OBJECTIVES: To evaluate the effect of smear layer deproteinization using hypochlorous acid (HOCl) with/without metal chlorides (SrCl2 and ZnCl2) on the microtensile bond strength (μTBS) of two simplified adhesives to dentin. METHODS: Human dentin surfaces with a standardized smear layer were pretreated using a 105 ppm HOCl solution with/without SrCl2 (0.05 M, 0.1 M, 0.2 M, 0.4 M) or ZnCl2 (0.05 M, 0.1 M, 0.2 M) for 5 s, 15 s, or 30 s. After the deproteinizing solution was washed out with water for 5 s, 15 s, or 30 s, pretreated surfaces were bonded with one-step self-etch adhesive Bond Force II or universal adhesive Clearfil Universal Bond Quick, and μTBS was measured after 24 h. Additionally, the deproteinizing effects of HOCl solutions with/without the metal chlorides were compared by measuring changes in the amide:phosphate ratio using attenuated total reflection Fourier transform infrared spectroscopy. Statistical analysis was performed using multifactor ANOVA, Tukey's post hoc tests and t-tests (p < 0.05). RESULTS: Pretreatment with pure HOCl for 15 s and 30 s significantly decreased the amide:phosphate ratio (p < 0.05), indicating effective deproteinization, but the μTBS of both adhesives increased significantly only if HOCl was washed out for 30 s (p < 0.05). Increasing the concentrations of metal chlorides enabled shortening of the wash-out time down to 5 s while maintaining the improved μTBS (p < 0.05). The deproteinizing effect of HOCl was not significantly altered by the addition of metal chlorides (p > 0.05). SIGNIFICANCE: The effectiveness of smear layer deproteinization using HOCl can be improved by the addition of metal chlorides, as their increasing concentration allowed to shorten the wash-out time from 30 s down to 5 s.
- MeSH
- amidy analýza farmakologie MeSH
- chloridy analýza farmakologie MeSH
- dentin chemie MeSH
- dentinová adheziva chemie MeSH
- fosfáty farmakologie MeSH
- kyselina chlorná analýza farmakologie MeSH
- lidé MeSH
- pevnost v tahu MeSH
- pryskyřičné cementy chemie MeSH
- smear layer * MeSH
- testování materiálů MeSH
- vazba zubní * MeSH
- zubní cementy farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
This study investigated the influence of application/wash-out times of sodium hypochlorite (NaOCl) and hypochlorous acid (HOCl) on dentin bond strength of one-step self-etch adhesives (1-SEAs). Human coronal dentin discs with a standardized smear layer were pretreated with 6% NaOCl or 100 ppm HOCl for 5 s, 15 s, or 30 s, and washed out with water for 5 s, 15 s or 30 s with or without the application of Clearfil DC Activator (CDA). No pretreatment was used as a control. The discs were bonded with a 1-SEA (Bond Force II or Clearfil Universal Bond Quick) and microtensile bond strength (μTBS) was measured after 24 h. Pretreatment with NaOCl for 15 s and 30 s significantly decreased μTBS (p<0.05), irrespective of wash-out time. The application of CDA recovered μTBS but did not outperform the control group. Conversely, pretreatment with HOCl for 15 s and 30 s followed by 30 s wash-out time significantly increased μTBS of 1-SEAs (p<0.05), regardless of CDA application.
The effect of smear layer-deproteinizing pretreatment using hypochlorous acid (HOCl) on the micro-shear bond strengths (μSBS) of conventional and resin-modified glass-ionomer cements (GIC) to dentin was investigated and compared with demineralizing pretreatment with polyacrylic acid (PAA). Three GICs: Fuji IX GP Extra (restorative conventional GIC), GC Fuji II LC EM (restorative resin-modified GIC), and GC Fuji Luting EX (luting resin-modified GIC), were used. One hundred fifty human molars were divided into groups (n = 10) according to the cements and dentin pretreatments; no pretreatment (control), 10 s PAA pretreatment, and HOCl pretreatment for 5, 15, or 30 s. After 24 h, μSBS was tested and the data were statistically analyzed using a two-way ANOVA, followed by Tukey's post-hoc test. HOCl pretreatment significantly increased μSBS of conventional GIC compared to the control group. For resin-modified restorative GIC, 5 s HOCl deproteinization significantly increased μSBS, while longer application times did not. There was no significant difference between HOCl-pretreated and control groups of resin-modified luting GIC. PAA pretreatment increased the μSBS of all cements significantly. In conclusion, smear layer deproteinization with HOCl can enhance the dentin bonding of conventional GIC. However, the residual radicals may adversely affect the polymerization of resin-modified GICs.
OBJECTIVES: To evaluate the sealing performance of self-etch adhesives (SEA) after smear layer deproteinization with chemo-mechanical caries removal agents (CRA) to enamel and dentin cavity walls in comparison with 6% NaOCl smear layer deproteinizing. METHODS: Eighty extracted bovine incisors with tapered cylindrical cavities (2.5 mm upper diameter, 1.5 mm lower diameter, 2 mm depth) at the cemento-enamel junction were randomly divided into four pre-treatment groups; no treatment (control), Papacarie (papain enzyme-based CRA), Carisolv (NaOCl-based CRA), and 6% NaOCl. After pretreatment, G-Bond Plus (GBP, one-step SEA) or Clearfil SE Bond 2 (CSE, two-step SEA) was applied to the pre-treated cavity, and then filled with a flowable resin composite (Clearfil Majesty ES Flow). Gap formations at the enamel and dentin lateral walls, and on the dentin cavity floor were investigated using swept-source optical coherence tomography (SS-OCT) after 24-h water storage at 37 °C. Deproteinizing effects of the tested agents on smear layer-covered enamel and dentin were quantified by changes in the amide:phosphate ratio using ATR-FTIR analysis. RESULTS: Papacarie significantly reduced gap formation with both self-etch adhesives at all locations, compared to the control group (p < 0.05). In contrast, increased gap formation was observed with Carisolv and NaOCl. In most groups, CSE exhibited significantly lower gap formation than GBP (p < 0.05). ATR-FTIR revealed a significant decrease in the amide:phosphate ratio for all the deproteinizing agents (p < 0.05). CONCLUSIONS: Chemo-mechanical CRAs could effectively remove the organic phase of enamel and dentin smear layer. The papain enzyme-based gel Papacarie could improve the sealing performance of the self-etch adhesives to both enamel and dentin.
- MeSH
- adheziva MeSH
- dentin MeSH
- dentinová adheziva MeSH
- pryskyřičné cementy MeSH
- skot MeSH
- složené pryskyřice MeSH
- smear layer * MeSH
- testování materiálů MeSH
- vazba zubní * MeSH
- zubní cementy MeSH
- zubní sklovina MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Rozšiřující se indikace pro využití adhezivních systémů ve stomatologii vedou v poslední době k jejich častějšímu používání v zubních ordinacích. Reakcí výrobců na jejich spotřebu je vývoj stále nových produktů a vyznat se mezi jednotlivými typy není jednoduché. Následující text přináší přehled současných adheziv, upozorňuje na možné chyby při jejich použití a nabízí různá doporučení pro jejich aplikaci v klinické praxi.
Práce hodnotí stupeň čistoty stěn kořenového kanálku v závislosti na způsobu výplachu. Porovnává efekt výplachu obyčejnou injekční stříkačkou se zabroušenou jehlou, kanylou a strojovým zařízením Rinse-Endo (výrobce Dürr Dental, Německo). Experiment byl proveden na 12 extrahovaných jednokořenových zubech, které byly standardním způsobem endodonticky ošetřeny. Výplach kořenového kanálku byl prováděn třemi způsoby. Po skončení endodontického ošetření byly zuby rozštípnuty a stěny kořenových kanálků mikroskopicky vyšetřeny. Stupeň čistoty kořenových kanálků byl měřen třístupňovou škálou. Byla hodnocena koronální, střední a apikální část kořenového kanálku. K statistickému porovnání výsledků bylo použito Fisherova exaktního testu (p=0,05). Stěny kořenových kanálků, kde k výplachu bylo použito zařízení Rinse-Endo, vykazovaly signifikantně vyšší stupeň čistoty v porovnání s ostatními metodami výplachu.
The authors compare the effect of the various methods of the rinse of the root canal. 12 extracted single root teeth were randomly dividend into 3 groups. The endodontic treatment was performed using hand instruments under standard conditions (EDTA gel in combination with 2% NaOCl). In the first group only hand rinsing was preformed, in the second group the special endodontic canila was used ane in the third group the power driven instrument Rinse Endo, DürrDental, Germany) was used. After the preparation the teeth were split longitudinaly and microscopically observed. The clean areas were quantified and the groups were compared statistically with the Fischer exact test (p=0,05). The cleanliness of the root canal walls was significantly better in the group where Rinse Endo was used.
Autorka se v přehledu zabývá preparačními technikami při ošetření zubního kazu s výjimkou rotační preparace. Preparační techniky se rozdělují na mechanické, chemicko-mechanické, kinetické, hydrokinetické. Mechanická preparace zahrnuje preparaci ruční, rotační, sonickou a ultrasonickou. Autorka uvádí výhody a nevýhody jednotlivých preparačních technik a ukazuje případy z vlastního výzkumu i praxe.
The author gives the preview of various preparation techniques except of the rotary preparation. Preparation techniques include mechanical, chemomechanical, kinetic and hydrokinetic preparation. The mechanical preparation techniques involve A.R.T.. rotary, sonic and ultrasonic preparation. The advantages and disadvantages of these techniques are discussed and documented by clinical and experimental results of the author.
- MeSH
- dentální technologie MeSH
- lasery klasifikace využití MeSH
- lidé MeSH
- mikroabraze zubní skloviny metody využití MeSH
- preparace zubní kavity MeSH
- preparace zubu MeSH
- smear layer MeSH
- ultrazvuková terapie metody přístrojové vybavení využití MeSH
- zubní kaz terapie MeSH
- zubní nástroje MeSH
- Check Tag
- lidé MeSH
