibers for tissue engineering and biomedical applications have been summarized.

• Klíčová slova
• bone regeneration, drug delivery, electrospinning, functional agents, hybrid nanofibers, nanomedicine, nanoparticles, tissue engineering,
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

Additive manufacturing, also called 3D printing, is an effective method for preparing scaffolds with defined structure and porosity. The disadvantage of the technique is the excessive smoothness of the printed fibers, which does not support cell adhesion. In the present study, a 3D printed scaffold was combined with electrospun classic or structured nanofibers to promote cell adhesion. Structured nanofibers were used to improve the infiltration of cells into the scaffold. Electrospun layers were connected to 3D printed fibers by gluing, thus enabling the fabrication of scaffolds with unlimited thickness. The composite 3D printed/nanofibrous scaffolds were seeded with primary chondrocytes and tested in vitro for cell adhesion, proliferation and differentiation. The experiment showed excellent cell infiltration, viability, and good cell proliferation. On the other hand, partial chondrocyte dedifferentiation was shown. Other materials supporting chondrogenic differentiation will be investigated in future studies.

• Klíčová slova
• 3D printing, cell infiltration, chondrocytes, electrospinning, nanofibres,
• MeSH
• 3D tisk * MeSH
• buněčná adheze fyziologie   MeSH
• buněčná diferenciace fyziologie   MeSH
• chondrocyty cytologie   MeSH
• kultivované buňky fyziologie   MeSH
• lidé MeSH
• nanovlákna * chemie   MeSH
• proliferace buněk fyziologie   MeSH
• tkáňové inženýrství metody   MeSH
• tkáňové podpůrné struktury * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

In this study, we have developed a combined approach to accelerate the proliferation of mesenchymal stem cells (MSCs) in vitro, using a new nanofibrous scaffold made by needleless electrospinning from a mixture of poly-ε-caprolactone and magnetic particles. The biological characteristics of porcine MSCs were investigated while cultured in vitro on composite scaffold enriched with magnetic nanoparticles. Our data indicate that due to the synergic effect of the poly-ε-caprolactone nanofibers and magnetic particles, cellular adhesion and proliferation of MSCs is enhanced and osteogenic differentiation is supported. The cellular and physical attributes make this new scaffold very promising for the acceleration of efficient MSC proliferation and regeneration of hard tissues.

• Klíčová slova
• magnetic particles, mesenchymal stem cells, nanofibers, tissue engineering,
• MeSH
• biokompatibilní materiály chemie   farmakologie   MeSH
• buněčná adheze účinky léků   MeSH
• buněčná diferenciace účinky léků   MeSH
• kapronáty chemie   farmakologie   MeSH
• laktony chemie   farmakologie   MeSH
• magnetické nanočástice chemie   MeSH
• mezenchymální kmenové buňky cytologie   účinky léků   MeSH
• nanovlákna chemie   MeSH
• polyestery farmakologie   MeSH
• prasata MeSH
• proliferace buněk účinky léků   MeSH
• tkáňové inženýrství MeSH
• tkáňové podpůrné struktury chemie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biokompatibilní materiály MeSH
• caprolactone MeSH Prohlížeč
• kapronáty MeSH
• laktony MeSH
• magnetické nanočástice MeSH
• polyestery MeSH

Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

• Klíčová slova
• growth factors, hernia regeneration, in vitro, nanofibers, polypropylene mesh,
• MeSH
• biokompatibilní materiály * chemie   toxicita   MeSH
• chirurgické síťky * MeSH
• incizní kýla chirurgie   MeSH
• myši MeSH
• nanovlákna * chemie   toxicita   MeSH
• polyestery * chemie   toxicita   MeSH
• polypropyleny * chemie   toxicita   MeSH
• proliferace buněk účinky léků   MeSH
• trombocyty cytologie   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biokompatibilní materiály * MeSH
• polycaprolactone MeSH Prohlížeč
• polyestery * MeSH
• polypropyleny * MeSH

Incisional hernia affects up to 20% of patients after abdominal surgery. Unlike other types of hernia, its prognosis is poor, and patients suffer from recurrence within 10 years of the operation. Currently used hernia-repair meshes do not guarantee success, but only extend the recurrence-free period by about 5 years. Most of them are nonresorbable, and these implants can lead to many complications that are in some cases life-threatening. Electrospun nanofibers of various polymers have been used as tissue scaffolds and have been explored extensively in the last decade, due to their low cost and good biocompatibility. Their architecture mimics the natural extracellular matrix. We tested a biodegradable polyester poly-ε-caprolactone in the form of nanofibers as a scaffold for fascia healing in an abdominal closure-reinforcement model for prevention of incisional hernia formation. Both in vitro tests and an experiment on a rabbit model showed promising results.

• Klíčová slova
• growth factors, hernia regeneration, in vivo, nanofibers, surgical mesh,
• MeSH
• biomechanika MeSH
• břicho chirurgie   MeSH
• buňky 3T3 MeSH
• chirurgické síťky MeSH
• hernie prevence a kontrola   MeSH
• histocytochemie MeSH
• hojení ran účinky léků   MeSH
• králíci MeSH
• mezibuněčné signální peptidy a proteiny chemie   farmakologie   terapeutické užití   MeSH
• myši MeSH
• nanovlákna chemie   terapeutické užití   MeSH
• polyestery chemie   terapeutické užití   MeSH
• polypropyleny chemie   terapeutické užití   MeSH
• pooperační komplikace prevence a kontrola   MeSH
• řízená tkáňová regenerace MeSH
• techniky uzavření břišních poranění přístrojové vybavení   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• mezibuněčné signální peptidy a proteiny MeSH
• polycaprolactone MeSH Prohlížeč
• polyestery MeSH
• polypropyleny MeSH

OBJECTIVES: We prepared 3D poly (ε-caprolactone) (PCL) nanofibre scaffolds and tested their use for seeding, proliferation, differentiation and migration of mesenchymal stem cell (MSCs). MATERIALS AND METHODS: 3D nanofibres were prepared using a special collector for common electrospinning; simultaneously, a 2D PCL nanofibre layer was prepared using a classic plain collector. Both scaffolds were seeded with MSCs and biologically tested. MSC adhesion, migration, proliferation and osteogenic differentiation were investigated. RESULTS: The 3D PCL scaffold was characterized by having better biomechanical properties, namely greater elasticity and resistance against stress and strain, thus this scaffold will be able to find broad applications in tissue engineering. Clearly, while nanofibre layers of the 2D scaffold prevented MSCs from migrating through the conformation, cells infiltrated freely through the 3D scaffold. MSC adhesion to the 3D nanofibre PCL layer was also statistically more common than to the 2D scaffold (P < 0.05), and proliferation and viability of MSCs 2 or 3 weeks post-seeding, were also greater on the 3D scaffold. In addition, the 3D PCL scaffold was also characterized by displaying enhanced MSC osteogenic differentiation. CONCLUSIONS: We draw the conclusion that all positive effects observed using the 3D PCL nanofibre scaffold are related to the larger fibre surface area available to the cells. Thus, the proposed 3D structure of the nanofibre layer will find a wide array of applications in tissue engineering and regenerative medicine.

• MeSH
• buněčná diferenciace * MeSH
• buněčné kultury přístrojové vybavení   metody   MeSH
• kultivované buňky MeSH
• lidé MeSH
• mezenchymální kmenové buňky cytologie   metabolismus   MeSH
• nanovlákna chemie   ultrastruktura   MeSH
• osteogeneze MeSH
• osteokalcin metabolismus   MeSH
• pohyb buněk MeSH
• polyestery chemie   MeSH
• povrchové vlastnosti MeSH
• proliferace buněk MeSH
• pružnost MeSH
• regenerativní lékařství MeSH
• sialoprotein vázající integrin metabolismus   MeSH
• tkáňové inženýrství MeSH
• tkáňové podpůrné struktury * MeSH
• viabilita buněk MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• osteokalcin MeSH
• polycaprolactone MeSH Prohlížeč
• polyestery MeSH
• sialoprotein vázající integrin MeSH