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The course of infection of Encephalitozoon cuniculi genotype I in mice possess combination of features reported in genotypes II and III
B. Sak, K. Brdíčková, N. Holubová, D. Květoňová, L. Hlásková, M. Kváč
Language English Country United States
Document type Journal Article
- MeSH
- Albendazole administration & dosage MeSH
- CD4 Antigens genetics MeSH
- CD8 Antigens genetics MeSH
- Anti-Infective Agents administration & dosage MeSH
- Encephalitozoon cuniculi classification genetics MeSH
- Encephalitozoonosis immunology parasitology MeSH
- Genotype MeSH
- Immunocompetence MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Mice, Inbred BALB C MeSH
- Mice, Inbred C57BL MeSH
- Mice, Knockout MeSH
- Mice, SCID MeSH
- Mice MeSH
- Polymerase Chain Reaction MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Out of three genotypes of Encephalitozoon cuniculi (I-III) available for experimental studies, E. cuniculi genotype I remains the less characterized. This study describes for the first time individual phases of microsporidiosis caused by E. cuniculi genotype I and efficacy of albendazole treatment in immunocompetent BALB/c and C57Bl/6 mice and immunodeficient SCID, CD4-/- and CD8-/- mice using molecular detection and quantification methods. We demonstrate asymptomatic infection despite an intense dissemination of microsporidia into most organs within the first weeks post infection, followed by a chronic infection characterized by significant microsporidia persistence in immunocompetent, CD4-/- and CD8-/- mice and a lethal outcome for SCID mice. Albendazole application led to loss E. cuniculi genotype I infection in immunocompetent mouse strains, decreased spore burden by half in CD4-/- and CD8-/- mice, and prolongation of survival of SCID mice. These results showed Encephalitozoon cuniculi genotype I infection extend and albendazole sensitivity was comparable to E. cuniculi genotype II, but the infection onset speed and mortality rate was similar to E. cuniculi genotype III. These imply that differences in the course of infection and the response to treatment depend not only on immunological status of the host, but also on the genotype causing the infection.
Faculty of Science University of South Bohemia in České Budějovice České Budějovice Czech Republic
Institute of Parasitology Biology Centre Czech Academy of Science České Budějovice Czech Republic
References provided by Crossref.org
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