The aim of the study was to investigate the organ immunospecificity of human elastin using an enzyme-linked immunosorbent assay (ELISA). Soluble alpha-elastin has been isolated from human aorta and lung. Antibodies against human lung alpha-elastin have been raised in young male sheep. No evidence for the existence of an organ immunospecificity of human elastin has been found.

• MeSH
• elastin imunologie   MeSH
• ELISA MeSH
• lidé MeSH
• orgánová specificita MeSH
• ovce MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• elastin MeSH

The direct double antibody sandwich (DAS) type of enzyme-linked immunosorbent assay (ELISA) was used to determine the degree of serological and antigenic differences, among the three serotypes (A, B, C) of red clover necrotic mosaic virus (RCNMV). Homologous and heterologous antibody titres in the used IgGs to isolates TpM34 (serotype A), TpM48 (serotype B) and isolate No. 6 (serotype C) as determined by ELISA were 100- to 200-fold higher than by ring precipitation test. Intensity of homologous and heterologous reactions in ELISA depended on the concentration of antigen, of the IgG used for coating and of the labelled IgG. The IgG preparations used contained 50 to 100 times higher concentration of homologous (serotype-specific) than heterologous (interserotype-specific) antibodies. Such a great difference between the two antibody types accounts for a comparatively high degree of selectivity of the homologous reactions.

• MeSH
• antigeny virové analýza   MeSH
• ELISA metody   MeSH
• epitopy MeSH
• imunoglobulin G MeSH
• protilátky virové MeSH
• sérotypizace metody   MeSH
• viry mozaiky klasifikace   imunologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny virové MeSH
• epitopy MeSH
• imunoglobulin G MeSH
• protilátky virové MeSH

We are reporting a study evaluating the crossover of antigens reacting in Platelia Aspergillus (PA) enzyme-linked immunosorbent assay (ELISA) from faeces to vessels during mucositis as a possible cause of false-positivity of this test. In our series of 102 episodes of different grades of mucositis, we found strong reactivity of faeces in the PA ELISA test irrespective of the grade of mucositis, the percentage of oral food intake or the presence of total parenteral nutrition. However, none of the patients included in the study were positive in the serum (when the criterion of two samples with cut-off index of positivity [IP] > 0.5 was used).

• MeSH
• Aspergillus imunologie   izolace a purifikace   MeSH
• aspergilóza diagnóza   MeSH
• dospělí MeSH
• ELISA metody   MeSH
• falešně pozitivní reakce * MeSH
• feces mikrobiologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mukozitida komplikace   MeSH
• senioři MeSH
• senzitivita a specificita MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH

An interlaboratory study with 10 participants was performed to obtain validation and performance data for an enzyme-linked immunosorbent assay (ELISA) kit developed for quantitative gluten determination in foods. The ELISA kit used for this study is based on 2 monoclonal and 1 polyclonal antibody developed by Immunotech, a Beckman Coulter Co. This kit did not show any false positive results or cross-reactivity with oat, rice, maize, and buckwheat. The gliadin standard from the Working Group on Prolamin Analysis and Toxicity was included in the kit as reference material for calibration. All participants obtained a gliadin ELISA kit with Standard Operational Procedure and a form for recording test results. The study included 13 samples labeled as "gluten-free" and 2 samples spiked by wheat flour. Seven samples had gliadin content below the limit of quantitation (LOQ) of the method, and 1 sample exceeded the highest calibration level. Gliadin content in the range from 10 to 157 mg/kg (1st day) and from 11 to 183 mg/kg (2nd day) was found in 7 samples (including 2 spiked samples). Results of these samples were used for further statistical analysis and evaluation. The Cochran, Dixon, and Mandel statistical tests were applied for detection of outliers. The LOQ of the kit was estimated.

• MeSH
• analýza potravin metody   MeSH
• chemické techniky analytické metody   MeSH
• ELISA metody   MeSH
• Fagopyrum metabolismus   MeSH
• gliadin chemie   MeSH
• gluteny analýza   MeSH
• jedlá semena metabolismus   MeSH
• kalibrace MeSH
• kukuřice setá metabolismus   MeSH
• oves metabolismus   MeSH
• prolaminy MeSH
• reprodukovatelnost výsledků MeSH
• rostlinné proteiny metabolismus   MeSH
• rýže (rod) metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• gliadin MeSH
• gluteny MeSH
• prolaminy MeSH
• rostlinné proteiny MeSH

Such cytomegalovirus antibodies as the IgG and IgM are investigated in the present work. Investigation was carried out on 301 sera of fertile aged women (between 18 to 35 years of age) under the enzyme-linked immunosorbent assay (ELISA). Only one dilution has been made i.e. 1:40 and the final dilution was worked out from regression graph obtained from a previously titred human positive serum analysis; IgG antibodies were found in 265 cases (88%), appearing IgM antibodies in 4 of them, with the remaining 36 cases showing neither IgG nor IgM antibodies (12%). These results were compared with those obtained in other countries and the importance of the serological diagnosis to this virus was pointed out.

• MeSH
• cytomegalovirové infekce přenos   MeSH
• Cytomegalovirus imunologie   MeSH
• dospělí MeSH
• ELISA MeSH
• infekční komplikace v těhotenství přenos   MeSH
• lidé MeSH
• mladiství MeSH
• protilátky virové analýza   MeSH
• těhotenství MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• protilátky virové MeSH

Two modifications of an indirect enzyme immunoassay described here allow the discernment of chicken B and T cells when a polyclonal rabbit anti-chicken Ig antibody or a monoclonal antibody reactive with chicken IgM and IgG heavy chains is used. Comparison of both types of antibody in ELISA and in indirect immunofluorescence suggests that they can readily be exploited for detection of lymphoid cells with surface immunoglobulin markers.

• MeSH
• B-lymfocyty imunologie   MeSH
• ELISA * MeSH
• fluorescenční protilátková technika MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• kur domácí imunologie   MeSH
• monoklonální protilátky MeSH
• T-lymfocyty imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• monoklonální protilátky MeSH

Specific mumps virus antigens of three purification degrees have been prepared and their quality was evaluated in the enzyme-linked immunosorbent assay (ELISA) with specific human sera. Two enzyme immunoassays were elaborated, namely a simple sandwich method for IgG and the IgM-capture technique for IgM detection. Four different lots of mumps antigen were tested in these two ELISA systems. All antigens of corresponding purification degrees showed practically the same properties.

• MeSH
• antigeny virové chemie   MeSH
• ELISA * MeSH
• imunoglobulin G imunologie   MeSH
• imunoglobulin M imunologie   MeSH
• lidé MeSH
• protilátky virové analýza   MeSH
• virus příušnic imunologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny virové MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• protilátky virové MeSH

Detection of three isolates of red clover necrotic mosaic virus (RCNMV) representing A, B, and C serotypes was experimentally proved in 18 host plant species by enzyme-linked immunosorbent assay (ELISA). In all plant species tested, the homologous serotype reactions showed high selectivity. Individual virus serotypes could be reliably detected in the extracts of infected plants only with the homologous IgG fraction. Group specific detection of RCNMV without serotype determination was possible using the mixture of IgG directed to all virus serotypes occurring in the region of investigation. Intensity of positive reaction of optimally diluted IgG with the extracts from infected plants differed markedly from that of negative reaction and from the reaction background. The latter depended on the quality of serum used for the IgG preparation. For detection of small amounts of RCNMV, virus infectivity test on indicator plants was more sensitive than ELISA.

• MeSH
• ELISA MeSH
• Fabaceae mikrobiologie   MeSH
• imunoglobulin G MeSH
• králíci MeSH
• léčivé rostliny MeSH
• nemoci rostlin * MeSH
• protilátky virové MeSH
• rostliny mikrobiologie   MeSH
• viry mozaiky klasifikace   imunologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• imunoglobulin G MeSH
• protilátky virové MeSH

Multiple continuous-flow solid-phase peptide synthesis has been adapted for synthesis of peptides on a cellulose carrier (Whatman 3MM paper). Paper-bound synthetic peptides that represent antigenic determinants of particular proteins detected antibodies against the respective proteins in an enzyme-linked immunosorbent assay. The method is applied to the synthesis, and use in site-directed serology, of four peptides derived from the gp41 glycoprotein of HIV, the Epstein-Barr virus-determined nuclear antigen-1 and VCA proteins of the Epstein-Barr virus, and the early region of human papillomavirus type 11.

• MeSH
• celulosa MeSH
• ELISA MeSH
• epitopy imunologie   MeSH
• molekulární sekvence - údaje MeSH
• peptidy chemická syntéza   imunologie   MeSH
• protilátky analýza   MeSH
• sekvence aminokyselin MeSH
• sérologie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• celulosa MeSH
• epitopy MeSH
• peptidy MeSH
• protilátky MeSH

ELISA was used to determine the degree of serological relationship between red clover mottle (RCMV) and broad bean stain (BBSV) viruses and the optimal conditions for the differentiation of the two viruses by ELISA were established. The titres of homologous and heterologous antibodies determined by ELISA were 100- to 200-fold higher as compared with the ring precipitin test. Antigen concentrations of 0.15 and 0.075 ng/ml, respectively, could be detected. The reactions in ELISA depended on the concentration of antigen, of IgG used for coating and of conjugated IgG. In anti-RCMV IgG, the ratio of group-specific to species-specific antibody as determined by ELISA was from 1:1 to 1:2, while in anti-BBSV IgG this ratio was from 1:4--1:8. The differences between homologous and heterologous reactions in ELISA suggested a similar ratio of antigenic determinants in the two viruses.

• MeSH
• antigeny virové analýza   MeSH
• druhová specificita MeSH
• ELISA MeSH
• imunologická odpověď na dávku MeSH
• rostlinné viry klasifikace   imunologie   MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny virové MeSH