Activation of macrophages by food antigens: enhancing effect of gluten on nitric oxide and cytokine production
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10733090
DOI
10.1002/jlb.67.3.312
Knihovny.cz E-resources
- MeSH
- Macrophage Activation immunology MeSH
- Transcriptional Activation drug effects MeSH
- Antigens immunology MeSH
- Cytokines biosynthesis immunology metabolism MeSH
- Nitrites metabolism MeSH
- Gliadin chemical synthesis immunology MeSH
- Glutens immunology pharmacology MeSH
- Interferon-gamma immunology MeSH
- Interleukin-10 biosynthesis metabolism MeSH
- Cells, Cultured MeSH
- RNA, Messenger genetics metabolism MeSH
- Mice, Inbred BALB C MeSH
- Mice, Inbred C3H MeSH
- Mice MeSH
- Nitric Oxide metabolism MeSH
- Peptide Fragments chemical synthesis immunology MeSH
- Macrophages, Peritoneal drug effects enzymology immunology metabolism MeSH
- Food * MeSH
- Nitric Oxide Synthase Type II MeSH
- Nitric Oxide Synthase genetics MeSH
- Tumor Necrosis Factor-alpha biosynthesis metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens MeSH
- Cytokines MeSH
- Nitrites MeSH
- Gliadin MeSH
- Glutens MeSH
- Interferon-gamma MeSH
- Interleukin-10 MeSH
- RNA, Messenger MeSH
- Nos2 protein, mouse MeSH Browser
- Nitric Oxide MeSH
- Peptide Fragments MeSH
- Nitric Oxide Synthase Type II MeSH
- Nitric Oxide Synthase MeSH
- Tumor Necrosis Factor-alpha MeSH
Macrophages play an important role in effector mechanisms of various chronic inflammatory diseases. We studied the effect of gluten, the agent inducing celiac disease, and other food antigens on the activation of macrophages. Nitric oxide (NO) and cytokine production were followed as markers of activation, using cultured murine peritoneal macrophages. None of the food antigens tested caused direct inducible nitric oxide synthase (iNOS) activation in macrophages. Unlike other food antigens gluten, gliadin, and their proteolytic fragments significantly enhanced NO production when applied together with interferon-gamma (IFN-gamma), the most efficient being fragments originating from 25- to 45-min peptic digestion. The activation pathway was mediated via direct stimulation of tumor necrosis factor alpha (TNF-alpha) secretion. The NO-enhancing effect was confirmed at the level of iNOS mRNA transcription. In case of sustained local inflammatory reaction connected with increase of IFN-gamma, gluten and its proteolytic fragments may thus elevate NO production. Increased NO level could consequently participate in the development of mucosal lesions in the gut of celiac patients.
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