Interferon gamma (IFNγ) is a cytokine and an immunochemical marker that can be used for revealing of infectious diseases and especially for distinguishing of viral and some types of bacterial infections. Blood tests for IFNγ are typically based on immunoassays like Enzyme-Linked Immunosorbent Assay (ELISA). In this paper, a biosensor working on the principle of quartz crystal microbalance (QCM) was developed as an alternative to the standard analytical methods for IFNγ. The biosensor contained antibodies against IFNγ immobilized on QCM and also on gold nanoparticles. A sandwich containing QCM, gold nanoparticles and IFNγ was formed and formation of the sandwich caused decrease of oscillation frequency. The assay exerted limit of detection 5.7 pg/ml for a sample sized 50 μl and one measuring cycle was finished within 90 min. The assay by biosensor fully correlated to standard ELISA. In a conclusion, the biosensor appears to be a fully applicable analytical tool for a simple assay of IFNγ. Overall simplicity and no special requirement on staff and equipment are the major advantages of the here presented assay.

• Klíčová slova
• Bioassay, Biosensor, Cytokine, Diagnosis, ELISA, Immunoassay, Immunosensor, Infection, Lymphocyte, Macrophage, Virus,
• MeSH
• biosenzitivní techniky * MeSH
• imunoanalýza MeSH
• interferon gama MeSH
• kovové nanočástice * MeSH
• křemen MeSH
• lidé MeSH
• mikrorovnovážné techniky křemenného krystalu MeSH
• zlato MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• interferon gama MeSH
• křemen MeSH
• zlato MeSH

The intracellular growth of Legionella pneumophila in WiREF (Wistar rat embryonal fibroblast) cells was inhibited by porcine interferon-gamma. The effect was compared with that of different human interferons (alpha and gamma). The growth inhibition was dose-dependent and required the pretreatment of WiREF cells with interferon. The development of an antibacterial state of the cells was observed. When interferon was added together with bacteria or 1 d after the infection there was no inhibition. Also, there was no direct antibacterial effect of the interferon. In addition, cell pretreatment with a combination of interferon and antibiotics failed to show a synergistic effect.

• MeSH
• antibakteriální látky aplikace a dávkování   farmakologie   MeSH
• buněčné dělení účinky léků   MeSH
• buněčné linie MeSH
• elektronová mikroskopie MeSH
• interferon gama aplikace a dávkování   farmakologie   MeSH
• interferon typ I aplikace a dávkování   farmakologie   MeSH
• krysa rodu Rattus MeSH
• Legionella pneumophila účinky léků   růst a vývoj   ultrastruktura   MeSH
• lékové interakce MeSH
• lidé MeSH
• prasata MeSH
• rekombinantní proteiny MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• antibakteriální látky MeSH
• interferon gama MeSH
• interferon typ I MeSH
• rekombinantní proteiny MeSH

The signal transducers and transcription activators (STATs) and their endogenous inhibitors of the suppressors of cytokine signalling (SOCS) family are major proteins harmonizing the transmission of external signals from the surface membrane to target genes in the nucleus. To correlate the induction of SOCS 3 by interferons (IFNs) on messenger RNA and protein levels with STAT 1 phosphorylation in human malignant melanoma cell lines, we used a unique collection of 18 established malignant melanoma cell lines and six human non-malignant normal cells (two melanocytes, two skin keratinocytes and two fibroblasts). IFN-gamma induced SOCS 3 in 83% of melanoma cell lines, whereas IFN-alpha stimulated SOCS 3 expression in only 11% of cases. Similarly, melanocytes showed strong induction of SOCS 3 by IFN-gamma and, to a lesser extent, by IFN-alpha. In most cases, SOCS 3 expression was paralleled by STAT 1 phosphorylation at tyrosine residues (Y701). In several lines, however, SOCS 3 was not induced despite STAT 1 phosphorylation and, in a few lines, SOCS 3 induction occurred without detectable STAT 1 phosphorylation, indicating that STAT 1 might not be an exclusive inducer of SOCS 3. Similarly, non-malignant cells displayed STAT 1 activation and high levels of SOCS 3 expression after IFN-gamma (but not IFN-alpha) treatment. In conclusion, in contrast to IFN-alpha, IFN-gamma appeared to induce SOCS 3 apparently at the transcription level and exhibited higher cytotoxic effects regardless of the cell origin.

• MeSH
• buňky - růstové procesy účinky léků   MeSH
• fosforylace MeSH
• interferon gama farmakologie   MeSH
• interferon typ I farmakologie   MeSH
• lidé MeSH
• melanom farmakoterapie   genetika   metabolismus   MeSH
• messenger RNA biosyntéza   genetika   MeSH
• nádorové buněčné linie MeSH
• northern blotting MeSH
• protein SOCS3 MeSH
• proteiny SOCS biosyntéza   genetika   MeSH
• rekombinantní proteiny MeSH
• signální transdukce MeSH
• transkripční faktor STAT1 metabolismus   MeSH
• western blotting MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• interferon gama MeSH
• interferon typ I MeSH
• messenger RNA MeSH
• protein SOCS3 MeSH
• proteiny SOCS MeSH
• rekombinantní proteiny MeSH
• SOCS3 protein, human MeSH Prohlížeč
• STAT1 protein, human MeSH Prohlížeč
• transkripční faktor STAT1 MeSH

Spleen cells from BALB/c mice immunized with human leukocyte interferon gamma (HuIFN-gamma) were fused with mouse NSO myeloma cells. Nine hybridoma lines were found secreting monoclonal antibodies (MoAb) which were able to neutralize the antiviral activity of HuIFN-gamma. All nine MoAb inhibited also the antiproliferative activity of HuIFN-gamma. The ability of tested MoAb to inhibit both the antiviral and antiproliferative activities of HuIFN-gamma supports the suggestion that a common domain on HuIFN-gamma molecule might be responsible for both biological activities. However, ELISA and/or RIA proved unsuitable for detection of these specific MoAb.

• MeSH
• antivirové látky antagonisté a inhibitory   MeSH
• buněčné dělení účinky léků   MeSH
• buněčné linie MeSH
• interferon gama antagonisté a inhibitory   imunologie   farmakologie   MeSH
• lidé MeSH
• monoklonální protilátky * MeSH
• myši MeSH
• neutralizační testy MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antivirové látky MeSH
• interferon gama MeSH
• monoklonální protilátky * MeSH

Spleen cells from newborn mice are immunologically nonreactive and do not respond by proliferation upon stimulation with the T cell mitogen concanavalin-A (Con-A) or with recombinant interleukin-2 (IL-2). We have found that, in spite of the observed non-reactivity in the proliferative tests, cells from newborn mice were able to synthesize a significant level of mRNA for gamma-interferon (gamma-IFN) after stimulation with IL-2, but did not synthesize gamma-IFN upon stimulation with Con-A. Since gamma-IFN is of prime importance for antiviral and fungicidal activities and has complex regulatory functions for the cells of the immune system, we suggest that it could play an important role in the survival of newborns.

• MeSH
• aktivace lymfocytů MeSH
• inbrední kmeny myší MeSH
• interferon gama genetika   MeSH
• interleukin-2 farmakologie   MeSH
• konkanavalin A farmakologie   MeSH
• messenger RNA genetika   metabolismus   MeSH
• myši MeSH
• novorozená zvířata MeSH
• regulace genové exprese účinky léků   imunologie   MeSH
• slezina cytologie   imunologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• interferon gama MeSH
• interleukin-2 MeSH
• konkanavalin A MeSH
• messenger RNA MeSH

Recurrent aphthous stomatitis (RAS) is the most common oral ulcerative inflammatory disease with unknown etiology. IL-2 and IFN-γ are secreted by Th1 cells and the elevated levels of them have been reported in RAS. Single nucleotide polymorphisms (SNPs) of IL-2 and IFN-γ genes could alter the cytokine production. The aim of this study was to investigate frequencies of IL-2 and IFN-γ alleles and genotypes in a group of patients with minor-RAS (MiRAS). PCR-SSP method used to type genomic DNA of 64 Iranian patients with MiRAS for IL-2 gene (G -330 T) and (G +166 T) and IFN-γ gene at position UTR5644 (A/T). Frequency of each allele and genotype was compared with control group. IL-2 +166 G allele was significantly lower among patients which was reflected in significantly decreased of GG genotype at this position, while IL-2 +166 T allele was significantly higher among patients, IL-2 GT genotype was also significantly higher in RAS patients. No significant differences were found regarding IL-2 -330 G/T allele frequencies, while IL-2 GT genotype at this position was significantly higher among patients and IL-2 -330 TT genotype was significantly lower among RAS patients. Although no significant differences were found in IFN-γ allele frequencies at UTR5644 (A/T), AT genotype at this position was significantly overrepresented among patients compared with controls. Results of this study suggest that certain SNPs of IL-2 and IFN-γ genes have association with predisposition of individuals to RAS. More studies in different ethnic groups are needed to confirm results of this study.

• Klíčová slova
• Interferon-gamma, Interleukin-2, Recurrent aphthous stomatitis, Single nucleotide polymorphisms,
• MeSH
• aftózní stomatitida genetika   MeSH
• alely MeSH
• genetická predispozice k nemoci MeSH
• genotyp MeSH
• interferon gama genetika   MeSH
• interleukin-2 genetika   MeSH
• jednonukleotidový polymorfismus * MeSH
• lidé MeSH
• studie případů a kontrol MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Írán MeSH
• Názvy látek
• interferon gama MeSH
• interleukin-2 MeSH

BACKGROUND: Our retrospective study included a cohort of 47 patients who underwent living donor kidney transplantation.The pre-transplant frequencies of donor-specific Interferon-gamma (IFN-γ) producing cells were define dusing enzyme-linked immunosorbent spot (ELISpot) assay and correlated with incidence of acute cellular(ACR), antibody-mediated rejection (AMR) and kidney graft survival up to one year after transplantation. RESULTS: We found a statistically significant correlation between the frequencies of IFN-γ-producing cells and the number of mismatches in HLA antigens between patients and their respective donors – for Class I – A and B (r = 0.399, p b 0.01) and for Class I and Class II antigens – A, B and DR (r = 0.409, p b 0.01). No significant relationship was observed between the numbers of IFN-γ-secreting cells and incidence of acute rejection (neither ACR, nor AMR). However, there was a trend of elevated frequencies of IFN-γ-producing cells in patients who developed ACR or AMR in comparison with kidney recipients free of rejection (91 ± 82 and 114 ± 75 vs. 72 ± 70/5 × 10(4) peripheral blood mononuclear cells respectively). Patients with concurrent acute cellular and antibody-mediated rejection had also higher numbers of IFN-γ-producing memory/effector cells compared to patients with cellular rejection only. CONCLUSION: Pre-transplant determination of the numbers of IFN-γ-producing donor-specific memory cells using the ELISpot technique may provide clinically relevant results when evaluating the risk of development of acute cellular and antibody-mediated rejection. These frequencies are influenced by the degree of HLA mismatching between patients and their respective kidney donors.

• Klíčová slova
• Acute rejection, ELISpot, HLA antigens, Interferon-gamma, Kidney transplantation,
• MeSH
• akutní nemoc MeSH
• buněčná cytotoxicita závislá na protilátkách MeSH
• dospělí MeSH
• ELISPOT MeSH
• HLA antigeny imunologie   MeSH
• interferon gama metabolismus   MeSH
• kohortové studie MeSH
• kultivované buňky MeSH
• leukocyty mononukleární imunologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• následné studie MeSH
• přežívání štěpu MeSH
• rejekce štěpu diagnóza   imunologie   MeSH
• retrospektivní studie MeSH
• senioři MeSH
• transplantace ledvin * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• HLA antigeny MeSH
• interferon gama MeSH

BACKGROUND: Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells. AIMS: To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon gamma and E. coli derived lipopolysaccharide. RESULTS: Stimulation of J774 cells either by interferon-gamma or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection. CONCLUSIONS: Stimulation of J774 cell line by combination of interferon-gamma with lipopolysaccharide inhibits the intracellular growth of F. tularensis.

• MeSH
• buněčné linie MeSH
• Francisella tularensis růst a vývoj   MeSH
• interferon gama farmakologie   MeSH
• lipopolysacharidy farmakologie   MeSH
• makrofágy účinky léků   mikrobiologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• rekombinantní proteiny MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• interferon gama MeSH
• lipopolysacharidy MeSH
• rekombinantní proteiny MeSH

Recombinant ligands derived from small protein scaffolds show promise as robust research and diagnostic reagents and next generation protein therapeutics. Here, we derived high-affinity binders of human interferon gamma (hIFNγ) from the three helix bundle scaffold of the albumin-binding domain (ABD) of protein G from Streptococcus G148. Computational interaction energy mapping, solvent accessibility assessment, and in silico alanine scanning identified 11 residues from the albumin-binding surface of ABD as suitable for randomization. A corresponding combinatorial ABD scaffold library was synthesized and screened for hIFNγ binders using in vitro ribosome display selection, to yield recombinant ligands that exhibited K(d) values for hIFNγ from 0.2 to 10 nM. Molecular modeling, computational docking onto hIFNγ, and in vitro competition for hIFNγ binding revealed that four of the best ABD-derived ligands shared a common binding surface on hIFNγ, which differed from the site of human IFNγ receptor 1 binding. Thus, these hIFNγ ligands provide a proof of concept for design of novel recombinant binding proteins derived from the ABD scaffold.

• MeSH
• bakteriální proteiny chemie   genetika   metabolismus   MeSH
• genová knihovna MeSH
• interferon gama metabolismus   MeSH
• katalytická doména MeSH
• lidé MeSH
• molekulární modely MeSH
• mutace MeSH
• rekombinantní proteiny chemie   genetika   metabolismus   MeSH
• sérový albumin metabolismus   MeSH
• Streptococcus chemie   genetika   metabolismus   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• IgG Fc-binding protein, Streptococcus MeSH Prohlížeč
• interferon gama MeSH
• rekombinantní proteiny MeSH
• sérový albumin MeSH

Hematopoietic stem cell transplantation (HSCT) is a frequent therapeutic approach to restore hematopoiesis in patients with hematologic diseases. Patients receive a hematopoietic stem cell (HSC)-enriched donor cell infusion also containing immune cells, which may have a beneficial effect by eliminating residual neoplastic cells. However, the effect that donor innate immune cells may have on the donor HSCs has not been deeply explored. Here, we evaluate the influence of donor natural killer (NK) cells on HSC fate, concluded that NK cells negatively affect HSC frequency and function, and identified interferon-gamma (IFNγ) as a potential mediator. Interestingly, improved HSC fitness was achieved by NK cell depletion from murine and human donor infusions or by blocking IFNγ activity. Thus, our data suggest that suppression of inflammatory signals generated by donor innate immune cells can enhance engraftment and hematopoietic reconstitution during HSCT, which is particularly critical when limited HSC numbers are available and the risk of engraftment failure is high.

• Klíčová slova
• C/EBPgamma, hematopoietic stem cell, hematopoietic stem cell transplantation, interferon-gamma, natural killer cell,
• MeSH
• buňky NK imunologie   metabolismus   MeSH
• dárci tkání * MeSH
• hematopoetické kmenové buňky imunologie   metabolismus   MeSH
• interferon gama genetika   imunologie   metabolismus   MeSH
• kokultivační techniky MeSH
• kultivované buňky MeSH
• lidé MeSH
• lymfocytární deplece metody   MeSH
• myši inbrední C57BL MeSH
• myši inbrední NOD MeSH
• myši knockoutované MeSH
• myši SCID MeSH
• myši transgenní MeSH
• myši MeSH
• přežívání štěpu genetika   imunologie   MeSH
• proteiny vázající zesilovač transkripce CCAAT genetika   imunologie   metabolismus   MeSH
• stanovení celkové genové exprese metody   MeSH
• transplantace hematopoetických kmenových buněk metody   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• Cebpg protein, mouse MeSH Prohlížeč
• interferon gama MeSH
• proteiny vázající zesilovač transkripce CCAAT MeSH