PCR assay for detection and differentiation of K88ab(1), K88ab(2), K88ac, and K88ad fimbrial adhesins in E. coli strains isolated from diarrheic piglets
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16110913
DOI
10.1007/bf02931457
Knihovny.cz E-resources
- MeSH
- Antigens, Bacterial analysis classification genetics MeSH
- DNA Primers MeSH
- Escherichia coli classification genetics isolation & purification MeSH
- Genetic Variation MeSH
- Escherichia coli Infections microbiology veterinary MeSH
- Swine Diseases microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Swine MeSH
- Fimbriae Proteins analysis classification genetics MeSH
- Escherichia coli Proteins analysis classification genetics MeSH
- Diarrhea microbiology veterinary MeSH
- DNA Restriction Enzymes metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Slovakia MeSH
- Names of Substances
- Antigens, Bacterial MeSH
- DNA Primers MeSH
- K88 antigen, E coli MeSH Browser
- Fimbriae Proteins MeSH
- Escherichia coli Proteins MeSH
- DNA Restriction Enzymes MeSH
Primers were designed and prepared and conditions were determined for PCR detection and differentiation of enterotoxigenic E. coli bacterial strains isolated from diarrheic pigs. Primers K88/1 and K88/2 are 25 bp oligomers that correspond to a region of genes encoding one of serological variants of the K88 antigen (K88ab(1), K88ab(2), K88ac or K88ad). A positive result of PCR is an amplificate of 792 bp in size for K88ab and K88ad variant or 786 bp for K88ac variant. The individual serological variants of genes of the K88 antigen could be differentiated by cutting the obtained PCR amplificates by restriction endonucleases. The PCR analysis of 674 E. coli strains isolated from diarrheic pigs showed that 184 strains were K88 positive. By using restriction endonucleases the K88-positive strains were in 4 cases classified as K88ab variant, 180 as K88ac variant and none contained gene for the K88ad variant. Ninety-five % coincidence with serological examination using K88ab, K88ac and K88ad specific antibodies was shown.
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J Clin Microbiol. 1988 May;26(5):879-84 PubMed
Zentralbl Veterinarmed B. 1994 Mar;41(1):49-59 PubMed
Vet Res. 1999 Mar-Jun;30(2-3):259-84 PubMed
J Clin Microbiol. 1988 Jan;26(1):149-50 PubMed
Vet Rec. 1993 Oct 30;133(18):439-42 PubMed
Folia Microbiol (Praha). 2002;47(1):73-7 PubMed
Vet Microbiol. 1999 Aug 31;68(3-4):209-17 PubMed
Comp Immunol Microbiol Infect Dis. 1992 Oct;15(4):285-92 PubMed
J Clin Microbiol. 1991 Apr;29(4):745-52 PubMed
Mol Microbiol. 1992 Jan;6(2):247-55 PubMed
Vet Microbiol. 1999 Jul 1;67(4):307-10 PubMed
Microb Pathog. 1986 Feb;1(1):57-69 PubMed
Acta Vet Hung. 1995;43(1):95-103 PubMed
Infect Immun. 1979 Mar;23(3):700-5 PubMed
Infect Immun. 1985 Oct;50(1):279-83 PubMed
Folia Microbiol (Praha). 2003;48(2):277-9 PubMed
Folia Microbiol (Praha). 1999;44(6):729-34 PubMed
Am J Vet Res. 1986 Feb;47(2):213-7 PubMed
Anim Health Res Rev. 2001 Dec;2(2):129-40 PubMed
J Vet Diagn Invest. 1996 Oct;8(4):460-3 PubMed
Acta Pathol Microbiol Scand. 1964;62:439-47 PubMed
Folia Microbiol (Praha). 1994;39(3):171-5 PubMed
Folia Microbiol (Praha). 2004;49(6):751-6 PubMed
Vet Microbiol. 1991 Nov;29(3-4):299-307 PubMed
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