The glutathione S-transferases (GSTs) are a family of multifunctional enzymes involved in cellular detoxification. The aim of this study was to evaluate the effect of albendazole--drug of choice for trichinellosis--on the total activity and kinetics of cytosolic GST in the mouse intestines during experimental trichinellosis. Our results showed a statistically significant decrease in the total GST activity both in the small and large intestines of the mice infected with the nematode Trichinella spiralis (Owen, 1835) and treated with albendazole, compared with the control mice that were infected but untreated with the drug. Furthermore, albendazole administration modified the kinetics of substrate saturation of GST in the intestines of the infected mice because the drug caused changes in Michaelis constant values of this enzyme. Based on our observations, we suggest that the quaternary structure of GST from the mouse intestines is impacted by this drug during trichinellosis.

• MeSH
• albendazol farmakologie   MeSH
• anthelmintika farmakologie   MeSH
• glutathiontransferasa genetika   metabolismus   MeSH
• myši MeSH
• střeva účinky léků   enzymologie   MeSH
• trichinelóza farmakoterapie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• albendazol MeSH
• anthelmintika MeSH
• glutathiontransferasa MeSH

• Klíčová slova
• INTESTINES *,
• MeSH
• lidé MeSH
• střelné rány * MeSH
• střeva * MeSH
• tenké střevo * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

• Klíčová slova
• INTESTINES *, ROENTGENOGRAPHY *,
• MeSH
• rentgendiagnostika * MeSH
• střeva * MeSH
• tenké střevo * MeSH
• Publikační typ
• časopisecké články MeSH

The aim of the study was to explore production of seven biogenic amines (phenylethylamine, histamine, cadaverine, tyramine, putrescine, spermine and/or spermidine) by selected staphylococci and enterococci. Thirty three enterococcal strains isolated from rabbit meat (Oryctolagus cuniculus f. domesticus) and 21 staphylococcal strains isolated from intestinal content of trout (Salmo trutta morpha fario) were tested. Production of biogenic amines was evaluated after cultivation of the tested microorganisms in the de Man, Rogosa and Sharpe Broth (enterococci) or in the Brain Heart Infusion Broth (staphylococci). Both the above cultivation media were enriched with selected amino acids (histidine, tyrosine, arginine, ornithine and lysine; 2g/L each) serving as precursors of biogenic amines. After cultivation, levels of the monitored biogenic amines in broths were analysed by a high performance liquid chromatography equipped with a UV/VIS DAD detector. Among 21 staphylococci, 18 strains produced tyramine or cadaverine, 13 strains formed putrescine or phenylethylamine and only one strain generated histamine. Two staphylococcal strains produced cadaverine levels above 1000 mg/L. Among 33 enterococcal strains, 27 formed cadaverine, 18 strains produced tyramine, 10 strains generated phenylethylamine, and 2 strains gave putrescine. Most of the tyramine producing enterococci generated more than 1000 mg/L of this biogenic amine. Production of spermine or spermidine by the studied strains was not proved.

• MeSH
• biogenní aminy analýza   biosyntéza   MeSH
• dekarboxylace MeSH
• Enterococcus klasifikace   růst a vývoj   izolace a purifikace   metabolismus   MeSH
• králíci * MeSH
• maso mikrobiologie   MeSH
• pstruh * MeSH
• Staphylococcus klasifikace   růst a vývoj   izolace a purifikace   metabolismus   MeSH
• střeva mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• králíci * MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biogenní aminy MeSH

Extensive intestinal resections in inborn intestinal atresias are the second most frequent cause of the short gut syndrome. Because treatment of this condition is so far minimal, prevention is of fundamental importance. One possible approach is tapering of the gut, i.e. longitudinal antimesenterial resection of the gut. At the Clinic of Paediatric Surgery in 1991-1995 30 patients with inborn atresias of the gut were operated (17 atresias of the duodenum, 11 atresias of the small intestine, 2 atresias of the large intestine). Six patients (20%) died. In 2 patients (one girl with atresia of the colon and one boy with atresia of the jejunum) developed dilatation of the gut orally from the site of resection of the atresia and a chronic subileous condition. Instead of resection of the dilated portion the gut was modelled by tapering. In both children the passage improved and the children thrive. Based on data in the literature and their own experience the authors assume that tapering of the gut should supplement primarily high jejunal atresia, apple peel syndrome and extensive dilatation of the jejunum. Tapering cannot be used above the aganglionic portion of the intestine.

• MeSH
• atrézie střev chirurgie   MeSH
• chirurgie operační metody   MeSH
• lidé MeSH
• novorozenec MeSH
• pooperační komplikace prevence a kontrola   MeSH
• střeva chirurgie   MeSH
• syndrom krátkého střeva etiologie   prevence a kontrola   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• novorozenec MeSH
• ženské pohlaví MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• kazuistiky MeSH

A group of 637 golden hamsters [Mesocricetus auratus] was studied and 64 of them found suffering from wet tail syndrome. They had a regional epithelial proliferation of the gut often reminding of a tumour associated with unspecified bacilliform organisms in enterocytes. Three adult females showed a similar lesion lacking of microbes with intestinal adenocarcinoma instead. Its diffuse growth reaching mesentery was combined with secondaries in mesenteric lymph nodes. Nine older hamsters showed a malignant intestinal lymphoma in similar conditions. Mutual relations of the findings were discussed. Wet tail syndrome seemed to be an intestinal precancer.

• MeSH
• adenokarcinom ultrastruktura   veterinární   MeSH
• křečci praví * MeSH
• křeček rodu Mesocricetus * MeSH
• lymfom veterinární   MeSH
• nemoci hlodavců patologie   MeSH
• střeva ultrastruktura   MeSH
• střevní nádory ultrastruktura   veterinární   MeSH
• zvířata MeSH
• Check Tag
• křečci praví * MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH

• Klíčová slova
• INTESTINES/radiography *,
• MeSH
• rentgendiagnostika MeSH
• střeva diagnostické zobrazování   MeSH
• Publikační typ
• časopisecké články MeSH

AIMS: Enterococci associated with garden snails (Helix aspersa) were studied in order to obtain reliable species identification and characterization. METHODS AND RESULTS: Twelve yellow-pigmented and motile enterococci, isolated from the intestines of garden snails, were phenotypically close to Enterococcus casseliflavus, but they showed certain unusual biochemical characteristics. tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all strains studied into two groups, in full agreement with biochemical test results. 16S rDNA sequencing, DNA base composition analysis and DNA-DNA hybridization results showed unambiguously that the enterococci studied belonged to the species Ent. casseliflavus. The representative strains of described ecovars were deposited in the Czech Collection of Microorganisms (CCM) as Ent. casseliflavus CCM 4868, 4869, 4870 and 4871. CONCLUSIONS: Enterococcus casseliflavus associated with garden snails can be subdivided into groups. SIGNIFICANCE AND IMPACT OF THE STUDY: Enterococcus casseliflavus differs from other enterococcal species in that it is typically associated with plants, soil, water and invertebrate animals. The different groups that can be found in these widely occurring bacteria are possibly source-specific ecovars, as exemplified by the Ent. casseliflavus inhabiting the intestines of snails.

• MeSH
• biologické pigmenty metabolismus   MeSH
• DNA bakterií analýza   chemie   genetika   MeSH
• Enterococcus klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• Helix (hlemýždi) mikrobiologie   MeSH
• hybridizace nukleových kyselin MeSH
• pohyb MeSH
• RNA ribozomální 16S genetika   MeSH
• RNA transferová genetika   MeSH
• sekvenční analýza DNA MeSH
• střeva mikrobiologie   MeSH
• techniky typizace bakterií MeSH
• zastoupení bazí MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• biologické pigmenty MeSH
• DNA bakterií MeSH
• RNA ribozomální 16S MeSH
• RNA transferová MeSH

• Klíčová slova
• CHROMATOGRAPHY *, GASES *, INTESTINES *,
• MeSH
• chromatografie plynová * MeSH
• chromatografie * MeSH
• gastrointestinální obsah * MeSH
• plyny * MeSH
• střeva * MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• plyny * MeSH

• Klíčová slova
• INTESTINES/effect of radiations on *, ROENTGEN RAYS/injurious effects *,
• MeSH
• rentgenové záření škodlivé účinky   MeSH
• střeva účinky záření   MeSH
• Publikační typ
• časopisecké články MeSH