• MeSH
• glutathion-S-transferasa fí genetika   MeSH
• glutathiontransferasa genetika   MeSH
• látky znečišťující životní prostředí krev   MeSH
• lidé MeSH
• polychlorované bifenyly krev   MeSH
• polymorfismus genetický * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• komentáře MeSH
• úvodníky MeSH
• Názvy látek
• glutathion-S-transferasa fí MeSH
• glutathione S-transferase T1 MeSH Prohlížeč
• glutathiontransferasa MeSH
• látky znečišťující životní prostředí MeSH
• polychlorované bifenyly MeSH

Phthalates are endocrine disruptors frequently occurring in the general and industrial environment and in many industrial products. Moreover, they are also suspected of being carcinogenic, teratogenic, and mutagenic, and they show diverse toxicity profiles depending on their structures. The European Union and the United States Environmental Protection Agency (US EPA) have included many phthalates in the list of priority substances with potential endocrine-disrupting action. They are: dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), butylbenzyl phthalate (BBP), diethylhexyl phthalate (DEHP), di-iso-nonyl phthalate (DINP), di-iso-decyl phthalate (DIDP), di-n-decyl phthalate (DnDP), and dioctyl phthalate (DOP). There is an ever-increasing demand for new analytical methods suitable for monitoring different phthalates in various environmental, biological, and other matrices. Separation and spectrometric methods are most frequently used. However, modern electroanalytical methods can also play a useful role in this field because of their high sensitivity, reasonable selectivity, easy automation, and miniaturization, and especially low investment and running costs, which makes them suitable for large-scale monitoring. Therefore, this review outlines possibilities and limitations of various analytical methods for determination of endocrine-disruptor phthalate esters in various matrices, including somewhat neglected electroanalytical methods.

• Klíčová slova
• Carcinogens, endocrine disruptors, phthalates,
• MeSH
• elektrochemické techniky * MeSH
• endokrinní disruptory analýza   MeSH
• estery analýza   MeSH
• kyseliny ftalové analýza   MeSH
• molekulární struktura MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• endokrinní disruptory MeSH
• estery MeSH
• kyseliny ftalové MeSH
• phthalic acid MeSH Prohlížeč

This review provides a brief survey of the biological effects of selected endocrine-disrupting compounds that are formed after internal exposure of organisms. Further, the present analytical methods available for the determination of these compounds in foodstuffs are critically evaluated. The attention is primarily devoted to the methods for sample pretreatment, which are the main source of errors and are usually the most time-consuming step of the whole analysis. This review is focused on selected natural and synthetic estrogens, estrogen conjugates, and chemical additives used in the plastic industry that can act as estrogen mimics.

• Klíčová slova
• Endocrine-disrupting compounds, Food analysis, Instrumental methods,
• MeSH
• analýza potravin * MeSH
• endokrinní disruptory analýza   MeSH
• estrogeny analýza   MeSH
• lidé MeSH
• plastické hmoty chemie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• endokrinní disruptory MeSH
• estrogeny MeSH
• plastické hmoty MeSH

Obesogens, as environmental endocrine-disrupting chemicals, are supposed to have had an impact on the prevalence of rising obesity around the world over the last forty years. These chemicals are probably able to contribute not only to the development of obesity and metabolic disturbances in individuals, but also in their progeny, having the capability to epigenetically reprogram genetically inherited set-up points for body weight and body composition control during critical periods of development, such as fetal, early life, and puberty. In individuals, they may act on myriads of neuro-endocrine-immune metabolic regulatory pathways, leading to pathophysiological consequences in adipogenesis, lipogenesis, lipolysis, immunity, the influencing of central appetite and energy expenditure regulations, changes in gut microbiota-intestine functioning, and many other processes. Evidence-based medical data have recently brought much more convincing data about associations of particular chemicals and the probability of the raised risk of developing obesity. Foods are the main source of obesogens. Some obesogens occur naturally in food, but most are environmental chemicals, entering food as a foreign substance, whether in the form of contaminants or additives, and they are used in a large amount in highly processed food. This review article contributes to a better overview of obesogens, their occurrence in foods, and their impact on the human organism.

• Klíčová slova
• adipose tissue, food, metabolic disruptors, metabolic syndrome, obesity, obesogens, systematic low-grade inflammation,
• MeSH
• adipogeneze MeSH
• endokrinní disruptory * toxicita   MeSH
• lidé MeSH
• obezita epidemiologie   etiologie   MeSH
• potraviny MeSH
• vystavení vlivu životního prostředí * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• endokrinní disruptory * MeSH

The prevalence of metabolic diseases, such as obesity, diabetes, metabolic syndrome and chronic liver diseases among others, has been rising for several years. Epidemiology and mechanistic (in vivo, in vitro and in silico) toxicology have recently provided compelling evidence implicating the chemical environment in the pathogenesis of these diseases. In this review, we will describe the biological processes that contribute to the development of metabolic diseases targeted by metabolic disruptors, and will propose an integrated pathophysiological vision of their effects on several organs. With regard to these pathomechanisms, we will discuss the needs, and the stakes of evolving the testing and assessment of endocrine disruptors to improve the prevention and management of metabolic diseases that have become a global epidemic since the end of last century.

• Klíčová slova
• TBT, appetite, bisphenol, dioxin, inflammation, insulin resistance, microbiota, perfluorinated compounds, phthalate,
• MeSH
• endokrinní disruptory * toxicita   MeSH
• fenoly MeSH
• lidé MeSH
• metabolický syndrom * MeSH
• obezita chemicky indukované   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• endokrinní disruptory * MeSH
• fenoly MeSH

Endocrine disrupting chemicals (EDCs) are exogenous substances that interfere with the endocrine system and cause adverse effects. We aimed to classify the effects of 24 known EDCs, prevalent in certain occupations, according to four modes of action (estrogenic, antiestrogenic, androgenic, and/or antiandrogenic). A literature search, stratified into four types of literature was conducted (namely: national and international agency reports; review articles; primary studies; ToxCastTM). The state of the evidence of each EDC on sex hormone function was summarized and reviewed by an expert panel. For each mode of action, the experts evaluated the likelihood of endocrine disruption in five categories: "No", "Unlikely", "Possibly", "Probably", and "Yes". Seven agents were categorized as "Yes," or having strong evidence for their effects on sex hormone function (antiandrogenic: lead, arsenic, butylbenzyl phthalate, dibutyl phthalate, dicyclohexyl phthalate; estrogenic: nonylphenol, bisphenol A). Nine agents were categorized as "Probable," or having probable evidence (antiandrogenic: bis(2-ethylhexyl)phthalate, nonylphenol, toluene, bisphenol A, diisononyl phthalate; androgenic: cadmium; estrogenic: copper, cadmium and; anti-estrogenic: lead). Two agents (arsenic, polychlorinated biphenyls) had opposing conclusions supporting both "probably" estrogenic and antiestrogenic effects. This synthesis will allow researchers to evaluate the health effects of selected EDCs with an added level of precision related to the mode of action.

• Klíčová slova
• Androgenic, Antiandrogenic, Antiestrogenic, Endocrine disruptor, Estrogenic, Occupational exposures,
• MeSH
• dibutylftalát MeSH
• endokrinní disruptory * MeSH
• lidé MeSH
• mínění MeSH
• pohlavní steroidní hormony MeSH
• pracovní expozice * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• dibutylftalát MeSH
• endokrinní disruptory * MeSH
• pohlavní steroidní hormony MeSH

Surface and treated wastewater are contaminated with highly complex mixtures of micropollutants, which may cause numerous adverse effects, often mediated by endocrine disruption. However, there is limited knowledge regarding some important modes of action, such as interference with thyroid hormone (TH) regulation, and the compounds driving these effects. This study describes an effective approach for the identification of compounds with the potential to bind to transthyretin (TTR; protein distributing TH to target tissues), based on their specific separation in a pull-down assay followed by non-target analysis (NTA). The method was optimized with known TTR ligands and applied to complex water samples. The specific separation of TTR ligands provided a substantial reduction of chromatographic features from the original samples. The applied NTA workflow resulted in the identification of 34 structures. Twelve compounds with available standards were quantified in the original extracts and their TH-displacement potency was confirmed. Eleven compounds were discovered as TTR binders for the first time and linear alkylbenzene sulfonates (LAS) were highlighted as contaminants of concern. Pull-down assay combined with NTA proved to be a well-functioning approach for the identification of unknown bioactive compounds in complex mixtures with great application potential across various biological targets and environmental compartments.

• Klíčová slova
• Affinity purification, Mixtures, Protein expression, Thyroid hormone signaling, Wastewater,
• MeSH
• chemické látky znečišťující vodu * analýza   chemie   MeSH
• endokrinní disruptory * chemie   analýza   MeSH
• hmotnostní spektrometrie metody   MeSH
• ligandy MeSH
• odpadní voda chemie   MeSH
• prealbumin * chemie   metabolismus   analýza   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chemické látky znečišťující vodu * MeSH
• endokrinní disruptory * MeSH
• ligandy MeSH
• odpadní voda MeSH
• prealbumin * MeSH

Mixture toxicity, including agonistic and antagonistic effects, is an unrevealed environmental problem. Estrogenic endocrine disruptors are known to cause adverse effects for aquatic biota, but causative chemicals and their contributions to the total activity in sewage sludge remain unknown. Therefore, advanced analytical methods, a yeast bioassay and mixture toxicity models were concurrently applied for the characterization of 8 selected sludges with delectable estrogenic activity (and 3 sludges with no activity as blanks) out of 25 samples from wastewater treatment plants (WWTPs). The first applied full logistic model adequately explained total activity by considering the concentrations of the monitored compounds. The results showed that the activity was primarily caused by natural estrogens in municipal WWTP sludge. Nevertheless, activity in a sample originating from a car-wash facility was dominantly caused by partial agonists - nonylphenols - and only a model enabling prediction of all dose-response curve parameters of the final mixture curve explained these results. Antiestrogenic effects were negligible, and effect-directed analysis identified the causative chemicals.

• Klíčová slova
• Agonistic activity, Antagonistic activity, Constructed wetland, Mixture toxicity, WWTP sludge,
• MeSH
• antagonisté estrogenu MeSH
• chemické látky znečišťující vodu * analýza   toxicita   MeSH
• endokrinní disruptory * analýza   toxicita   MeSH
• estrogeny toxicita   MeSH
• odpadní vody MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antagonisté estrogenu MeSH
• chemické látky znečišťující vodu * MeSH
• endokrinní disruptory * MeSH
• estrogeny MeSH
• odpadní vody MeSH

Bisphenol A (BPA), a synthetic chemical widely used in the production of polycarbonate plastic and epoxy resins, has been associated with a variety of adverse effects in humans including metabolic, immunological, reproductive, and neurodevelopmental effects, raising concern about its health impact. In the EU, it has been classified as toxic to reproduction and as an endocrine disruptor and was thus included in the candidate list of substances of very high concern (SVHC). On this basis, its use has been banned or restricted in some products. As a consequence, industries turned to bisphenol alternatives, such as bisphenol S (BPS) and bisphenol F (BPF), which are now found in various consumer products, as well as in human matrices at a global scale. However, due to their toxicity, these two bisphenols are in the process of being regulated. Other BPA alternatives, whose potential toxicity remains largely unknown due to a knowledge gap, have also started to be used in manufacturing processes. The gradual restriction of the use of BPA underscores the importance of understanding the potential risks associated with its alternatives to avoid regrettable substitutions. This review aims to summarize the current knowledge on the potential hazards related to BPA alternatives prioritized by European Regulatory Agencies based on their regulatory relevance and selected to be studied under the European Partnership for the Assessment of Risks from Chemicals (PARC): BPE, BPAP, BPP, BPZ, BPS-MAE, and TCBPA. The focus is on data related to toxicokinetic, endocrine disruption, immunotoxicity, developmental neurotoxicity, and genotoxicity/carcinogenicity, which were considered the most relevant endpoints to assess the hazard related to those substances. The goal here is to identify the data gaps in BPA alternatives toxicology and hence formulate the future directions that will be taken in the frame of the PARC project, which seeks also to enhance chemical risk assessment methodologies using new approach methodologies (NAMs).

• Klíčová slova
• Bisphenol A alternatives, carcinogenesis, developmental neurotoxicity, endocrine disruption, genotoxicity, human health, immunotoxicity, metabolism, toxicokinetic,
• MeSH
• benzhydrylové sloučeniny * chemie   toxicita   MeSH
• endokrinní disruptory * chemie   toxicita   MeSH
• hodnocení rizik metody   MeSH
• lidé MeSH
• sulfony toxicita   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• benzhydrylové sloučeniny * MeSH
• bisphenol A MeSH Prohlížeč
• bisphenol F MeSH Prohlížeč
• bisphenol S MeSH Prohlížeč
• endokrinní disruptory * MeSH
• sulfony MeSH

The white rot fungus Pleurotus ostreatus HK 35, which is also an edible industrial mushroom commonly cultivated in farms, was tested in the degradation of typical representatives of endocrine disrupters (EDCs; bisphenol A, estrone, 17β-estradiol, estriol, 17α-ethinylestradiol, triclosan and 4-n-nonylphenol); its degradation efficiency under model laboratory conditions was greater than 90% within 12 days and better than that of another published strain P. ostreatus 3004. A spent mushroom substrate from a local farm was tested for its applicability in various batch and trickle-bed reactors in degrading EDCs in model fortified and real communal wastewater. The reactors were tested under various regimes including a pilot-scale trickle-bed reactor, which was finally tested at a wastewater treatment plant. The result revealed that the spent substrate is an efficient biodegradation agent, where the fungus was usually able to remove about 95% of EDCs together with suppression of the estrogenic activity of the sample. The results showed the fungus was able to operate in the presence of bacterial microflora in wastewater without any substantial negative effects on the degradation abilities. Finally, a pilot-scale trickle-bed reactor was installed in a wastewater treatment plant and successfully operated for 10days, where the bioreactor was able to remove more than 76% of EDCs present in the wastewater.

• Klíčová slova
• Bioreactor, Endocrine disruptors, Ligninolytic fungi, Pleurotus ostreatus, Wastewater treatment,
• MeSH
• biodegradace MeSH
• bioreaktory mikrobiologie   MeSH
• chemické látky znečišťující vodu analýza   metabolismus   MeSH
• endokrinní disruptory analýza   izolace a purifikace   metabolismus   MeSH
• odpadní voda chemie   MeSH
• Pleurotus metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• endokrinní disruptory MeSH
• odpadní voda MeSH