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Smear layer deproteinization with NaOCl and HOCl: Do application/wash-out times affect dentin bonding of one-step self-etch adhesives

K. Sanon, T. Hatayama, A. Tichy, O. Thanatvarakorn, T. Prasansuttiporn, T. Wada, M. Ikeda, K. Hosaka, M. Nakajima

. 2022 ; 41 (3) : 353-362. [pub] 20220125

Language English Country Japan

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22018285

This study investigated the influence of application/wash-out times of sodium hypochlorite (NaOCl) and hypochlorous acid (HOCl) on dentin bond strength of one-step self-etch adhesives (1-SEAs). Human coronal dentin discs with a standardized smear layer were pretreated with 6% NaOCl or 100 ppm HOCl for 5 s, 15 s, or 30 s, and washed out with water for 5 s, 15 s or 30 s with or without the application of Clearfil DC Activator (CDA). No pretreatment was used as a control. The discs were bonded with a 1-SEA (Bond Force II or Clearfil Universal Bond Quick) and microtensile bond strength (μTBS) was measured after 24 h. Pretreatment with NaOCl for 15 s and 30 s significantly decreased μTBS (p<0.05), irrespective of wash-out time. The application of CDA recovered μTBS but did not outperform the control group. Conversely, pretreatment with HOCl for 15 s and 30 s followed by 30 s wash-out time significantly increased μTBS of 1-SEAs (p<0.05), regardless of CDA application.

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$a Sanon, Kittisak $u Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University $u Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University
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$a Hatayama, Takashi $u Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
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$a Wada, Takahiro $u Department of Advanced Biomaterials, Graduate School of Medical and Dental Sciences, Medical and Dental Science and Technology, Tokyo Medical and Dental University
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