The protecting effect of nerve growth factor (NGF) from hydrogen peroxide was studied on PC12 cells conditioned at 1 mM hydrogen peroxide with NGF and without NGF in comparison with cells treated with neither hydrogen peroxide nor NGF. NGF treatment of PC12 cells increased significantly the activity of catalase representing induction of free radical detoxifying mechanisms. The protection effect of NGF was reflected also on enhanced activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) in the cells.

• MeSH
• acetylcholinesterasa metabolismus   MeSH
• cholin-O-acetyltransferasa metabolismus   MeSH
• enzymová indukce účinky léků   MeSH
• feochromocytom patologie   MeSH
• katalasa metabolismus   MeSH
• krysa rodu Rattus MeSH
• nádorové buňky kultivované účinky léků   enzymologie   MeSH
• nádorové proteiny metabolismus   MeSH
• nádory nadledvin patologie   MeSH
• neurotrofní faktory farmakologie   MeSH
• peroxid vodíku farmakologie   MeSH
• volné radikály MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• acetylcholinesterasa MeSH
• cholin-O-acetyltransferasa MeSH
• katalasa MeSH
• nádorové proteiny MeSH
• neurotrofní faktory MeSH
• peroxid vodíku MeSH
• volné radikály MeSH

Endothelin-1 (ET-1) and Nerve Growth Factor (NGF) are proteins, released from cancer-ridden tissues, which cause spontaneous pain and hypersensitivity to noxious stimuli. Here we examined the electrophysiological and behavioral effects of these two agents for evidence of their interactions. Individual small-medium cultured DRG sensory neurons responded to both ET-1 (50 nM, n=6) and NGF (100 ng/ml, n=4), with increased numbers of action potentials and decreased slow K(+) currents; pre-exposure to ET-1 potentiated NGF´s actions, but not vice versa. Behaviorally, single intraplantar (i.pl.) injection of low doses of ET-1 (20 pmol) or NGF (100 ng), did not increase hindpaw tactile or thermal sensitivity, but their simultaneous injections sensitized the paw to both modalities. Daily i.pl. injections of low ET-1 doses in male rats caused tactile sensitization after 21 days, and enabled further tactile and thermal sensitization from low dose NGF, in ipsilateral and contralateral hindpaws. Single injections of 100 ng NGF, without changing the paw's tactile sensitivity by itself, acutely sensitized the ipsilateral paw to subsequent injections of low ET-1. The sensitization from repeated low ET-1 dosing and the cross-sensitization between NGF and ET-1 were both significantly greater in female than in male rats. These findings reveal a synergistic interaction between cutaneously administered low doses of NGF and ET-1, which could contribute to cancer-related pain.

• MeSH
• bolest chemicky indukované   metabolismus   MeSH
• endotelin-1 aplikace a dávkování   metabolismus   toxicita   MeSH
• fyzikální stimulace škodlivé účinky   MeSH
• hmat účinky léků   fyziologie   MeSH
• injekce subkutánní MeSH
• krysa rodu Rattus MeSH
• měření bolesti metody   MeSH
• nervový růstový faktor aplikace a dávkování   metabolismus   toxicita   MeSH
• potkani Sprague-Dawley MeSH
• vazba proteinů fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• endotelin-1 MeSH
• nervový růstový faktor MeSH

In an attempt to compare effects of different neurotrophic factors on impaired memory function, young adult naive rats were trained to find the hidden platform in the Morris water maze (3 consecutive days, eight trials/day). The fimbria-fornix was unilaterally removed by aspiration and nerve growth factor (NGF) (11 micrograms/ml and 0.5 microgram/ml; groups NGF and ngf, respectively) or basic fibroblast growth factor (bFGF) (0.2 microgram/ml, group FGF) were applied via intra-cerebroventricular infusion by the osmotic minipump (flow rate 0.5 microliter/h, 14 days). Nootropic drug Cerebrolysin (EBEWE Arzneitmittel; 2.5 ml/kg/day, group CER) was applied via intraperitoneal injection (14 days). One group was formed by the rats treated with NGF (11 micrograms/ml) and Cerebrolysin (group NGFCER). Non-lesioned and lesioned only rats served as controls (groups INT and LES). After a 14-day treatment, rats were tested using the retention test (1 day, four trials). On the next day, the rats were tested using transfer test (3 days, eight trials/day). Escape latency and length of trajectory was recorded. Groups NGF, ngf, FGF and LES were similarly impaired in their ability to retrieve the old position of the platform (retention test), as well as in their ability to navigate to the new position of the platform (transfer test). In the latter, NGF group significantly differed from lesioned animals. Groups CER and NGFCER were comparable to group INT in the retention or transfer test. It is concluded that anterograde amnesia elicited by fimbria-fornix lesion can be abbreviated by NGF and/or CER, while retrograde amnesia is absent only in rats treated by CER. No short-term influence of bFGF was found. It is suggested that biochemical systems other than the cholinergic one are involved.

• MeSH
• aminokyseliny farmakologie   MeSH
• bludiště - učení účinky léků   MeSH
• fibroblastový růstový faktor 2 farmakologie   MeSH
• hipokampus cytologie   fyziologie   MeSH
• krysa rodu Rattus MeSH
• neurotrofní faktory farmakologie   MeSH
• nootropní látky farmakologie   MeSH
• parasympatický nervový systém cytologie   účinky léků   MeSH
• poruchy paměti farmakoterapie   psychologie   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• aminokyseliny MeSH
• cerebrolysin MeSH Prohlížeč
• fibroblastový růstový faktor 2 MeSH
• neurotrofní faktory MeSH
• nootropní látky MeSH

BACKGROUND: Vascular endothelial growth factor (VEGF) is not only a potent angiogenic factor but it also promotes axonal outgrowth and proliferation of Schwann cells. The aim of the present study was to quantitatively assess reinnervation of musculocutaneous nerve (MCN) stumps using motor and primary sensory neurons after plasmid phVEGF transfection and end-to-end (ETE) or end-to-side (ETS) neurorrhaphy. The distal stump of rat transected MCN, was transfected with plasmid phVEGF, plasmid alone or treated with vehiculum and reinnervated following ETE or ETS neurorrhaphy for 2 months. The number of motor and dorsal root ganglia neurons reinnervating the MCN stump was estimated following their retrograde labeling with Fluoro-Ruby and Fluoro-Emerald. Reinnervation of the MCN stumps was assessed based on density, diameter and myelin sheath thickness of regenerated axons, grooming test and the wet weight index of the biceps brachii muscles. RESULTS: Immunohistochemical detection under the same conditions revealed increased VEGF in the Schwann cells of the MCN stumps transfected with the plasmid phVEGF, as opposed to control stumps transfected with only the plasmid or treated with vehiculum. The MCN stumps transfected with the plasmid phVEGF were reinnervated by moderately higher numbers of motor and sensory neurons after ETE neurorrhaphy compared with control stumps. However, morphometric quality of myelinated axons, grooming test and the wet weight index were significantly better in the MCN plasmid phVEGF transfected stumps. The ETS neurorrhaphy of the MCN plasmid phVEGF transfected stumps in comparison with control stumps resulted in significant elevation of motor and sensory neurons that reinnervated the MCN. Especially noteworthy was the increased numbers of neurons that sent out collateral sprouts into the MCN stumps. Similarly to ETE neurorrhaphy, phVEGF transfection resulted in significantly higher morphometric quality of myelinated axons, behavioral test and the wet weight index of the biceps brachii muscles. CONCLUSION: Our results showed that plasmid phVEGF transfection of MCN stumps could induce an increase in VEGF protein in Schwann cells, which resulted in higher quality axon reinnervation after both ETE and ETS neurorrhaphy. This was also associated with a better wet weight biceps brachii muscle index and functional tests than in control rats.

• MeSH
• dextrany MeSH
• fluoresceiny MeSH
• genetická terapie metody   MeSH
• krysa rodu Rattus MeSH
• mícha patologie   MeSH
• modely nemocí na zvířatech MeSH
• nemoci periferního nervového systému patologie   terapie   MeSH
• nervová vlákna myelinizovaná patologie   MeSH
• nervus musculocutaneus metabolismus   patologie   fyziologie   MeSH
• neurologické vyšetření MeSH
• neurony metabolismus   patologie   MeSH
• potkani Wistar MeSH
• přední končetina patofyziologie   MeSH
• regenerace nervu genetika   fyziologie   MeSH
• rhodaminy MeSH
• vaskulární endoteliální růstový faktor A biosyntéza   metabolismus   terapeutické užití   MeSH
• velikost orgánu fyziologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dextrany MeSH
• fluoresceiny MeSH
• fluoro-emerald MeSH Prohlížeč
• Fluoro-Ruby MeSH Prohlížeč
• rhodaminy MeSH
• vaskulární endoteliální růstový faktor A MeSH

The ontogenetic period of life and stress can have different effects on the nerve growth factor (NGF) in the hypothalamus. The aim of our study was to investigate the influence of two mild stressors, acute and chronic exposure to forced swim (FS) or high-light open field (HL-OF), on neurons containing NGF. Immunofluorescence staining was used to reveal the density of NGF-immunoreactive (ir) cells in the hypothalamic supraoptic nucleus (SON) in adult (postnatal day 90; P90) and aged (P720) rats. The P90 and P720 rats that were subjected to acute and chronic FS showed no differences in the density of NGF-ir neurons in the SON compared with nonstressed rats. However, a significant increase in NGF-ir cells was noted after acute but not after chronic HL-OF only in P90 rats. What is more, there were no age-related (P90 vs. P720) changes in the density of NGF-ir neurons in non-stressed and FS- or HL-OF-stressed rats. Our results indicate that acute HL-OF was the only factor inducing changes in the density of NGF-ir neurons in the SON of adult rats. This could be related to the neuroprotective role of NGF-ir cells in response to acute HL-OF. The absence of age-dependent changes in the density of NGF-ir neurons may indicate that the ageing processes in SON do not generate changes in the NGF immunoreactivity of its neurons.

• MeSH
• imunohistochemie metody   MeSH
• nervový růstový faktor metabolismus   MeSH
• neurony metabolismus   patologie   MeSH
• nucleus supraopticus metabolismus   patologie   MeSH
• plavání MeSH
• počet buněk MeSH
• potkani Wistar MeSH
• psychický stres metabolismus   MeSH
• stárnutí metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• nervový růstový faktor MeSH

This study investigates the effects of antinerve growth factor (anti-NGF) application on isolated ileal contractility in the rat. For this purpose, rats were divided into four groups. The control animals (n=8) received only intraperitoneal injection of an isotonic NaCl solution (i.p). Anti-NGF was daily administered intraperitoneally at the dose of 1 ng/g level in the first experimental group (n=8), and at doses of 10 ng/g (n=7) and 40 ng/g (n=7) in the second and third experimental groups, respectively. Seven days after the injections rats were sacrificed and ileum segments were isolated. Responses to acetylcholine (ACh) were evaluated by using standard Tyrode, double-calcium Tyrode and calcium-free Tyrode solutions. The average peak amplitude of ACh-induced contractions recorded in standard Tyrode solution was significantly decreased in all three experimental groups as compared to the control group (p 0.05). When double-calcium Tyrode solution was used as the perfusion medium, the responses to ACh were also lower in all anti-NGF applied groups as compared to its control group (p 0.05). Our results showed that the application of anti-NGF reduced the contractile responses of the rat isolated ileum apparently by decreasing the calcium influx from the extracellular medium.

• MeSH
• gastrointestinální motilita účinky léků   fyziologie   MeSH
• hladké svalstvo účinky léků   fyziologie   MeSH
• ileum účinky léků   fyziologie   MeSH
• injekce intraperitoneální MeSH
• krysa rodu Rattus MeSH
• nervový růstový faktor imunologie   MeSH
• potkani Wistar MeSH
• protilátky aplikace a dávkování   MeSH
• svalová kontrakce účinky léků   fyziologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• nervový růstový faktor MeSH
• protilátky MeSH

The focus of this study was to compare the role of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) in the regeneration of experimental skin and cartilage trauma. The role of VEGF in this process is known since decade; the NGF participation on this process has been first discussed within the spinal cord injury repair. We hypothesized that both VEGF and NGF induce angiogenesis and take part on the repair process. The angiogenesis response and the cartilage regeneration after phVEGF(165) plasmid and rat pcNGF plasmid administration were investigated using BALB/c mice. PhVEGF(165) and pcNFG were injected into the right mice ear and plain vector injection into the left ear the day before trauma. The next day, all mice were ear-punched, resulting in 2-mm diameter puncture through the center of both pinnae. In BALB/c mouse strain, a significantly faster cartilage repair was observed after phVEGF(165) and pcNGF injection into punched ear area in comparison to the control group. It has been shown that the healing process is after VEGF and NGF injection driven differentially. In case of VEGF is the cartilage wound repaired by induction of new chondrocytes differentiation. In the case of NGF, the regeneration is supported by immature leukocytes attracted into the punched area. The leukocytes induct angiogenesis so far indirectly by inflammation. The NGF-induced inflammation environment may be a part of mosaic creating the complete picture of regeneration.

• MeSH
• chondrogeneze * účinky léků   fyziologie   MeSH
• fyziologická neovaskularizace * účinky léků   fyziologie   MeSH
• genetické vektory MeSH
• hojení ran * účinky léků   fyziologie   MeSH
• krysa rodu Rattus MeSH
• kůže zranění   MeSH
• látky indukující angiogenezi aplikace a dávkování   MeSH
• modely u zvířat MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• neurotrofní faktory * aplikace a dávkování   genetika   MeSH
• plazmidy MeSH
• ušní chrupavka zranění   fyziologie   MeSH
• vaskulární endoteliální růstový faktor A * aplikace a dávkování   genetika   MeSH
• zánět metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• látky indukující angiogenezi MeSH
• neurotrofní faktory * MeSH
• vaskulární endoteliální růstový faktor A * MeSH

Metallothioneins (MTs) are metal-binding proteins that have been regarded as intrinsic factors for protecting cells and tissues from metal toxicity and oxidants. Among the three major classes of MTs, MT-III is different from other MTs because it has neuronal inhibitory activity and is only expressed in the central nervous system. Recent studies, however, have confirmed that MT-III is also expressed in organs other than the brain. These findings not only indicate that MT-III has a much wider tissue distribution than was originally thought, but also suggest that it might have other unknown activities. In the present study, we examined the human salivary and thyroid glands and demonstrated that the MT-III gene is also expressed in the salivary but not in the thyroid gland. While salivary ducts showed intense immuno-reactivity with anti-MT-III, weak immunoreactivity was observed in acinar cells. This, together with the findings that some neuromodulators (i.e. nerve growth factor, etc.) exist in the salivary gland and that MT-III may participate in the transport in renal tubules, suggest that MT-III may have other functions than cytoprotection in the salivary gland.

• MeSH
• lidé středního věku MeSH
• lidé MeSH
• metalothionein 3 MeSH
• mrtvola MeSH
• orgánová specificita MeSH
• proteiny nervové tkáně metabolismus   MeSH
• senioři MeSH
• slinné žlázy metabolismus   MeSH
• tkáňová distribuce MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• metalothionein 3 MeSH
• proteiny nervové tkáně MeSH

AIMS: By measuring the extent of cytokines secreted by human dental pulp stem cells (hDPSCs) from passages 2 through 10, the optimal passage of hDPSCs was determined. This offers a potential theoretical basis for the treatment of neurological disorders. METHOD: After isolation and culture of hDPSCs from human teeth, the morphological features of the cells were observed under an inverted microscope. hDPSCs were identified by their immunophenotypes and their multiple differentiation capability. Cytokine concentrations secreted in the supernatants at passages 2-10 were detected by ELISA. RESULTS: hDPSCs were viewed as fusiform or polygonal in shape, with a bulging cell body, homogenized cytoplasm, and a clear nucleus. Moreover, they could differentiate into neuroblasts in vitro. hDPSCs at passage 3 were positive for CD29 (91.5%), CD73 (94.8%) and CD90 (96.7%), but negative for the hematopoietic markers CD34 (0.13%). ELISA results showed that hDPSCs at passage 3 had the highest secretion levels of vascular endothelial growth factor (VEGF), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF), with the highest secretion level of Neurotrophin-3 (NT-3) being at passage 2. CONCLUSION: hDPSCs have steady biological features of stem cells and exhibit optimal proliferation potential. hDPSCs at different passages have different capacities in the secretion of VEGF, BDNF, NGF, and NT-3. In conclusion cytokines secreted by hDPSCs may prove to be appropriate in the treatment of neurological diseases.

• Klíčová slova
• brain-derived neurotrophic factor, cytokines, dental pulp stem cell, multidirectional differentiation, nerve growth factor, neurotrophin-3. immunophenotype, vascular endothelial growth factor,
• MeSH
• buněčná diferenciace * MeSH
• cytokiny * metabolismus   MeSH
• kmenové buňky * metabolismus   MeSH
• kultivované buňky MeSH
• lidé MeSH
• mozkový neurotrofický faktor metabolismus   MeSH
• nervový růstový faktor metabolismus   MeSH
• neurotrofin 3 metabolismus   MeSH
• proliferace buněk MeSH
• vaskulární endoteliální růstový faktor A metabolismus   MeSH
• zubní dřeň cytologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• cytokiny * MeSH
• mozkový neurotrofický faktor MeSH
• nervový růstový faktor MeSH
• neurotrofin 3 MeSH
• NTF3 protein, human MeSH Prohlížeč
• vaskulární endoteliální růstový faktor A MeSH

Genetically unmodified neural precursor cells (NPCs) derived from rate embryonic cortex were stimulated with Long-epidermal growth factor in vitro to form multicellular neurospheres. In our study, histology and transmission electron microscopy was for the first time utilized for analysis of three-dimensional neurospheres. Immunohistochemical phenotypization carried out on paraffin-embedded section revealed that most cells in the neurosphere expressed high levels of vimentin that is normally found in immature neural cells. Majority of glial cells expressed glial fibrillary acidic protein (GFAP) that is a specific marker for differentiated astrocytes. On the other hand, myelin basic protein (MBP), a marker of oligodendrocytes, was found only in a discrete subpopulation of glial cells. Similarly, electron microscopy recognized only rate myelin sheaths surrounding axons. Some cells displayed neuron-like morphology and were labelled with antibodies recognizing the neuronal antigens: 210 kDa neurofilaments (NF 210) and microtubule associated protein-2 (MAP-2). Surprisingly well-developed interneuronal synapses were found electronmicroscopically. The presence of synaptic vesicles was also confirmed by immunohistochemical detection of synaptophysin. Our results show that NPCs in neurospheres gradually differentiate into glial and neuronal cells.

• MeSH
• buněčná diferenciace MeSH
• buněčné dělení MeSH
• elektronová mikroskopie MeSH
• encefalitogenní základní proteiny analýza   MeSH
• epidermální růstový faktor farmakologie   MeSH
• gliový fibrilární kyselý protein analýza   MeSH
• imunohistochemie MeSH
• kmenové buňky chemie   ultrastruktura   MeSH
• krysa rodu Rattus MeSH
• kultivované buňky MeSH
• mozková kůra embryologie   MeSH
• neurony cytologie   ultrastruktura   MeSH
• potkani Wistar MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• encefalitogenní základní proteiny MeSH
• epidermální růstový faktor MeSH
• gliový fibrilární kyselý protein MeSH