Study of cytokinin metabolism using HPLC with radioisotope detection
Language English Country England, Great Britain Media print
Document type Journal Article
PubMed
9608424
DOI
10.1016/s0731-7085(97)00151-9
PII: S0731-7085(97)00151-9
Knihovny.cz E-resources
- MeSH
- Adenine analogs & derivatives metabolism MeSH
- Adenosine analogs & derivatives metabolism MeSH
- Benzyl Compounds MeSH
- Kinetin MeSH
- Triticum metabolism MeSH
- Purines MeSH
- Radiometry MeSH
- Reproducibility of Results MeSH
- Tritium MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Adenine MeSH
- Adenosine MeSH
- benzylaminopurine MeSH Browser
- Benzyl Compounds MeSH
- Kinetin MeSH
- N(6)-benzyladenosine MeSH Browser
- Purines MeSH
- Tritium MeSH
High performance liquid chromatography (HPLC) with an on-line flow-through radioactivity detector was used to monitor the metabolism of cytokinins ([3H]6-benzylaminopurine and [3H]6-benzylaminopurine riboside) after their incorporation into wheat seedlings. The production and conversion of individual metabolites was assayed within a short time interval (0.5-3 h). Extraction recoveries from plant tissue proved to be 85%. The uptake of both cytokinins was very rapid and differences in their metabolism were already perceptible after 30 min. The individual metabolites were identified as adenine (Ade), adenosine (Ado), benzyladenine-9-glucoside (BA-9G), 6-benzyladenine (BA) and benzyladenosine (BAR). The method is very fast, sensitive and very useful for metabolic studies. The detection limit was 40 pg (220 Bq) for BA at the level of 2 ng ml-1.
References provided by Crossref.org
