Increasing attention has been dedicated to trace phthalates in bottled water due to the serious concerns on public health, while there is still a lack of systematic analysis and assessment of current global situation. Through analyzing five representative phthalates in bottled water over 20 countries, this work clearly revealed the phthalates-associated potential risks in both human daily intake and estrogenic effect. In the risk assessment, the kinetic models were also developed to describe and predict phthalates migration. In more than three hundred brands of bottled waters from twenty one countries, the detection frequency of the five targeted phthalates was found to be in the order of dibutyl phthalate (DBP, 67.6%), di-2-(ethyl hexyl) phthalate (DEHP, 61.7%), diethyl phthalate (DEP, 47.1%), benzyl butyl phthalate (BBP, 36.9%), and dimethyl phthalate (DMP, 30.1%). Among the countries studied relating concentrations of DEHP in bottled waters, the top five countries ranked in the order of high to low were Thailand, Croatia, Czech Republic, Saudi Arabia and China with an average level of 61.1, 8.8, 6.3, 6.2 and 6.1 μg/L, respectively. The average levels of BBP, DBP, DMP and DEP in bottled water from Pakistan were high, in which DEP and DMP were ranked 1st among all countries with the average levels of 22.4 and 50.2 μg/L, while BBP and DBP were ranked 2nd and 3rd with the average levels of 7.5 and 17.8 μg/L, respectively. The human daily intake-based risk assessment revealed that phthalates in bottled waters studied would not pose a serious concern on public health. However, the adverse estrogenic effects of phthalates in bottled water from some countries appeared to be significant. This study just shed light on global situation of phthalates in bottled water, and more efforts should be needed to systematically examine the phthalates-related safety of bottled water.

• Klíčová slova
• Bottled water, Daily intakes, Estrogenic effects, Migration kinetics, Phthalates, Risk assessment,
• MeSH
• kyseliny ftalové * MeSH
• lidé MeSH
• pitná voda * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Geografické názvy
• Česká republika MeSH
• Chorvatsko MeSH
• Čína MeSH
• Pákistán MeSH
• Saudská Arábie MeSH
• Thajsko MeSH
• Názvy látek
• kyseliny ftalové * MeSH
• phthalic acid MeSH Prohlížeč
• pitná voda * MeSH

Being an obligate parasite, juvenile common cuckoos Cuculus canorus are thought to reach their African wintering grounds from Palearctic breeding grounds without guidance from experienced conspecifics but this has not been documented. We used satellite tracking to study naïve migrating common cuckoos. Juvenile cuckoos left breeding sites in Finland moving slowly and less consistently directed than adult cuckoos. Migration of the juveniles (N = 5) was initiated later than adults (N = 20), was directed toward the southwest-significantly different from the initial southeast direction of adults-and included strikingly long Baltic Sea crossings (N = 3). After initial migration of juvenile cuckoos toward Poland, the migration direction changed and proceeded due south, directly toward the winter grounds, as revealed by a single tag transmitting until arrival in Northwest Angola where northern adult cuckoos regularly winter. Compared to adults, the juvenile travelled straighter and faster, potentially correcting for wind drift along the route. That both migration route and timing differed from adults indicates that juvenile cuckoos are able to reach proper wintering grounds independently, guided only by their innate migration programme.

• MeSH
• časové faktory MeSH
• migrace zvířat * MeSH
• pohlavní dospělost MeSH
• ptáci růst a vývoj   fyziologie   MeSH
• satelitní přenosy * MeSH
• vítr MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Angola MeSH
• Finsko MeSH

Animal migration has fascinated scientists and the public alike for centuries, yet migratory animals are facing diverse threats that could lead to their demise. The Anthropocene is characterised by the reality that humans are the dominant force on Earth, having manifold negative effects on biodiversity and ecosystem function. Considerable research focus has been given to assessing anthropogenic impacts on the numerical abundance of species/populations, whereas relatively less attention has been devoted to animal migration. However, there are clear linkages, for example, where human-driven impacts on migration behaviour can lead to population/species declines or even extinction. Here, we explore anthropogenic threats to migratory animals (in all domains - aquatic, terrestrial, and aerial) using International Union for the Conservation of Nature (IUCN) Threat Taxonomy classifications. We reveal the diverse threats (e.g. human development, disease, invasive species, climate change, exploitation, pollution) that impact migratory wildlife in varied ways spanning taxa, life stages and type of impact (e.g. from direct mortality to changes in behaviour, health, and physiology). Notably, these threats often interact in complex and unpredictable ways to the detriment of wildlife, further complicating management. Fortunately, we are beginning to identify strategies for conserving and managing migratory animals in the Anthropocene. We provide a set of strategies that, if embraced, have the potential to ensure that migratory animals, and the important ecological functions sustained by migration, persist.

• Klíčová slova
• animal movement, biodiversity, conservation, natural resources management, phenology, wildlife biology,
• MeSH
• biodiverzita MeSH
• ekosystém MeSH
• klimatické změny MeSH
• lidé MeSH
• lidské činnosti MeSH
• migrace zvířat * MeSH
• zachování přírodních zdrojů * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH

BACKGROUND: The expression of aquaporin 4 (AQP4) and intermediate filament (IF) proteins is altered in malignant glioblastoma (GBM), yet the expression of the major IF-based cytolinker, plectin (PLEC), and its contribution to GBM migration and invasiveness, are unknown. Here, we assessed the contribution of plectin in affecting the distribution of plasmalemmal AQP4 aggregates, migratory properties, and regulation of cell volume in astrocytes. METHODS: In human GBM, the expression of glial fibrillary acidic protein (GFAP), AQP4 and PLEC transcripts was analyzed using publicly available datasets, and the colocalization of PLEC with AQP4 and with GFAP was determined by immunohistochemistry. We performed experiments on wild-type and plectin-deficient primary and immortalized mouse astrocytes, human astrocytes and permanent cell lines (U-251 MG and T98G) derived from a human malignant GBM. The expression of plectin isoforms in mouse astrocytes was assessed by quantitative real-time PCR. Transfection, immunolabeling and confocal microscopy were used to assess plectin-induced alterations in the distribution of the cytoskeleton, the influence of plectin and its isoforms on the abundance and size of plasmalemmal AQP4 aggregates, and the presence of plectin at the plasma membrane. The release of plectin from cells was measured by ELISA. The migration and dynamics of cell volume regulation of immortalized astrocytes were assessed by the wound-healing assay and calcein labeling, respectively. RESULTS: A positive correlation was found between plectin and AQP4 at the level of gene expression and protein localization in tumorous brain samples. Deficiency of plectin led to a decrease in the abundance and size of plasmalemmal AQP4 aggregates and altered distribution and bundling of the cytoskeleton. Astrocytes predominantly expressed P1c, P1e, and P1g plectin isoforms. The predominant plectin isoform associated with plasmalemmal AQP4 aggregates was P1c, which also affected the mobility of astrocytes most prominently. In the absence of plectin, the collective migration of astrocytes was impaired and the dynamics of cytoplasmic volume changes in peripheral cell regions decreased. Plectin's abundance on the plasma membrane surface and its release from cells were increased in the GBM cell lines. CONCLUSIONS: Plectin affects cellular properties that contribute to the pathology of GBM. The observed increase in both cell surface and released plectin levels represents a potential biomarker and therapeutic target in the diagnostics and treatment of GBMs.

• Klíčová slova
• Aquaporin 4, Astrocyte, Cell migration, Cell volume, Cytoskeleton, Edema, Glioblastoma, Intermediate filaments, Plectin,
• MeSH
• akvaporin 4 MeSH
• astrocyty MeSH
• biologické markery MeSH
• glioblastom * MeSH
• lidé MeSH
• myši MeSH
• plektin MeSH
• protein - isoformy MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• akvaporin 4 MeSH
• biologické markery MeSH
• Plec protein, mouse MeSH Prohlížeč
• plektin MeSH
• protein - isoformy MeSH

Every year, many wild animals undertake long-distance migration to breed in the north, taking advantage of seasonally high pulses in food supply, fewer parasites, and lower predation pressure in comparison with equatorial latitudes. Growing evidence suggests that climate-change-induced phenological mismatches have reduced food availability. Furthermore, novel pathogens and parasites are spreading northwards, and nest or offspring predation has increased at many Arctic and northern temperate locations. Altered trophic interactions have decreased the reproductive success and survival of migratory animals. Reduced advantages for long-distance migration have potentially serious consequences for community structure and ecosystem function. Changes in the benefits of migration need to be integrated into projections of population and ecosystem dynamics and targeted by innovative conservation actions.

• Klíčová slova
• climate change, food supply, nest predation, parasites, population dynamics, trophic interactions,
• MeSH
• ekosystém * MeSH
• klimatické změny MeSH
• migrace zvířat * MeSH
• predátorské chování MeSH
• roční období MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Geografické názvy
• Arktida MeSH

Proper course of folliculogenesis and oogenesis have an enormous impact on female fertility. Both processes take place in the ovary and involve not only the maturing germ cell, but also few types of somatic cells that assist the ovarian processes and mediate the dialog with the oocyte. These cells, granulosa and theca, are heavily involved in essential reproductive processes, such as ovulation, fertilization, and embryo implantation. In this study, we have used the expressive microarray approach to analyze the transcriptome of porcine granulosa cells, during short-term in vitro culture. We have further selected differentially expressed gene ontologies, involved in cell proliferation, migration, adhesion, and tissue development, namely, "cell-cell adhesion," "cell motility," "cell proliferation," "tissue development," and "tissue migration" to screen them for the possibility of discovery of new markers of those processes. A total of 303 genes, expression of which varied significantly in different culture periods and belonged to the analyzed ontology groups, were detected, of which 15 that varied the most (between 0 and 48 h of culture) were selected for validation. As the validation confirmed the transcriptomic patterns, 10 genes of biggest changes in expression (CAV1, IGFBP5, ITGB3, FN1, ITGA2, LAMB1, POSTN, FAM83D, KIF14, and CDK1) were analyzed, described, and referred to the context of the study, with the most promising new markers and further proof for the viability of the currently recognized ones detailed. Overall, the study provided valuable insight into the molecular functioning of in vitro granulosa cell cultures.

• Klíčová slova
• Maturation (IVM), adhesion, cellular migration, porcine granulosa cells, proliferation,
• MeSH
• biologické markery metabolismus   MeSH
• buněčná adheze genetika   MeSH
• folikulární buňky cytologie   metabolismus   fyziologie   MeSH
• kultivované buňky MeSH
• pohyb buněk genetika   MeSH
• prasata MeSH
• proliferace buněk genetika   MeSH
• sekvenční analýza hybridizací s uspořádaným souborem oligonukleotidů MeSH
• stanovení celkové genové exprese MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biologické markery MeSH

Primordial germ cells (PGCs) arise elsewhere in the embryo and migrate into developing gonadal ridges during embryonic development. In several model animals, formation and migration patterns of PGCs have been studied, and it is known that these patterns vary. Sturgeons (genus Acipenser) have great potential for comparative and evolutionary studies of development. Sturgeons belong to the super class Actinoptergii, and their developmental pattern is similar to that of amphibians, although their phylogenetic position is an out-group to teleost fishes. Here, we reveal an injection technique for sturgeon eggs allowing visualization of germplasm and PGCs. Using this technique, we demonstrate that the PGCs are generated at the vegetal pole of the egg and they migrate on the yolky cell mass toward the gonadal ridge. We also provide evidence showing that PGCs are specified by inheritance of maternally supplied germplasm. Furthermore, we demonstrate that the migratory mechanism is well-conserved between sturgeon and other remotely related teleosts, such as goldfish, by a single PGCs transplantation (SPT) assay. The mode of PGCs specification in sturgeon is similar to that of anurans, but the migration pattern resembles that of teleosts.

• MeSH
• biologické modely * MeSH
• embryo nesavčí cytologie   embryologie   MeSH
• embryonální vývoj fyziologie   MeSH
• pohyb buněk fyziologie   MeSH
• ryby embryologie   MeSH
• zárodečné buňky cytologie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Colon cancer is the most common type of gastrointestinal cancer. Despite advances during the last two decades, the efficacy of colorectal cancer treatment is still insufficient and new anticancer agents are necessary. METHODS: In our study, colon cancer cells derived from a primary tumor (SW480) and lymph node metastasis (SW620) from the same patient were used and compared. The effect of flubendazole (FLU) on cell adhesion and migration was monitored using the x-CELLigence Real-Time Cell Analysis system. Expressions of molecules involved in adhesion and migration were analyzed using RT-PCR and western blot. Furthermore, RNA silencing of nuclear factor-κB in SW620 cells was used to determine the involvement of the NF-κB p65 regulation pathway in FLU action. RESULTS: FLU significantly suppressed the adhesion of SW480 cells and reduced the expression of adhesion markers (ICAM-1, αE-catenin; β-catenin; integrin α5 and β1). Moreover, a significant anti-migratory potential of FLU was manifested in the SW620 cells. In addition, FLU suppressed the phosphorylation of NF-κB p65 and potentiated the suppression of several metastatic markers (ICAM-1, EpCAM, integrin α5, β1, α-tubulin) caused by NF-κB p65 silencing. CONCLUSION: FLU has a significant anti-migratory effect in intestinal cancer cell SW480 and its lymph node metastatic cells SW620. FLU decreases the expression of some proteins involved in metastatic processes and inhibits activation of NF-κB p65.

• Klíčová slova
• Colon cancer, RNA silencing, adhesion, flubendazole, metastasis, migration.,
• MeSH
• antitumorózní látky chemie   farmakologie   MeSH
• buněčná adheze účinky léků   MeSH
• léky antitumorózní - screeningové testy MeSH
• lidé MeSH
• mebendazol analogy a deriváty   chemie   farmakologie   MeSH
• molekulární struktura MeSH
• nádorové buňky kultivované MeSH
• nádory tračníku farmakoterapie   patologie   MeSH
• pohyb buněk účinky léků   MeSH
• proliferace buněk účinky léků   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• vztahy mezi strukturou a aktivitou MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antitumorózní látky MeSH
• flubendazole MeSH Prohlížeč
• mebendazol MeSH

Migration and invasiveness are phenotypic characteristics of cells that contribute to physiological processes, such as wound healing or embryogenesis and they are involved in serious pathological processes, namely in tumor cell metastasis. Availability of methods for studying migration and invasiveness of the cells is important for understanding molecular basis of these processes. In the case of cancer, migration, invasiveness and metastatic potential of tumor cells are key factors that determine clinical prognosis of the patients. This communication provides an overview of in vitro and in vivo methods which are used to study cell migration, invasion and metastasis. In vitro meth-ods for studying cell migration include simple two dimensional assays (scratch - wound assay and the assay based on the effect of hepatocyte growth factor) and methods based on chemotaxis (Dunns chamber, videomicroscopy of cells, the use of carriers with chemoattractants). Methods for studying both cell migration and invasiveness in vitro include more complex systems based on the principle of the Boyden chamber (transwell migration/ invasive test, analysis of cell migration and invasion in xCELLigence system, confocal microscopy based approaches) as well as analysis of cell migration in microchannels. Our overview of in vivo methods provides an introduction into model organisms and methods used in this field, with an emphasis on the study of cancer metastasis in mouse models. The methods described in this review are mainly involved in larger research projects aiming at developing new diagnostic and therapeutic approaches in oncology.

• MeSH
• analýza buněčné migrace MeSH
• invazivní růst nádoru patofyziologie   MeSH
• lidé MeSH
• metastázy nádorů patofyziologie   MeSH
• pohyb buněk fyziologie   MeSH
• techniky in vitro metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH

Human anterior gradient proteins AGR2 and AGR3 are overexpressed in a variety of adenocarcinomas and are often secreted in cancer patients' specimens, which suggests a role for AGR proteins in intra and extracellular compartments. Although these proteins exhibit high sequence homology, AGR2 is predominantly described as a pro-oncogene and a potential prognostic biomarker. However, little is known about the function of AGR3. Therefore, the aim of the present study was to investigate the role of AGR3 in breast cancer. The results demonstrated that breast cancer cells secrete AGR3. Furthermore, it was revealed that extracellular AGR3 (eAGR3) regulates tumor cell adhesion and migration. The current study indicated that the pharmacological and genetic perturbation of Src kinase signaling, through treatment with Dasatinib (protein kinase inhibitor) or investigating cells that express a dominant-negative form of Src, significantly abrogated eAGR3-mediated breast cancer cell migration. Therefore, the results indicated that eAGR3 may control tumor cell migration via activation of Src kinases. The results of the present study indicated that eAGR3 may serve as a microenvironmental signaling molecule in tumor-associated processes.

• Klíčová slova
• Src family kinases, Src phosphorylation, adhesion, anterior gradient proteins, cancer, migration, secreted protein disulfide isomerase family,
• Publikační typ
• časopisecké články MeSH