Sets of 600 single sera from healthy individuals of various age and 458 paired sera from patients treated for diseases of various etiology were examined using a neutralization microtest technique employing prototype collection strains CA9, CB1-CB5, E1-E9, E11-E14, E17, E19, E24 and E26. Totally, 34,862 monotype neutralization tests were carried out in this study. In the set of single serum samples the lowest proportion of sera reacting with any of the enterovirus serotypes used was encountered in the group of youngest children up to the age of 2 years. This group of children showed also the highest proportion of sera free of type-specific antibody. Taken together, these sera reacted most frequently with serotypes CA9 and CB4. In the set of paired sera significant rises in antibody titre to one enterovirus serotype were recorded in 105 instances, in association with simultaneous nonsignificant rises against one or several other serotypes in 64 instances. In additional 12 paired sera there was evidenced a significant rise of antibody titre to more than one serotypes, combined in 8 of these with simultaneous nonsignificant rises to further serotypes. This neutralization microtest technique with a set of enterovirus serotypes is believed to represent a servicable diagnostic tool in determining the cause of enteroviral infection, in spite of the estimated 10% failure to establish the exact serotype responsible. The results yielded by the serologic examination of single serum samples are not considered as reflecting the actual seroconversion rates in the general population.

• MeSH
• coxsackie virózy mikrobiologie   MeSH
• dítě MeSH
• dospělí MeSH
• ECHO virové infekce mikrobiologie   MeSH
• enterovirus B lidský klasifikace   izolace a purifikace   MeSH
• Enterovirus klasifikace   izolace a purifikace   MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• neutralizační testy MeSH
• předškolní dítě MeSH
• sérotypizace MeSH
• věkové faktory MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• kojenec MeSH
• lidé MeSH
• mladiství MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH

A total of 260 feces samples from children with confirmed rotavirus infection collected during 1999-2002 were serotyped, using enzymoimmunoassay with VP7 specific monoclonal antibodies for G1-G4 serotypes. The serotypes were identified in 185 feces, i.e. 71.2 %. Individual serotypes occurred in 43, 2, 16 and 2 %; 8 % samples reacted with 2 type-specific monoclonal antibodies. The G1 serotype was prevalent over the whole period. The G3 type occurred with a statistically higher significance in children of up to 36 months (chi2 = 4.6, p = 0.028). In 4 children a different serotype was demonstrated in the first and second, or in the second and third stools, respectively. No dominant serotype was found in children with nosocomial infection.

• MeSH
• dítě MeSH
• dyzenterie diagnóza   virologie   MeSH
• feces virologie   MeSH
• imunoanalýza metody   MeSH
• infekce spojené se zdravotní péčí diagnóza   virologie   MeSH
• infekce získané v komunitě diagnóza   virologie   MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• prospektivní studie MeSH
• rotavirové infekce diagnóza   virologie   MeSH
• Rotavirus klasifikace   izolace a purifikace   MeSH
• sérotypizace metody   MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• novorozenec MeSH
• předškolní dítě MeSH
• Publikační typ
• časopisecké články MeSH
• multicentrická studie MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika epidemiologie   MeSH

The present study attempted to assess the levels of enteric viruses, as well as their serotypes, present in the wastewaters of the central Athens sewer of Keratsini (Greece), and in the receiving coastal sea-waters in the vicinity. From the parallel examination of 24 samples from each sampling source, during 1985, viruses were detected in 100% of wastewaters and in 87.5% of the receiving sea-waters. The virus loads in the receiving waters, 100 meters away from the polluting source, were found to be as low as 13% of the total virus content detected at the polluting source. From the identification of the recovered field-isolates it was found that in the polluting source (wastewater) 13 different serotypes of enteroviruses were present (not including untyped isolates), where as in the receiving coastal waters were found nine different serotypes, which correlate with those found in sewage effluents. The maximum levels detected from both sources were found in late Summer early Fall.

• MeSH
• lidé MeSH
• mikrobiologie vody * MeSH
• mořská voda * MeSH
• odpadní vody * MeSH
• sérotypizace MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• odpadní vody * MeSH

Streptococcus suis is an important pathogen of pigs but is also transmissible to humans, with potentially fatal consequences. Among 29 serotypes currently recognized, some are clinically and epidemiologically more important than others. This is particularly true for serotypes 2 and 14, which have a large impact on pig production and also on human health. Conventional PCR-based serotyping cannot distinguish between serotype 1/2 and serotype 2 or between serotype 1 and serotype 14. Although serotype 1/2 and serotype 2 have a very similar cps locus, they differ in a single-nucleotide substitution at nucleotide position 483 of the cpsK gene. Similarly, serotypes 1 and 14 have a very similar cps locus but also differ in the same nucleotide substitution of the cpsK gene. Fortunately, this cpsK 483G→C/T substitution can be detected by BstNI restriction endonuclease. A PCR-restriction fragment length polymorphism (RFLP) detection method amplifying a fragment of the cpsK gene digested by BstNI restriction endonuclease was developed and tested in reference strains of these serotypes and also in field isolates.

• Klíčová slova
• BstNI, Streptococcus suis, cpsK, diagnostics,
• MeSH
• polymerázová řetězová reakce MeSH
• polymorfismus délky restrikčních fragmentů MeSH
• prasata MeSH
• séroskupina MeSH
• sérotypizace MeSH
• Streptococcus suis * genetika   MeSH
• streptokokové infekce * diagnóza   veterinární   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Five hundred and fifty-five strains of S. agalactiae of human or bovine origin were serologically typed. In human strains, serotype Ia was the most frequent irrespective of the source and kind of cultivation material, but serotype R was very frequent in urine. In bovine strains, one serotype was found as a rule in one stable both in small private and large socialist farms. The reason for such uniformity of serotypes is not known. Monocolonisation is one of the alternatives, but it seems more reasonable to assume that, the most resistant or more invasive strain will predominate in the herd in the course of time.

• MeSH
• epitopy MeSH
• lidé MeSH
• mastitida skotu mikrobiologie   MeSH
• mléko mikrobiologie   MeSH
• moč mikrobiologie   MeSH
• sérotypizace MeSH
• skot mikrobiologie   MeSH
• Streptococcus agalactiae klasifikace   MeSH
• streptokokové infekce mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot mikrobiologie   MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Československo MeSH
• Názvy látek
• epitopy MeSH

Paired or tripled serum specimens examined for cross reactions between poliomyelitis viruses and other non-polio enteroviruses were obtained from 356 patients (217 males and 139 females) with various clinical diagnoses (aseptic meningitis, meningoencephalitis, paretic neuro-infections, upper respiratory tract infections, bronchopneumonia) who were sampled in 1984 and 1985. The sera were examined by the microneutralization assay technique in flat-bottomed 96-well polyvinyl chloride plates against 100-300 TCD50 of these enterovirus serotypes: CA9, CB1-CB5, E1, E2, E4-E9, E17, E20, E24, E30, EV71 and polioviruses P1-P3. The tests were carried out on BGM or RD cells. Confirmed significant seroconversions to at least one enterovirus serotype were observed in 87 (24.2%) patients. Of these, 27 (31%) reacted by parallel significant or insignificant increases in the heterotypic antibody titre to poliovirus, which accounted for 7.6% of all patients examined. Heterotypic antibody responses may become source of errors in individuals examined for poliovaccine efficacy or the state of specific immunity response to polio, but are unlikely to discredit the overall outcomes of the seroepidemiological surveys conducted in the general population.

• MeSH
• antigeny virové imunologie   MeSH
• Enterovirus klasifikace   imunologie   MeSH
• lidé MeSH
• neutralizační testy MeSH
• Poliovirus klasifikace   imunologie   MeSH
• protilátky virové analýza   MeSH
• sérotypizace MeSH
• zkřížené reakce MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• antigeny virové MeSH
• protilátky virové MeSH

The direct double antibody sandwich (DAS) type of enzyme-linked immunosorbent assay (ELISA) was used to determine the degree of serological and antigenic differences, among the three serotypes (A, B, C) of red clover necrotic mosaic virus (RCNMV). Homologous and heterologous antibody titres in the used IgGs to isolates TpM34 (serotype A), TpM48 (serotype B) and isolate No. 6 (serotype C) as determined by ELISA were 100- to 200-fold higher than by ring precipitation test. Intensity of homologous and heterologous reactions in ELISA depended on the concentration of antigen, of the IgG used for coating and of the labelled IgG. The IgG preparations used contained 50 to 100 times higher concentration of homologous (serotype-specific) than heterologous (interserotype-specific) antibodies. Such a great difference between the two antibody types accounts for a comparatively high degree of selectivity of the homologous reactions.

• MeSH
• antigeny virové analýza   MeSH
• ELISA metody   MeSH
• epitopy MeSH
• imunoglobulin G MeSH
• protilátky virové MeSH
• sérotypizace metody   MeSH
• viry mozaiky klasifikace   imunologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny virové MeSH
• epitopy MeSH
• imunoglobulin G MeSH
• protilátky virové MeSH

The location of type polysaccharides on the cells of reference strains of group-B streptococci of serotypes IV and V and new type candidates NT6 and 7271 was investigated by electronmicroscopy of the bacteria after incubation with homologous type-specific antiserum. A distinct capsular layer was found on the surface of the cells of all these strains. Sialic acid, an integral part of all the conventional type polysaccharides of group-B streptococci, was also detected in all the strains examined.

• MeSH
• bakteriální polysacharidy analýza   imunologie   MeSH
• elektronová mikroskopie MeSH
• imunologické techniky MeSH
• kyseliny sialové analýza   MeSH
• sérotypizace MeSH
• Streptococcus agalactiae analýza   klasifikace   imunologie   ultrastruktura   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální polysacharidy MeSH
• kyseliny sialové MeSH

• Klíčová slova
• ANTIGENS *, LEPTOSPIRA/immunology *,
• MeSH
• antigeny * MeSH
• Leptospira imunologie   MeSH
• lidé MeSH
• séroskupina * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny * MeSH

A total of 73 strains of Plesiomonas shigelloides isolated from humans (24 strains) animals (21 strains) and aquatic environment (28 strains) were determined for their O:H serotype and susceptibility to 18 anti-microbial substances and to the vibriostatic agent O/129. Of all strains, 86.3% were typeable by the O and 94.5% by the H anti-sera used. The serotype distribution was heterogeneous within a country and between the countries. Of the 57 different serotypes identified, O11:H2 (2 strains), O22:H3 (4 strains), O35:HH11 (2 strains), O52:H3 (2 strains) and O90:H6 (2 strains) were found among isolates from humans and animals (mainly in cats) in Finland and Cuba, and O23:H1a1b (3 strains) among isolates from environmental sources in Slovak Republic and Italy. Most (93-100%) of all strains were susceptible to all anti-microbials tested but resistant (92-96%) to the broad-spectrum penicillins (ampicillin, mezlocillin). No correlation between anti-microbial resistance patterns and serotypes was found.

• MeSH
• antibakteriální látky farmakologie   MeSH
• gramnegativní bakteriální infekce mikrobiologie   MeSH
• kočky MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• mikrobiologie vody * MeSH
• nemoci koček mikrobiologie   MeSH
• O-antigeny izolace a purifikace   MeSH
• Plesiomonas klasifikace   účinky léků   růst a vývoj   MeSH
• sérotypizace MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Finsko MeSH
• Kuba MeSH
• Názvy látek
• antibakteriální látky MeSH
• O-antigeny MeSH