OBJECTIVE: Emergence delirium (ED) is a postoperative complication in pediatric anesthesia characterized by a perception and psychomotor disorder, with a negative impact on postoperative recovery. As the use of inhalation anesthesia is associated with a higher incidence of ED, we investigated whether titrating the depth of general anesthesia with BIS monitor can reduce the incidence of ED. DESIGN: Randomized, prospective, and double-blind. SETTING: Patients undergoing endoscopic adenoidectomy under general anesthesia according to a uniform protocol. PATIENTS: A total of 163 patients of both sexes aged 3-8 years were enrolled over 18 months. INTERVENTIONS: Immediately after the induction of general anesthesia, a bispectral index (BIS) electrode was placed on the patient's forehead. In the study group, the depth of general anesthesia was monitored with the aim of achieving BIS values of 40-60. In the control group, the dose of sevoflurane was determined by the anaesthesiologist based on MAC (minimum alveolar concentration) and the end-tidal concentration. MEASUREMENTS: The primary objective was to compare the occurrence of ED during the PACU (post-anesthesia care unit) stay in both arms of the study. The secondary objective was to determine the PAED score at 10 and 30 min in the PACU and the need for rescue treatment of ED. MAIN RESULTS: 86 children were randomized in the intervention group and 77 children in the control group. During the entire PACU stay, 23.3% (38/163) of patients developed ED with PAED score >10: 35.1% (27/77) in the control group and 12.8% (11/86) in the intervention group (p = 0.001). Lower PAED scores were also found in the intervention group at 10 (p < 0.001) and 30 (p < 0.001) minutes compared to the control group. The need for rescue treatment did not differ between groups (p = 0.067). CONCLUSION: Individualization of the depth of general anesthesia with BIS monitoring is an effective method of preventing ED in children. CLINICAL TRIAL REGISTRATION: NCT04466579.

• Klíčová slova
• Children, Emergence delirium, PAED score, Postoperative outcomes, Postoperative recovery,
• MeSH
• celková anestezie * škodlivé účinky   MeSH
• dítě MeSH
• inhalační anestezie * škodlivé účinky   MeSH
• lidé MeSH
• pooperační delirium * epidemiologie   prevence a kontrola   etiologie   MeSH
• předškolní dítě MeSH
• probouzení z anestezie MeSH
• prospektivní studie MeSH
• sevofluran MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• randomizované kontrolované studie MeSH
• Názvy látek
• sevofluran MeSH

Emergence delirium (ED) is a postoperative complication in pediatric anesthesia characterized by perception and psychomotor disorder and has a negative impact on morbidity in the form of maladaptive behavior, which can last weeks after anesthesia. Patients with developed ED present with psychomotor anxiety, agitation, and are at higher risk of unintentional extraction of an intravenous cannula, self-harm and nausea and vomiting. The described incidence of ED varies between 25−80%, with a higher prevalence among children younger than 6 years of age. We aimed to determine the incidence of ED in pediatric patients (>1 month) after general anesthesia in the post-anesthesia care unit (PACU), using Paediatric Anaesthesia Emergence Delirium (PAED) score, Watcha score and Richmond agitation and sedation scale (RASS). The incidence of ED was the highest in the PAED score with cutoff ≥10 points (89.0%, n = 1088). When using PAED score >12 points, ED incidence was 19.3% (n = 236). The lowest incidence was described by Watcha and RASS scores, 18.8% (n = 230) vs. 18.1% (n = 221), respectively. The threshold for PAED ≥10 points seems to give false-positive results, whereas the threshold >12 points is more accurate. RASS scale, although intended primarily for estimation of the depth of sedation, seems to have a good predictive value for ED.

• Klíčová slova
• PAED score, RASS scale, Watcha score, emergence agitation, emergence delirium, pediatric anesthesia,
• Publikační typ
• časopisecké články MeSH

BACKGROUND: The aim of this study is to develop a population-specific reference for permanent tooth emergence among 4-15 years old Czech Republic children. The reference derived from this sample population are essential for pediatric dental diagnostics, orthodontic treatment planning, as well as anthropological and forensic applications. METHODS: In this cross-sectional epidemiological survey, dental examinations of 1,370 Czech children (696 girls (50,8%) and 674 boys (49,2%), whose parents or legal guardians all signed informed consent forms) from 11 elementary schools and kindergartens were performed in the classroom. During the examination, previously emerged permanent teeth (other than third molars), the child's age and gender were recorded. A tooth is defined as having erupted when at least any part of the crown penetrates the gingiva, and is clinically seen in the oral cavity. A logistic regression model was used to calculate the median emergence age per tooth for both genders and the total sample. The data was statistically processed (using IBM SPSS Statistics 23) and carried out at a deviation level of 0.05. The statistical significance of the differences in the emergence of permanent teeth (contralateral, intermaxillary, inter-gender differences) was evaluated by the McNemar test. RESULTS: No statistically significant differences were observed in the emergence times between right and left sides. In addition, mandibular teeth emerged earlier than the corresponding maxillary ones (with the exception of the first and second premolars). Permanent teeth were found to emerge sooner in girls. Furthermore, the greatest inter-gender difference was observed in maxillary canines. CONCLUSIONS: The new data reported can now be used as standards when assessing permanent tooth emergence of Czech children.

• Klíčová slova
• Children, Czech Republic, Emergence time, Permanent teeth,
• MeSH
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• prořezávání zubů * MeSH
• průřezové studie MeSH
• referenční hodnoty MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

One of the main goals of Artificial Life is to research the conditions for the emergence of life, not necessarily as it is, but as it could be. Artificial chemistries are one of the most important tools for this purpose because they provide us with a basic framework to investigate under which conditions metabolisms capable of reproducing themselves, and ultimately, of evolving, can emerge. While there have been successful attempts at producing examples of emergent self-reproducing metabolisms, the set of rules involved remain too complex to shed much light on the underlying principles at work. In this article, we hypothesize that the key property needed for self-reproducing metabolisms to emerge is the existence of an autocatalyzed subset of Turing-complete reactions. We validate this hypothesis with a minimalistic artificial chemistry with conservation laws, which is based on a Turing-complete rewriting system called combinatory logic. Our experiments show that a single run of this chemistry, starting from a tabula rasa state, discovers-with no external intervention-a wide range of emergent structures including ones that self-reproduce in each cycle. All of these structures take the form of recursive algorithms that acquire basic constituents from the environment and decompose them in a process that is remarkably similar to biological metabolisms.

• Klíčová slova
• Artificial chemistry, emergence, metabolisms, recursive algorithms, self-reproduction,
• Publikační typ
• časopisecké články MeSH

The cercarial emergence patterns of three species of Diplostomum (Diplostomum 'mergi', Diplostomum spathaceum and Diplostomum parviventosum) parasitizing freshwater first intermediate host Radix lagotis sampled in Most Lake, Czech Republic, were studied under various experimental conditions, i.e. field, laboratory and incubator, and seasons, i.e. spring, summer and autumn. We discovered unexpected daily periodicity-dependent species-specific emergence patterns among the three Diplostomum spp. depending on experimental conditions. At the same time, the intraspecific variation of D. spathaceum cercarial release in response to seasonal conditions was observed. We found that a complex array of mechanisms can affect Diplostomum species-specific patterns in cercarial emergence, of which behavioural characteristics of fish related to reproduction and feeding processes are considered the most important factors. This might represent a specific adaptive evolutionary mechanism to maximise transmission success while avoiding competition for host resources. Our results contribute to a better understanding of ecological and epidemiological aspects with respect to specific adaptive strategies compartmentalised among species of Diplostomum and consequences for infection risk in fish hosts.

• Klíčová slova
• Cercariae, Diplostomum, Emergence, Freshwater, Radix lagotis, Transmission, Trematoda,
• MeSH
• cerkárie MeSH
• druhová specificita MeSH
• hlemýždi parazitologie   MeSH
• periodicita MeSH
• roční období MeSH
• Trematoda * fyziologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

The emergence of cercariae from infected mollusks is considered one of the most important adaptive strategies for maintaining the trematode life cycle. Short transmission opportunities of cercariae are often compensated by periodic daily rhythms in the cercarial release. However, there are virtually no data on the cercarial emergence of bird schistosomes from freshwater ecosystems in northern latitudes. We investigated the daily cercarial emergence rhythms of the bird schistosome Trichobilharzia sp. "peregra" from the snail host Radix balthica in a subarctic lake under both natural and laboratory seasonal conditions. We demonstrated a circadian rhythm with the highest emergence during the morning hours, being seasonally independent of the photo- and thermo-period regimes of subarctic summer and autumn, as well as relatively high production of cercariae at low temperatures typical of northern environments. These patterns were consistent under both field and laboratory conditions. While light intensity triggered and prolonged cercarial emergence, the temperature had little effect on cercarial rhythms but regulated seasonal output rates. This suggests an adaptive strategy of bird schistosomes to compensate for the narrow transmission window. Our results fill a gap in our knowledge of the transmission dynamics and success of bird schistosomes under high latitude conditions that may serve as a basis for elucidating future potential risks and implementing control measures related to the spread of cercarial dermatitis due to global warming.

• Klíčová slova
• Trichobilharzia, bird schistosome, cercariae, emergence, light, temperature, trematodes,
• Publikační typ
• časopisecké články MeSH

OBJECTIVES: The aim of the study is to determine norms of polymorphic variation in the sequence of permanent teeth in Czech children in order to establish first standards applicable to individuals. Setting out such standards, derived from the population to which they will be applied, are essential for diagnoses in paediatric dentistry, orthodontic treatment planning and for anthropological application. METHODS: Dental examinations were performed on a sample of 1,370 children (696 girls and 674 boys) aged 4-15 years. All emerged permanent teeth (other than third molars), when at least some part of crown has penetrated the oral mucosa, and the child's gender were recorded. The frequency of sequence polymorphism was calculated for each gender by counting instances of absent/present and present/absent across all possible intra-arch and inter-arch tooth pairs. RESULTS: Differential frequencies of polymorphic sequences were more common in the inter-arch tooth pairs than in the intra-arch pairs. The most similar frequencies in inter-arch pairs were observed in second mandibular premolar/second maxillary premolar in both genders. However, in the mandible there was a polymorphic sequence in the first molar/central incisor pair in both genders. Furthermore, mandibular polymorphisms were more commonly observed in the sequences of canine/second premolar in girls than in boys. Additionally, canine/second premolar polymorphic sequences were found to be more common in the maxilla in both genders. CONCLUSIONS: The data presented in this article are useful in the prediction of tooth emergence sequence in individual Czech children and are important in paediatric dentistry and in orthodontic treatment.

• Klíčová slova
• Czech Republic, children, emergence time, permanent teeth, polymorphism, sequence,
• MeSH
• dentice trvalá * MeSH
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• polymorfismus genetický genetika   MeSH
• předškolní dítě MeSH
• prořezávání zubů genetika   MeSH
• průřezové studie MeSH
• sexuální faktory MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

BACKGROUND: The aim of this study was to investigate the influence of BMI on the timing of permanent tooth emergence in Czech children. OBJECTIVES: In this cross-sectional study, 1370 Czech children were examined. The age, gender, weight, height, all emerged permanent teeth (except third molars) of each child were all recorded. A tooth is defined as having emerged when at least any part of it has penetrated the gingiva. METHODS: A logistic regression model was used to calculate the median emergence age per tooth for both genders separately and BMI was used as a factor variable to detect statistically significant differences in the times of tooth emergence within pairs of BMI groups. The data were statistically processed using IBM SPSS Statistics 23 (SPSS Inc., Chicago, IL). RESULTS: Statistically significant differences were found for the following permanent teeth (using the FDI two-digit system): 13, 14, 15, 16, 17, 41, 44, 45, 46, 47, 33, 35, 37, 21, 24, 25, 26, 27 for girls and 12, 13, 14, 15, 16, 41, 43, 44, 45, 22, 25, 32, 34, 35, 36 for boys. These teeth were observed to emerge earlier in obese children. A similar correlation (although not statistically significant) was observed between the time of emergence of the remaining teeth and the BMI of the child. CONCLUSION: The data in this research highlight significant differences in emergence times of permanent teeth due to the influence of BMI in Czech children. These findings are important for dental treatment planning.

• Klíčová slova
• BMI, Czech children, Obesity, permanent teeth, tooth emergence,
• MeSH
• dentice trvalá * MeSH
• dítě MeSH
• lidé MeSH
• logistické modely MeSH
• obezita dětí a dospívajících epidemiologie   MeSH
• předškolní dítě MeSH
• prořezávání zubů fyziologie   MeSH
• průřezové studie MeSH
• sexuální faktory MeSH
• stomatologická péče statistika a číselné údaje   MeSH
• tělesná hmotnost MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH

BACKGROUND: Roots play an important role during plant growth and development, ensuring water and nutrient uptake. Understanding the mechanisms regulating their initiation and development opens doors towards root system architecture engineering. RESULTS: Here, we investigated by RNA-seq analysis the changes in gene expression in the barley stem base of 1 day-after-germination (DAG) and 10DAG seedlings when crown roots are formed. We identified 2,333 genes whose expression was lower in the stem base of 10DAG seedlings compared to 1DAG seedlings. Those genes were mostly related to basal cellular activity such as cell cycle organization, protein biosynthesis, chromatin organization, cytoskeleton organization or nucleotide metabolism. In opposite, 2,932 genes showed up-regulation in the stem base of 10DAG seedlings compared to 1DAG seedlings, and their function was related to phytohormone action, solute transport, redox homeostasis, protein modification, secondary metabolism. Our results highlighted genes that are likely involved in the different steps of crown root formation from initiation to primordia differentiation and emergence, and revealed the activation of different hormonal pathways during this process. CONCLUSIONS: This whole transcriptomic study is the first study aiming at understanding the molecular mechanisms controlling crown root development in barley. The results shed light on crown root emergence that is likely associated with a strong cell wall modification, death of the cells covering the crown root primordium, and the production of defense molecules that might prevent pathogen infection at the site of root emergence.

• Klíčová slova
• Barley (Hordeum vulgare L.), Crown roots, Emergence, Transcriptome,
• MeSH
• ječmen (rod) * genetika   růst a vývoj   metabolismus   MeSH
• kořeny rostlin * růst a vývoj   genetika   metabolismus   MeSH
• regulace genové exprese u rostlin * MeSH
• rostlinné geny MeSH
• semenáček růst a vývoj   genetika   MeSH
• stanovení celkové genové exprese MeSH
• transkriptom MeSH
• Publikační typ
• časopisecké články MeSH

Emergence is a concept widely used in the sciences, the arts, and engineering. Some effort has been made to formalize it, but it is used in various contexts with different meanings, and a unified theory of emergence is still distant. The ultimate goal of a theory of emergence should include using emergence to model, design, or predict the behavior of multiagent systems. The author proposes a formal definition of a basic type of emergence using a language-theoretic and grammar systems approach. It is shown which types of phenomena can be modeled in this sense and what the consequences are for other more complex phenomena.

• MeSH
• teoretické modely * MeSH
• umělá inteligence * MeSH
• Publikační typ
• časopisecké články MeSH