Relocation
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IMPORTANCE: Overweight and obesity affect millions of children and adolescents worldwide, and its prevalence is increasing. OBJECTIVE: To investigate the associations of changes in the surrounding residential environment following relocation on childhood body mass index (BMI), focusing on 3 external exposome domains: air pollution, the built environment, and socioeconomic disadvantage. DESIGN, SETTING, AND PARTICIPANTS: This longitudinal cohort study used harmonized data from birth cohorts from the Netherlands (PIAMA), Sweden (BAMSE), and the Czech Republic (ELSPAC-CZ) participating in the EXPANSE (Exposome Powered Tools for Healthy Living in Urban Settings) project, with birth dates ranging between 1991 and 1997. Participants were youths aged 2 to 24 years who had experienced residential relocation during their follow-up. Analysis focused on within-individual changes resulting from relocation. k Means clustering characterized multiple exposures from the 3 external exposome domains. Fixed-effects linear models estimated associations of exposome changes with changes in age- and sex-standardized body mass index (z-BMI), adjusted for relevant covariates. This study was conducted between July 2023 and January 2025. EXPOSURES: Changes in 3 external exposome domains: (1) ambient air pollution from high-resolution surfaces; (2) the built environment, including green, blue, and gray spaces and light at night; and (3) area-level socioeconomic disadvantage indicators. Domain-specific exposome profiles were characterized as low-, medium-, and high-hazard environments. MAIN OUTCOME AND MEASURES: Changes in z-BMI. RESULTS: The study included 4359 participants (1467 from PIAMA, 1778 from BAMSE, and 1114 from ELSPAC-CZ). A total of 2215 (50.8%) were male. The mean (SD) age at inclusion was 3.0 (1.1) years, and mean (SD) age at moving was 7.7 (4.3) years. Parental education varied across cohorts. Mean (SD) z-BMI was 0.2 (1.1), 0.4 (1.0), and 0.1 (1.2) at baseline and 0.0 (1.0), 0.3 (1.0), and 0.1 (1.1) after moving in PIAMA, BAMSE, and ELSPAC-CZ, respectively. Moving to higher-hazard environments (more polluted, more gray space) was associated with increases in z-BMI for all domains in PIAMA; significant associations were also seen for some domains and exposures in BAMSE and ELSPAC-CZ. Specifically, an association between moving to a more built environment and increase in z-BMI was consistent across cohorts: an IQR increase in gray spaces was associated with increases of 0.04 (95% CI, 0.01-0.06) units and 0.05 (95% CI, 0.01-0.09) units in z-BMI in BAMSE and PIAMA, respectively. An IQR increase in air pollution hazard was associated with increases of 0.07 (95% CI, 0.02-0.12) units and 0.07 (95% CI, 0.01-0.14) units in z-BMI for nitrogen dioxide (NO2) and fine particulate matter (PM2.5), respectively, in PIAMA. Presence of effect modification by parental education and age at moving varied across cohorts. CONCLUSIONS AND RELEVANCE: In this multicountry cohort study of 4359 youths in the Netherlands, Sweden, and the Czech Republic, moving to greener, less urbanized environments was associated with healthy childhood BMI trajectories. Heterogeneity across cohorts highlighted the context-specific influence of external exposome domains on childhood weight.
- MeSH
- charakteristiky bydlení MeSH
- dítě MeSH
- hmotnostní křivka * MeSH
- index tělesné hmotnosti MeSH
- lidé MeSH
- longitudinální studie MeSH
- mladiství MeSH
- mladý dospělý MeSH
- obezita dětí a dospívajících * epidemiologie MeSH
- předškolní dítě MeSH
- socioekonomické faktory MeSH
- vystavení vlivu životního prostředí * škodlivé účinky MeSH
- vytvořené prostředí statistika a číselné údaje MeSH
- znečištění ovzduší * škodlivé účinky statistika a číselné údaje MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
- Nizozemsko epidemiologie MeSH
- Švédsko epidemiologie MeSH
Dual-specificity mitogen-activated protein kinases kinases (MAPKKs) are the immediate upstream activators of MAPKs. They simultaneously phosphorylate the TXY motif within the activation loop of MAPKs, allowing them to interact with and regulate multiple substrates. Often, the activation of MAPKs triggers their nuclear translocation. However, the spatiotemporal dynamics and the physiological consequences of the activation of MAPKs, particularly in plants, are still poorly understood. Here, we studied the activation and localization of the Medicago sativa stress-induced MAPKK (SIMKK)-SIMK module after salt stress. In the inactive state, SIMKK and SIMK co-localized in the cytoplasm and in the nucleus. Upon salt stress, however, a substantial part of the nuclear pool of both SIMKK and SIMK relocated to cytoplasmic compartments. The course of nucleocytoplasmic shuttling of SIMK correlated temporally with the dual phosphorylation of the pTEpY motif. SIMKK function was further studied in Arabidopsis plants overexpressing SIMKK-yellow fluorescent protein (YFP) fusions. SIMKK-YFP plants showed enhanced activation of Arabidopsis MPK3 and MPK6 kinases upon salt treatment and exhibited high sensitivity against salt stress at the seedling stage, although they were salt insensitive during seed germination. Proteomic analysis of SIMKK-YFP overexpressors indicated the differential regulation of proteins directly or indirectly involved in salt stress responses. These proteins included catalase, peroxiredoxin, glutathione S-transferase, nucleoside diphosphate kinase 1, endoplasmic reticulum luminal-binding protein 2, and finally plasma membrane aquaporins. In conclusion, Arabidopsis seedlings overexpressing SIMKK-YFP exhibited higher salt sensitivity consistent with their proteome composition and with the presumptive MPK3/MPK6 hijacking of the salt response pathway.
- Klíčová slova
- Arabidopsis, MAPK, Medicago, SIMK, SIMKK, proteomics, salt stress, subcellular relocation.,
- MeSH
- aktivace enzymů MeSH
- Arabidopsis genetika růst a vývoj metabolismus MeSH
- exprese genu MeSH
- geneticky modifikované rostliny genetika růst a vývoj metabolismus MeSH
- Medicago sativa enzymologie genetika MeSH
- mitogenem aktivované proteinkinasy kinas genetika metabolismus MeSH
- rostlinné proteiny genetika metabolismus MeSH
- semenáček genetika růst a vývoj metabolismus MeSH
- soli metabolismus MeSH
- transport proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mitogenem aktivované proteinkinasy kinas MeSH
- rostlinné proteiny MeSH
- soli MeSH
Burn wound contracture of the dorsal side of fingers often leads to retraction of the nailfold with exposure of the growth zone of the nail. Disfigured nails, extremely annoying to the patients, are the results. A surgical procedure and clinical results of 50 cases (fingers) are reported and commented on.
BACKGROUND: Resurveying historical vegetation plots has become more and more popular in recent years as it provides a unique opportunity to estimate vegetation and environmental changes over the past decades. Most historical plots, however, are not permanently marked and uncertainty in plot location, in addition to observer bias and seasonal bias, may add significant error to temporal change. These errors may have major implications for the reliability of studies on long-term environmental change and deserve closer attention of vegetation ecologists. MATERIAL & METHODS: Vegetation data obtained from the resurveying of non-permanently marked plots are assessed for their potential to study environmental-change effects on plant communities and the challenges the use of such data have to meet. We describe the properties of vegetation resurveys distinguishing basic types of plots according to relocation error, and we highlight the potential of such data types for studying vegetation dynamics and their drivers. Finally, we summarise the challenges and limitations of resurveying non-permanently marked vegetation plots for different purposes in environmental change research. RESULTS AND CONCLUSIONS: Resampling error is caused by three main independent sources of error: error caused by plot relocation, observer bias, and seasonality bias. For relocation error, vegetation plots can be divided into permanent and non-permanent plots, while the latter are further divided into quasi-permanent (with approximate relocation) and non-traceable (with random relocation within a sampled area) plots. To reduce the inherent sources of error in resurvey data, the following precautions should be followed: (i) resurvey historical vegetation plots whose approximate plot location within a study area is known; (ii) consider all information available from historical studies in order to keep plot relocation errors low; (iii) resurvey at times of the year when vegetation development is comparable to the historical survey to control for seasonal variability in vegetation; (iv) keep a high level of experience of the observers to keep observer bias low; and (v) edit and standardise datasets before analyses.
- MeSH
- akademický sbor * dějiny MeSH
- dějiny 20. století MeSH
- dějiny lékárnictví MeSH
- farmacie * MeSH
- kontinuální vzdělávání farmaceutů dějiny MeSH
- studium farmacie vysokoškolské dějiny MeSH
- studium farmacie dějiny MeSH
- univerzity dějiny MeSH
- veřejná politika MeSH
- vláda dějiny MeSH
- vládní programy výchova dějiny MeSH
- Check Tag
- dějiny 20. století MeSH
- Publikační typ
- časopisecké články MeSH
- historické články MeSH
- Geografické názvy
- Československo etnologie MeSH
There is increasing interest across European contexts in promoting active social lives in older age, and counteracting pathways and outcomes related to social isolation and loneliness for men and women in later life. This is evidenced within national and European level policy, including the 2021 Green Paper on Ageing and its concern with understanding how risks can accrue for European ageing populations in the relational sphere. Research indicates that life-course transitions can function as a source of these risks, leading to a range of potentially exclusionary impacts for the social relations of older men and women. Findings presented in this paper are drawn from the qualitative component of a larger European mixed-methods study on exclusion from social relations (GENPATH: A life course perspective on the GENdered PATHways of social exclusion in later life, and its consequences for health and well-being). We use data from 119 in-depth interviews from four jurisdictions: Austria, Czechia, Ireland and Spain. This research employed an approach that focused on capturing lived experienced insights related to relational change across the life course, the implications of these changes for multifaceted forms of exclusion from social relations and the role of gender in patterning these changes and implications. We focused on transitions that commonly emerged across those jurisdictions for older people: onset of ill-health, bereavement, retirement and relocation. We found that these transitions translate into multidimensional experiences of exclusion from social relations in the lives of older men and women by constraining their social networks, support networks, social opportunities and intimate relationships.
- Klíčová slova
- Bereavement, Exclusion, Ill-health, Older adults, Relocation, Social relations,
- MeSH
- kvalitativní výzkum MeSH
- lidé MeSH
- osamocení MeSH
- senioři MeSH
- sociální izolace * MeSH
- stárnutí MeSH
- životní změny * MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The hemorrhage as a result of intravital bleeding is considered, from forensic point of view, as important sign of vital reaction of injury. However, in special cases it must be accepted that hemorrhage occurred after the death. The formation of supravital changes is evident, e.g., in organ donors whose blood circulation and pulmonary ventilation is kept after the brain death. The post-mortem origin of hemorrhages can also be seen in donors of eyeballs after enucleation made before the autopsy at Institute of Forensic Medicine and Toxicology. These hemorrhages are manifested after several hours when eyeballs were removed. Moreover, we observed the origin of hemorrhage in orbit which was caused nor by intravital bleeding neither by direct force. Its origin could not be explained nothing but postmortem propagation of the hemorrhage from the fracture of anterior fossa of the scull base. We did not find information about postmortem origin or relocation of hemorrhage of such extent in the literature. In the frame of knowledge about supravital reaction, this finding is of general importance with forensic impact. The documentation from the scene of death allowed correcting the appreciation of the mechanism of injury and traumatic process from the point of view of foreign culpability which should be considered in such case (e.g., a blow to the orbit with following fall under passing subway train).
- MeSH
- dospělí MeSH
- kraniocerebrální traumata patologie MeSH
- krvácení patologie MeSH
- lidé MeSH
- posmrtné změny * MeSH
- soudní patologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- kazuistiky MeSH
Eukaryotic organisms, including microbial members such as protists and green algae, utilize suites of transporter proteins to move essential metabolites across cell organelle membranes. Amongst these different transporter families, the mitochondrial carrier family (MCF) is one of the most diverse, encompassing essential NAD+ and ADP/ATP translocators, as well as amino acid, sugar and cofactor transporters. They are typically associated with the mitochondrial inner membrane, but some display more dynamic localization. Here, we perform a census of predicted MCF domains in the genome of the model diatom alga Phaeodactylum tricornutum, identifying a new family of three proteins (termed here and elsewhere "MCFc") with strong internal sequence conservation but limited similarity to other MCF proteins encoded in its genome. Considering both phylogenetic data and experimental localization, we posit that MCFc is widespread across algae with complex red chloroplasts alongside some primary green algae, and contains multiple subfamilies targeted to diatom mitochondria, plastids, and endomembranes. Finally, using data from Tara Oceans, we identify putative roles for MCFc in diatom cells, including a possible association of the plastid-targeted Phatr3_J46742 subfamily in cellular nitrate assimilation. Our data provide insights into the evolutionary diversification of the membrane transport mechanisms associated with diatoms and other eukaryotic algae.
- Klíčová slova
- GFP‐confocal microscopy, environmental sequencing, gene co‐regulation, plastid proteomes, solute carrier family 25,
- MeSH
- fylogeneze MeSH
- mitochondrie metabolismus MeSH
- rozsivky * metabolismus genetika MeSH
- Publikační typ
- časopisecké články MeSH
Formins are evolutionarily conserved eukaryotic proteins engaged in actin nucleation and other aspects of cytoskeletal organization. Angiosperms have two formin clades with multiple paralogs; typical plant Class I formins are integral membrane proteins that can anchor cytoskeletal structures to membranes. For the main Arabidopsis housekeeping Class I formin, FH1 (At3g25500), plasmalemma localization was documented in heterologous expression and overexpression studies. We previously showed that loss of FH1 function increases cotyledon epidermal pavement cell shape complexity via modification of actin and microtubule organization and dynamics. Here, we employ transgenic Arabidopsis expressing green fluorescent protein-tagged FH1 (FH1-GFP) from its native promoter to investigate in vivo behavior of this formin using advanced microscopy techniques. The fusion protein is functional, since its expression complements the fh1 loss-of-function mutant phenotype. Accidental overexpression of FH1-GFP results in a decrease in trichome branch number, while fh1 mutation has the opposite effect, indicating a general role of this formin in controlling cell shape complexity. Consistent with previous reports, FH1-GFP associates with membranes. However, the protein exhibits surprising actin- and secretory pathway-dependent dynamic localization and relocates between cellular endomembranes and the plasmalemma during cell division and differentiation in root tissues, with transient tonoplast localization at the transition/elongation zones border. FH1-GFP also accumulates in actin-rich regions of cortical cytoplasm and associates with plasmodesmata in both the cotyledon epidermis and root tissues. Together with previous reports from metazoan systems, this suggests that formins might have a shared (ancestral or convergent) role at cell-cell junctions.
- Klíčová slova
- Arabidopsis thaliana, At3g25500, Cytoskeleton, Endomembrane system, Plasmodesmata, Vacuole,
- MeSH
- Arabidopsis cytologie metabolismus MeSH
- cytoskelet genetika metabolismus MeSH
- kořeny rostlin cytologie metabolismus MeSH
- plazmodesmy fyziologie MeSH
- proteiny huseníčku genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- proteiny huseníčku MeSH
AIM: This study was designed to evaluate feasibility and effectiveness of hysteroscopic intervention in the management of symptoms related to the displaced levonorgestrel-releasing intrauterine system (LNG-IUS). MATERIAL AND METHODS: One hundred and thirteen patients with displaced LNG-IUS presenting with irregular uterine bleeding, pelvic pain or asymptomatic displacement were recruited for hysteroscopic examination. Displaced LNG-IUS was relocated by hysteroscopic intervention and the effect on symptoms and LNG-IUS position was followed. RESULTS: The displaced LNG-IUS was successfully relocated by hysteroscope in 112 (99.1%) of 113 cases. Following LNG-IUS relocation, 71 (79.8%) patients of 89 with preoperative irregular uterine bleeding had amenorrhea or vaginal spotting, and 14 of 15 (93.3%) patients with preoperative pelvic pain became asymptomatic. LNG-IUS expulsion was recorded in two patients 7 and 21 days after hysteroscopy. CONCLUSION: Displaced LNG-IUS can cause clinical symptoms (e.g. irregular bleeding, pain). Hysteroscopic relocation of displaced LNG-IUS is a feasible method in the management of these symptoms. Risk of spontaneous expulsion associated with hysteroscopy is low.
- MeSH
- dospělí MeSH
- hysteroskopie * MeSH
- kohortové studie MeSH
- levonorgestrel aplikace a dávkování MeSH
- lidé středního věku MeSH
- lidé MeSH
- metroragie etiologie prevence a kontrola MeSH
- migrace nitroděložního tělíska škodlivé účinky MeSH
- mladý dospělý MeSH
- následné studie MeSH
- nitroděložní tělíska hormonální škodlivé účinky MeSH
- odstranění implantátu škodlivé účinky metody MeSH
- pánevní bolest etiologie prevence a kontrola MeSH
- studie proveditelnosti MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- levonorgestrel MeSH
