INTRODUCTION: Replanting the inferior mesentery artery during infrarenal aortic aneurysm repair is a measure which might prevent development of colon ischemia under certain circumstances. These circumstances and patients who would benefit from this procedure are not well defined. CASE REPORT: 64-year old man underwent an elective operation on infrarenal AAA at our institution in December 2009. From preoperative CT angiography we knew about the accessory right renal artery branching directly from AAA and bilateral occlusion of hypogastric arteries. We performed open resection of AAA with implantation of a bifurcated graft. Proximal anastomosis was situated below renal arteries, distal anastomoses were bilaterally constructed on external illiac arteries. The accessory right renal artery was anastomosed into the right limb of the graft and IMA was replanted into the body of the graft. Postoperative recovery of the patient was uneventful. His follow-ups 3 and 6 months after the operation have been showing good clinical state of the patient, absence of abdominal complaints and normal levels of urea and creatinine. CT angiography which was performed 3 months after the operation discovered an occlusion of the reimplanted IMA, but patent replanted accessory right renal artery. DISCUSSION: Assessment of collateral circulation of large intestine during infrarenal AAA repair is influenced by many preoperative and intraoperative factors. Most surgeons judge the adequacy of the collateral circulation by IMA backbleeding combined with inspection of sigmoid colon after restoring aortic flow. There have been numerous attempts to replace this subjective approach with more objective methods like intraoperative colon mucosal saturation measurement, laser Doppler flowmetry, IMA stump pressures, photophletyzmographic technique. Even though these methods describe conditions when a collateral circulation of rectosigmoid is inadequate after IMA ligature, they are unable to fully eliminate the occurrence of colon ischemia because of its multifactorial nature. Solving the problem of collateral circulation of the large intestine represents only a part of the obstacle presented by colon ischemia after infrarenal AAA repair. CONCLUSION: IMA replantation during infrarenal AAA repair does not fully prevent an occurance of colon ischemia. On the other side, this moneuver does not increase perioperative morbidity, nor prolongs an operation significantly. Our policy is to replant IMA whenever we thing the circulation of large intestine is under threat or in borderline situations.

• MeSH
• aneurysma břišní aorty diagnostické zobrazování   chirurgie   MeSH
• angiografie MeSH
• arteria mesenterica inferior chirurgie   MeSH
• arteria renalis chirurgie   MeSH
• cévy - implantace protéz * MeSH
• ischemie prevence a kontrola   MeSH
• kolon krevní zásobení   MeSH
• lidé středního věku MeSH
• lidé MeSH
• počítačová rentgenová tomografie MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

General transcription factor TFIID is a key component of RNA polymerase II transcription initiation. Human TFIID is a megadalton-sized complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core promoter DNA, recognizing the TATA-box. We identified a ternary complex formed by TBP and the histone fold (HF) domain-containing TFIID subunits TAF11 and TAF13. We demonstrate that TAF11/TAF13 competes for TBP binding with TATA-box DNA, and also with the N-terminal domain of TAF1 previously implicated in TATA-box mimicry. In an integrative approach combining crystal coordinates, biochemical analyses and data from cross-linking mass-spectrometry (CLMS), we determine the architecture of the TAF11/TAF13/TBP complex, revealing TAF11/TAF13 interaction with the DNA binding surface of TBP. We identify a highly conserved C-terminal TBP-interaction domain (CTID) in TAF13, which is essential for supporting cell growth. Our results thus have implications for cellular TFIID assembly and suggest a novel regulatory state for TFIID function.

• Klíčová slova
• S. cerevisiae, TBP associated factors, TFIID, biochemistry, biophysics, core promoter DNA, gene regulation, histone fold domain, human, structural biology, transcription factors,
• MeSH
• DNA metabolismus   MeSH
• faktory asociované s proteinem vázajícím TATA box chemie   metabolismus   MeSH
• histonacetyltransferasy metabolismus   MeSH
• hmotnostní spektrometrie MeSH
• konformace proteinů MeSH
• krystalografie rentgenová MeSH
• lidé MeSH
• mapování interakce mezi proteiny MeSH
• promotorové oblasti (genetika) MeSH
• protein vázající TATA box chemie   metabolismus   MeSH
• transkripční faktor TFIID chemie   metabolismus   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA MeSH
• faktory asociované s proteinem vázajícím TATA box MeSH
• histonacetyltransferasy MeSH
• protein vázající TATA box MeSH
• TAF11 protein, human MeSH Prohlížeč
• TAF13 protein, human MeSH Prohlížeč
• TATA-binding protein associated factor 250 kDa MeSH Prohlížeč
• TBP protein, human MeSH Prohlížeč
• transkripční faktor TFIID MeSH

A study of intestinal helminthic infections of 52 cats (Felis catus) was undertaken in Calabar, Nigeria. Direct smear and zinc sulphate floatation technique were utilized. The following helminths were observed: Toxocara cati (28.85%), Ancylostoma tubaeforme (19.23%), Trichuris felis (5.77%), Dipylidium caninum (23.08%) and Taenia taeniaeformis (9.61%). The worm burdens were generally low, ranging from 2 to 20 per cat. No definite pattern of infection was observed with regard to sex. Trematodes were not seen. The zoonotic and public health aspects of some of these helminths are discussed.

• MeSH
• helmintóza epidemiologie   MeSH
• helmintózy zvířat * MeSH
• kočky MeSH
• nemoci koček epidemiologie   MeSH
• parazitární nemoci střev epidemiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Nigérie MeSH

During the dissection of an 82-year-old male specimen, we found an atypical variant of the aortic arch with 5 branches instead of the usual 3. The additional arteries were identified as the IMA thyroid artery (the artery of Neubauer) and the left vertebral artery, which arose directly from the aortic arch. The IMA artery was located between the origin of the brachiocephalic trunk and the left common carotid artery. The left vertebral artery was situated between the origin of the left common carotid artery and the left subclavian artery. No other variations in the origins of major vessels in the thoracic region, head, and neck were observed. The frequency of the left vertebral artery arising directly from the aortic arch is estimated to be 3-8%, and when combined with the IMA artery arising directly from the aortic arch, which occurs in less than 1% of cases, the variant described appears to be extremely rare. In this paper, we present the exact measurements of the arteries we found, their topography relative to other significant anatomical structures, and the potential clinical significance of these variations. The work is accompanied by diagrams and photographs.

• Klíčová slova
• IMA thyroid artery, aortic arch, left vertebral artery,
• Publikační typ
• časopisecké články MeSH

This article highlights an unusual and unilateral variation in the blood supply to the inferior portion of the thyroid gland observed on the right lobe during anatomy dissection course. The rare variation of the occurrence of two anomalous arteries: the middle thyroid artery and the aberrant accessory inferior thyroid artery, and one uncommon variant, the thyroid ima artery, was detected in an adult female cadaver. The two generally constant arteries, the superior thyroid artery and the inferior thyroid artery, have been found in their usual anatomical location. Both the middle thyroid artery and aberrant accessory inferior thyroid artery arose from the right common carotid artery. The middle thyroid artery coursed as a very short branch ventromedially to enter the inferior lateral portion of the right lobe of the thyroid gland. It was at the same level, in which the inferior thyroid artery reached the lateral border of the thyroid gland. The aberrant accessory inferior thyroid artery originated similarly, from the ventromedial surface of the right common carotid artery and passed to supply the inferior pole of the right lobe. The thyroid ima artery was found to arise from the brachiocephalic trunk, entering the isthmus of the thyroid gland. Information about the embryological background might be helpful to clarify why such a type of variation occurs. It is necessary to understand the possible existence of this anomaly, to carry out successful radical neck dissection and to minimize the risk of postoperative complications in patients.

• Klíčová slova
• Aberrant accessory inferior thyroid artery, Middle thyroid artery, Terminology, Thyroid ima artery, Variations,
• MeSH
• anatomická variace MeSH
• arteria carotis communis anatomie a histologie   MeSH
• arteria subclavia anatomie a histologie   MeSH
• disekce MeSH
• lidé MeSH
• mrtvola MeSH
• senioři MeSH
• štítná žláza krevní zásobení   MeSH
• truncus brachiocephalicus anatomie a histologie   MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

• Publikační typ
• časopisecké články MeSH

We aimed to perform a chemical analysis of both Alibernet red wine and an alcohol-free Alibernet red wine extract (AWE) and to investigate the effects of AWE on nitric oxide and reactive oxygen species production as well as blood pressure development in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHRs). Total antioxidant capacity together with total phenolic and selected mineral content was measured in wine and AWE. Young 6-week-old male WKY and SHR were treated with AWE (24,2 mg/kg/day) for 3 weeks. Total NOS and SOD activities, eNOS and SOD1 protein expressions, and superoxide production were determined in the tissues. Both antioxidant capacity and phenolic content were significantly higher in AWE compared to wine. The AWE increased NOS activity in the left ventricle, aorta, and kidney of SHR, while it did not change NOS activity in WKY rats. Similarly, increased SOD activity in the plasma and left ventricle was observed in SHR only. There were no changes in eNOS and SOD1 expressions. In conclusion, phenolics and minerals included in AWE may contribute directly to increased NOS and SOD activities of SHR. Nevertheless, 3 weeks of AWE treatment failed to affect blood pressure of SHR.

• MeSH
• antioxidancia chemie   farmakologie   MeSH
• aorta účinky léků   enzymologie   MeSH
• hypertenze metabolismus   patologie   MeSH
• krysa rodu Rattus MeSH
• ledviny účinky léků   enzymologie   MeSH
• minerály analýza   farmakologie   MeSH
• oxid dusnatý metabolismus   MeSH
• polyfenoly analýza   farmakologie   MeSH
• potkani inbrední SHR MeSH
• potkani inbrední WKY MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• rostlinné extrakty chemie   farmakologie   MeSH
• srdeční komory účinky léků   enzymologie   MeSH
• superoxiddismutasa 1 MeSH
• superoxiddismutasa metabolismus   MeSH
• synthasa oxidu dusnatého, typ III metabolismus   MeSH
• víno analýza   MeSH
• Vitis chemie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• minerály MeSH
• oxid dusnatý MeSH
• polyfenoly MeSH
• reaktivní formy kyslíku MeSH
• rostlinné extrakty MeSH
• Sod1 protein, rat MeSH Prohlížeč
• superoxiddismutasa 1 MeSH
• superoxiddismutasa MeSH
• synthasa oxidu dusnatého, typ III MeSH

The authors compare three groups of patients operated at the cardiosurgical clinic of the Faculty Hospital in Hradec Králové on account of ischaemic heart disease. Group A comprised 50 patients where for revascularization of the myocardium venous grafts were used, group B comprised 50 patients where also the mammary artery was used/IMA/. The postoperative blood losses are compared and the number of administered blood transfusions in the two groups. Group C is formed by 50 patients where after preparation of the IMA aprotinin/100 000 u./was administered locally. The authors provide evidence that the use of IMA increases significantly the postoperative blood losses/in group A 675 ml +/- 352.9, in group B 1232 ml +/- 336.5/and increases the number of required transfusions/group A 2.44 +/- 1, group B 3.45 +/- 1/. By local aprotinin application to the wound surface during preparation of IMA the authors reduced in group C the blood losses to 896 +/- 231.9 ml and the number of administered transfusions to 2.74 +/- 0.8.

• MeSH
• aplikace lokální MeSH
• aprotinin aplikace a dávkování   MeSH
• krvácení při operaci * MeSH
• lidé středního věku MeSH
• lidé MeSH
• mamární tepny MeSH
• revaskularizace myokardu * MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• aprotinin MeSH

BACKGROUND: The brain stem contains important nuclei that control cardiovascular function via the sympathetic nervous system (SNS), which is strongly influenced by nitric oxide. Its biological activity is also largely determined by oxygen free radicals. Despite many experimental studies, the role of AT1R-NAD(P)H oxidase-superoxide pathway in NO-deficiency is not yet sufficiently clarified. We determined changes in free radical signaling and antioxidant and detoxification response in the brain stem of young and adult Wistar rats during chronic administration of exogenous NO inhibitors. METHODS: Young (4 weeks) and adult (10 weeks) Wistar rats were treated with 7-nitroindazole (7-NI group, 10 mg/kg/day), a specific nNOS inhibitor, with NG-nitro-L-arginine-methyl ester (L-NAME group, 50 mg/kg/day), a nonspecific NOS inhibitor, and with drinking water (Control group) during 6 weeks. Systolic blood pressure was measured by non-invasive plethysmography. Expression of genes (AT1R, AT2R, p22phox, SOD and NOS isoforms, HO-1, MDR1a, housekeeper GAPDH) was identified by real-time PCR. NOS activity was detected by conversion of [3H]-L-arginine to [3H]-L-citrulline and SOD activity was measured using UV VIS spectroscopy. RESULTS: We observed a blood pressure elevation and decrease in NOS activity only after L-NAME application in both age groups. Gene expression of nNOS (youngs) and eNOS (adults) in the brain stem decreased after both inhibitors. The radical signaling pathway triggered by AT1R and p22phox was elevated in L-NAME adults, but not in young rats. Moreover, L-NAME-induced NOS inhibition increased antioxidant response, as indicated by the observed elevation of mRNA SOD3, HO-1, AT2R and MDR1a in adult rats. 7-NI did not have a significant effect on AT1R-NADPH oxidase-superoxide pathway, yet it affected antioxidant response of mRNA expression of SOD1 and stimulated total activity of SOD in young rats and mRNA expression of AT2R in adult rats. CONCLUSION: Our results show that chronic NOS inhibition by two different NOS inhibitors has age-dependent effect on radical signaling and antioxidant/detoxificant response in Wistar rats. While 7-NI had neuroprotective effect in the brain stem of young Wistar rats, L-NAME- induced NOS inhibition evoked activation of AT1R-NAD(P)H oxidase pathway in adult Wistar rats. Triggering of the radical pathway was followed by activation of protective compensation mechanism at the gene expression level.

• Klíčová slova
• Antioxidant response, Brain stem, NOS inhibition, Radical signaling,
• MeSH
• antioxidancia metabolismus   MeSH
• indazoly farmakologie   MeSH
• inhibitory enzymů farmakologie   MeSH
• krysa rodu Rattus MeSH
• metabolická inaktivace * MeSH
• mozkový kmen účinky léků   metabolismus   MeSH
• NG-nitroargininmethylester farmakologie   MeSH
• oxid dusnatý nedostatek   MeSH
• potkani Wistar MeSH
• synthasa oxidu dusnatého antagonisté a inhibitory   MeSH
• věkové faktory MeSH
• volné radikály metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 7-nitroindazole MeSH Prohlížeč
• antioxidancia MeSH
• indazoly MeSH
• inhibitory enzymů MeSH
• NG-nitroargininmethylester MeSH
• oxid dusnatý MeSH
• synthasa oxidu dusnatého MeSH
• volné radikály MeSH

Activation of nuclear factor-κB (NF-κB) by increased production of reactive oxygen species (ROS) might induce transcription and expression of different antioxidant enzymes and also of nitric oxide synthase (NOS) isoforms. Thus, we aimed at studying the effect of NF-κB inhibition, caused by JSH-23 (4-methyl-N (1)-(3-phenyl-propyl)-benzene-1,2-diamine) injection, on ROS and NO generation in hereditary hypertriglyceridemic (HTG) rats. 12-week-old, male Wistar and HTG rats were treated with JSH-23 (bolus, 10 μmol, i.v.). After one week, blood pressure (BP), superoxide dismutase (SOD) activity, SOD1, endothelial NOS (eNOS), and NF-κB (p65) protein expressions were higher in the heart of HTG rats compared to control rats. On the other hand, NOS activity was decreased. In HTG rats, JSH-23 treatment increased BP and heart conjugated dienes (CD) concentration (measured as the marker of tissue oxidative damage). Concomitantly, SOD activity together with SOD1 expression was decreased, while NOS activity and eNOS protein expression were increased significantly. In conclusion, NF-κB inhibition in HTG rats led to decreased ROS degradation by SOD followed by increased oxidative damage in the heart and BP elevation. In these conditions, increased NO generation may represent rather a counterregulatory mechanism activated by ROS. Nevertheless, this mechanism was not sufficient enough to compensate BP increase in HTG rats.

• MeSH
• exprese genu účinky léků   MeSH
• fenylendiaminy farmakologie   MeSH
• glutathion analýza   MeSH
• hyperlipoproteinemie typ IV patologie   veterinární   MeSH
• krevní tlak účinky léků   MeSH
• krysa rodu Rattus MeSH
• myokard metabolismus   MeSH
• oxid dusnatý metabolismus   MeSH
• oxidační stres účinky léků   MeSH
• potkani Wistar MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• srdeční komory metabolismus   MeSH
• superoxiddismutasa genetika   metabolismus   MeSH
• synthasa oxidu dusnatého, typ III genetika   metabolismus   MeSH
• synthasa oxidu dusnatého genetika   metabolismus   MeSH
• tělesná hmotnost účinky léků   MeSH
• transkripční faktor RelA genetika   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• 4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine MeSH Prohlížeč
• fenylendiaminy MeSH
• glutathion MeSH
• oxid dusnatý MeSH
• reaktivní formy kyslíku MeSH
• superoxiddismutasa MeSH
• synthasa oxidu dusnatého, typ III MeSH
• synthasa oxidu dusnatého MeSH
• transkripční faktor RelA MeSH