AIM: To develop novel DNA extraction and typing procedure for DNA identification of the 7th century human remains, determine the familiar relationship between the individuals, estimate the Y-chromosome haplogroup, and compare the Y-chromosome haplotype with the contemporary populations. METHODS: DNA from preserved femur samples was extracted using the modified silica-based extraction technique. Polymerase chain reaction amplification was performed using human identification kits MiniFiler, Identifiler, and Y-filer and also laboratory-developed and validated Y-chromosome short tandem repeat (STR) pentaplexes with short amplicons. RESULTS: For 244A, 244B, 244C samples, full autosomal DNA profiles (15 STR markers and Amelogenin) and for 244D, 244E, 244F samples, MiniFiler profiles were produced. Y-chromosome haplotypes consisting of up to 24 STR markers were determined and used to predict the Y-chromosome haplogroups and compare the resulting haplotypes with the current population. Samples 244A, 244B, 244C, and 244D belong to Y-chromosome haplogroup R1b and the samples 244E and 244F to haplogroup G2a. Comparison of ancient haplotypes with the current population yielded numerous close matches with genetic distance below 2. CONCLUSION: Application of forensic genetics in archaeology enables retrieving new types of information and helps in data interpretation. The number of successfully typed autosomal and Y-STR loci from ancient specimens in this study is one of the largest published so far for aged samples.

Forensic DNA Service Janovskeho 18 170 00 Prague 7 Czech Republic

000      

02841naa 2200373 a 4500

001      

bmc11019670

003      

CZ-PrNML

005      

20221005142444.0

008      

110714s2009 ci e eng||

009      

AR

024    7_

$a 10.3325/cmj.2009.50.286 $2 doi

035    __

$a (PubMed)19480023

040    __

$a ABA008 $b cze $c ABA008 $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a ci

100    1_

$a Vaněk, Daniel, $d 1965- $7 xx0096434

245    10

$a Kinship and Y-chromosome analysis of 7th century human remains: novel DNA extraction and typing procedure for ancient material / $c D. Vaněk, L. Sasková, H. Koch

314    __

$a Forensic DNA Service, Janovskeho 18, 170 00 Prague 7, Czech Republic. daniel.vanek@DNA.com.cz

520    9_

$a AIM: To develop novel DNA extraction and typing procedure for DNA identification of the 7th century human remains, determine the familiar relationship between the individuals, estimate the Y-chromosome haplogroup, and compare the Y-chromosome haplotype with the contemporary populations. METHODS: DNA from preserved femur samples was extracted using the modified silica-based extraction technique. Polymerase chain reaction amplification was performed using human identification kits MiniFiler, Identifiler, and Y-filer and also laboratory-developed and validated Y-chromosome short tandem repeat (STR) pentaplexes with short amplicons. RESULTS: For 244A, 244B, 244C samples, full autosomal DNA profiles (15 STR markers and Amelogenin) and for 244D, 244E, 244F samples, MiniFiler profiles were produced. Y-chromosome haplotypes consisting of up to 24 STR markers were determined and used to predict the Y-chromosome haplogroups and compare the resulting haplotypes with the current population. Samples 244A, 244B, 244C, and 244D belong to Y-chromosome haplogroup R1b and the samples 244E and 244F to haplogroup G2a. Comparison of ancient haplotypes with the current population yielded numerous close matches with genetic distance below 2. CONCLUSION: Application of forensic genetics in archaeology enables retrieving new types of information and helps in data interpretation. The number of successfully typed autosomal and Y-STR loci from ancient specimens in this study is one of the largest published so far for aged samples.

590    __

$a bohemika - dle Pubmed

650    _2

$a DNA fingerprinting $x metody $7 D016172

650    _2

$a soudní antropologie $7 D018732

650    _2

$a soudní genetika $7 D053803

650    _2

$a zkameněliny $7 D005580

650    _2

$a geny vázané na chromozom Y $7 D050173

650    _2

$a genetické markery $7 D005819

650    _2

$a genotyp $7 D005838

650    _2

$a dějiny starověku $7 D049690

650    _2

$a lidé $7 D006801

650    _2

$a mužské pohlaví $7 D008297

650    _2

$a mikrosatelitní repetice $7 D018895

650    _2

$a polymerázová řetězová reakce $7 D016133

655    _2

$a historické články $7 D016456

655    _2

$a práce podpořená grantem $7 D013485

700    1_

$a Sasková-Přenosilová, Lenka $7 xx0112006

700    1_

$a Koch, Hubert

773    0_

$t Croatian Medical Journal $w MED00173528 $g Roč. 50, č. 3 (2009), s. 286-295

856    41

$u https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2702742/ $y plný text volně přístupný

910    __

$a ABA008 $b x $y 2 $z 0

990    __

$a 20110715115104 $b ABA008

991    __

$a 20221005142439 $b ABA008

999    __

$a ok $b bmc $g 864503 $s 729548

BAS    __

$a 3

BMC    __

$a 2009 $x MED00173528 $b 50 $c 3 $d 286-295 $m Croatian medical journal $n Croatian Medical Journal

LZP    __

$a 2011-3B09/Bjvme