Berne, C*
Dotaz
Zobrazit nápovědu
BACKGROUND: Eliminating falciparum malaria in Cambodia is a top priority, requiring the implementation of novel tools and strategies to interrupt its transmission. To date, few data are available regarding the contributions to malaria transmission of symptomatic and asymptomatic carriers. METHODS: Direct-membrane and skin feeding assays (DMFAs, SFAs) were performed, using Anopheles minimus and Anopheles dirus, to determine infectivity of symptomatic falciparum-infected patients and malaria asymptomatic carriers; a subset of the latter were followed up for 2 months to assess their transmission potential. RESULTS: By microscopy and real-time polymerase chain reaction, Plasmodium falciparum gametocyte prevalence rates were, respectively, 19.3% (n = 21/109) and 44% (n = 47/109) on day (D) 0 and 17.9% (n = 5/28) and 89.3% (n = 25/28) in recrudescent patients (Drec) (RT-PCR Drec vs D0 P = .002). Falciparum malaria patient infectivity was low on D0 (6.2%; n = 3/48) and in Drec (8.3%; n = 1/12). Direct-membrane feeding assays and SFAs gave similar results. None of the falciparum (n = 0/19) and 3 of 28 Plasmodium vivax asymptomatic carriers were infectious to mosquitoes, including those that were followed up for 2 months. Overall, P. falciparum gametocytemias were low except in a few symptomatic carriers. CONCLUSIONS: Only symptomatic falciparum malaria patients were infectious to mosquito vectors at baseline and recrudescence, highlighting the need to detect promptly and treat effectively P. falciparum patients.
- MeSH
- Anopheles parazitologie MeSH
- dítě MeSH
- dospělí MeSH
- komáří přenašeči parazitologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- malárie vivax parazitologie přenos MeSH
- mladý dospělý MeSH
- paraziti patogenita MeSH
- Plasmodium falciparum patogenita MeSH
- Plasmodium vivax patogenita MeSH
- prevalence MeSH
- tropická malárie parazitologie přenos MeSH
- zvířata MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky MeSH
- práce podpořená grantem MeSH
- randomizované kontrolované studie MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Geografické názvy
- Kambodža MeSH
Insulin is known to increase blood flow in parallel to glucose uptake in skeletal muscle. However, it is not known if an increase in blood flow by itself is associated with an increase in glucose uptake in the absence of hyperinsulinemia. To investigate further this matter, the effect of increased blood flow on forearm glucose uptake was studied in the fasting state during intra-arterial infusions of two different vasodilators, metacholine and nitroprusside, in 19 hypertensive subjects. Both metacholine (4 microg/min) and nitroprusside (10 microg/min) increased resting forearm blood flow, measured by venous occlusion plethysmography, to a similar degree (180 % and 170 %, respectively, p<0.0001 for both). However, metacholine infusion increased the forearm glucose uptake from 2.0+/-0.9 (S.D.) during rest to 5.5+/-3.0 umol/min/100 ml tissue (p<0.0001), while no significant change in glucose uptake was seen during nitroprusside infusion (2.3+/-1.4 micromol/min/100 ml tissue). In conclusion, vasodilatation induced by metacholine, but not by nitroprusside, increased glucose uptake in the forearm of hypertensive patients. Thus, an increase in forearm blood flow does not necessarily improve glucose uptake in the forearm during the fasting state.
- MeSH
- dospělí MeSH
- hypertenze patofyziologie MeSH
- kinetika MeSH
- kosterní svaly metabolismus MeSH
- krevní glukóza metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- methacholinchlorid farmakologie MeSH
- nitroprusid farmakologie MeSH
- omezení příjmu potravy MeSH
- předloktí krevní zásobení MeSH
- rychlost toku krve MeSH
- senioři MeSH
- spotřeba kyslíku MeSH
- vazodilatancia farmakologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- krevní glukóza MeSH
- methacholinchlorid MeSH
- nitroprusid MeSH
- vazodilatancia MeSH
Background: Mind-body medicine (MBM) focuses on improving our understanding of how the interactions between the brain, mind, body, and behavior can be used to promote health. In this narrative review, we present the basic principles of MBM, including the introduction of a rational framework for the implementation of MBM-based interventions. We also discuss the contributions of MBM to motivation and reward systems in the brain including those that may specifically involve the mitochondria. Results: MBM can be used to promote health in patients with chronic diseases, especially conditions identified as lifestyle-related. MBM builds on salutogenesis, which is a paradigm that focuses on health (as opposed to disease) determinants and the development of individual resilience and coherence factors as a means to reduce stress, decrease the burden of disease, and improve the quality of life. This approach involves several well-known principles of self-healing and self-care. MBM interventions typically include behavioral modification techniques in conjunction with cognitive work focused on stress regulation, exercise, relaxation, meditation, and nutrition. We suggest the use of the acronym "BERN" (Behavior, Exercise, Relaxation, and Nutrition) to summarize the operational framework of this approach. Discussion: Different BERN techniques act via shared autoregulatory central nervous system (CNS) reward and motivation circuitries. These systems rely on numerous neurobiological signaling pathways with overlapping effector molecules that converge, e.g., on nitric oxide (NO) as a common effector molecule. NO is critically coupled to reward physiology, stress reduction, and self-regulation as it modulates the responses of various mitochondrial, nuclear, and chromosomal processes within brain cells. NO has also been implicated in relevant outcomes (e.g., the placebo response). Conclusions: MBM interventions typically follow the BERN model and aim to strengthen health and resilience, and reduce stress. The mechanisms of action of these processes involve the CNS reward systems and correlate with placebo and self-healing pathways.
- Klíčová slova
- behavioral medicine, integrative medicine, meditation, mitochondria, placebo, salutogenesis, self-healing, stress management,
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
Biomedicine today is experiencing a shift towards decentralized data collection, which promises enhanced reproducibility and collaboration across diverse laboratory environments. This inter-laboratory study evaluates the performance of biocytometry, a method utilizing engineered bioparticles for enumerating cells based on their surface antigen patterns. In centralized and aggregated inter-lab studies, biocytometry demonstrated significant statistical power in discriminating numbers of target cells at varying concentrations as low as 1 cell per 100,000 background cells. User skill levels varied from expert to beginner capturing a range of proficiencies. Measurement was performed in a decentralized environment without any instrument cross-calibration or advanced user training outside of a basic instruction manual. The results affirm biocytometry to be a viable solution for immunophenotyping applications demanding sensitivity as well as scalability and reproducibility and paves the way for decentralized analysis of rare cells in heterogeneous samples.
- MeSH
- analýza jednotlivých buněk * metody MeSH
- imunofenotypizace metody MeSH
- laboratoře normy MeSH
- lidé MeSH
- průtoková cytometrie metody MeSH
- reprodukovatelnost výsledků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The MS-30 (Morscher-Spotorno) cemented femoral stem is a straight, three-dimensionally tapered, collarless implant for cemented fixation in total hip replacement, manufactured from a FeCrNiMnMoNb-alloy. This paper documents the review of 4 original follow-ups performed at four orthopaedic hospitals: three at the author's institution (Basel/Switzerland 2, Pietra Ligure/Italy 1), and one at the University Orthopaedic Hospital of Heidelberg/ Germany. A total of 911 hips in 867 patients have been provided with the MS-30 stem. All stems were fixed with Palacos bone cement, the great majority with antibiotic (Gentamicine) loaded cement. The mean observation time was 10.2, 5.6, 10.2 and 6.5 years. One hundred and sixty-four hips were lost due to patients death, 121 patients were unable to come to the follow-up check. Ten patients only were lost to follow-up. The total number of hips with a clinical radiological follow-up was 597. The clinical and radiological assessment was in accordance with the International Documentation and Evaluation System (IDES) forms from the Institute of Documentation of the M.E. Müller Foundation in Berne/Switzerland. RESULTS The overall survivorship in the four follow-ups were 100% after 10 years, 98.4% and 99.2% after 5.5.years, 98.5% after 10 years and finally 96.1% after 6.5 years. The respective percentages of survival for aseptic loosening were 100%, 99.2%, 100%, 98.5% and 98.8%. CONCLUSION According to the NIH (National Institute of Health, United Kingdom) a survival rate of 95% regarding aseptic loosening justifies or even recommends further clinical use of the respective endoprosthesis system.
- MeSH
- analýza přežití MeSH
- cementování * MeSH
- lidé středního věku MeSH
- lidé MeSH
- náhrada kyčelního kloubu * MeSH
- následné studie MeSH
- protézy - design MeSH
- reoperace MeSH
- selhání protézy MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
The negative impact of the COVID-19 pandemic and distance education has significantly affected adolescents' lives. The restrictive policies negatively affected their physical and mental health and life satisfaction. It is therefore desirable to look for ways to eliminate similar negative impacts on adolescents in the future. This study aimed to identify the differences in the association between physical activity and life satisfaction in Polish adolescents before and during the pandemic. The study included 1541 participants (891 before the pandemic and 650 during the pandemic) aged 15-19 years. The International Physical Activity Questionnaire-Long Form questionnaire was used to assess physical activity, and the Bern Subjective Well-Being Questionnaire was used to evaluate life satisfaction. The objectivity of data collection and analysis before and during the pandemic was ensured by the web application the International Database for Research and Educational Support. During the pandemic, we found lower physical activity and life satisfaction in both boys and girls compared to before the pandemic. Girls with low and high physical activity levels showed significantly lower life satisfaction during the pandemic than they did before. Low life satisfaction was associated with lower physical activity, and high life satisfaction was associated with greater total physical activity. Both boys and girls with low and high life satisfaction achieved significantly fewer physical activity recommendations during the pandemic. High life satisfaction increased the likelihood of meeting the recommendations for physical activity before and during the pandemic. This study provides new insights into the differences and associations between life satisfaction and physical activity in adolescent boys and girls before and during the pandemic. It provides suggestions for the prevention of possible future restrictions in the school settings. Support for post-pandemic life satisfaction in adolescents should focus mainly on increasing vigorous physical activity and improving adolescent participation in organized physical activity settings.
- Klíčová slova
- COVID-19, Life satisfaction, Organized physical activity, Physical activity recommendations,
- MeSH
- COVID-19 * epidemiologie MeSH
- cvičení MeSH
- lidé MeSH
- mladiství MeSH
- osobní uspokojení MeSH
- pandemie MeSH
- průzkumy a dotazníky MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Physical and mental health are the basis of life satisfaction (LS), even during adolescence. The aim of this study was to identify the associations between LS and types of physical activity (PA) in Czech and Polish boys and girls. METHODS: The research involved 933 girls and 663 boys aged 15-19 years. LS was diagnosed using the Bern Subjective Well-Being Questionnaire and the WHO-5 Well-Being Index. The International Physical Activity Questionnaire-long form (IPAQ-LF) questionnaire was used to identify the types of weekly PA. RESULTS: Adolescents with the highest LS had more recreational, moderate, vigorous, and total weekly PA. The strongest associations between LS and PA were in the Czech and Polish boys and girls who participated in vigorous PA (VPA). The recommendations for VPA were fulfilled by 45% of Czech and 46% of Polish boys and 40% of Czech and 50% of Polish girls, with the highest LS. The most significant positive moderator between LS and PA was participation in organized PA. Boys with the highest LS were 1.94 times more likely to meet the weekly recommendation of VPA than boys with the lowest LS. Similarly, girls with the highest LS were 1.77 times more likely to meet these recommendations. CONCLUSIONS: Promoting both current subjective well-being and organized PA, with an emphasis on achieving the VPA recommendations, may support general LS and a healthy lifestyle in adolescents.
- Klíčová slova
- IPAQ-LF, organized physical activity, quality of life, type of physical activity, well-being,
- MeSH
- cvičení * MeSH
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- osobní uspokojení * MeSH
- průzkumy a dotazníky MeSH
- zdravý životní styl MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Polsko MeSH
Life satisfaction, an important precursor of adolescents' well-being, is linked to daily activities. Substantial changes have been noted in adolescents' daily activities over the years, raising the question of possible consequences for life satisfaction. This study aimed to explore changes in adolescents' life satisfaction and their time spent on daily activities (sleeping, engaging in sports, online gaming, school commuting, time spent at school) and further investigate the associations between adolescents' life satisfaction and these daily activities. The sample comprised 2715 adolescents from birth cohorts surveyed at four time points between 1992 and 2019. Participants were administered the Daily Activities Inventory and the Berne Questionnaire on Adolescents' Well-Being. Robust ANOVA with post hoc tests and spline regression were employed. We found cohort differences in sleep duration (8.6 h a day on average in 1992 and 7.5 in 2019). Sleep duration of 8 h and 1 h of sports activities had a beneficial effect on life satisfaction, while more than 1 h of online gaming had a negative impact. Neither school attendance nor commuting to school was associated with adolescents' life satisfaction. The results of this study can inform public-health policies to promote sleeping and sports habits in adolescence.
- Klíčová slova
- adolescents, birth cohorts, daily activities, leisure, life satisfaction, online gaming, school commuting, sleep duration, sports, time spent at school,
- MeSH
- lidé MeSH
- mladiství MeSH
- osobní uspokojení * MeSH
- průzkumy a dotazníky MeSH
- školy MeSH
- spánek * MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Despite essential progress towards understanding the evolution of cooperative behaviour, we still lack detailed knowledge about its underlying molecular mechanisms, genetic basis, evolutionary dynamics and ontogeny. An international workshop "Genetics and Development of Cooperation," organized by the University of Bern (Switzerland), aimed at discussing the current progress in this research field and suggesting avenues for future research. This review uses the major themes of the meeting as a springboard to synthesize the concepts of genetic and nongenetic inheritance of cooperation, and to review a quantitative genetic framework that allows for the inclusion of indirect genetic effects. Furthermore, we argue that including nongenetic inheritance, such as transgenerational epigenetic effects, parental effects, ecological and cultural inheritance, provides a more nuanced view of the evolution of cooperation. We summarize those genes and molecular pathways in a range of species that seem promising candidates for mechanisms underlying cooperative behaviours. Concerning the neurobiological substrate of cooperation, we suggest three cognitive skills necessary for the ability to cooperate: (i) event memory, (ii) synchrony with others and (iii) responsiveness to others. Taking a closer look at the developmental trajectories that lead to the expression of cooperative behaviours, we discuss the dichotomy between early morphological specialization in social insects and more flexible behavioural specialization in cooperatively breeding vertebrates. Finally, we provide recommendations for which biological systems and species may be particularly suitable, which specific traits and parameters should be measured, what type of approaches should be followed, and which methods should be employed in studies of cooperation to better understand how cooperation evolves and manifests in nature.
- Klíčová slova
- altruism, behaviour, indirect genetic effects, social behaviour, social effects,
- MeSH
- altruismus MeSH
- biologická evoluce * MeSH
- chování zvířat MeSH
- epigeneze genetická MeSH
- fenotyp MeSH
- genetická zdatnost MeSH
- kongresy jako téma MeSH
- kooperační chování * MeSH
- neurosekreční systémy fyziologie MeSH
- paměť MeSH
- vývojová biologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Geografické názvy
- Švýcarsko MeSH
We studied the influence of homosexual (egosynton) orientation towards the variables of subjective well-being by means of the 5 scales of the Berne Questionnaire of Subjective Well-Being on the sample of 104 men. The results prove that there is no difference between homosexually and heterosexually oriented men in the overall life satisfaction, the number of contemporary problems, the number of somatic complaints, in the rate of depressive mood and in the scale of self-esteem. This fact may be connected with the capability of coping with their homosexual orientation.
