Inference of the phylogenetic position of oxymonads based on nine genes: support for metamonada and excavata
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16120804
DOI
10.1093/molbev/msi245
PII: msi245
Knihovny.cz E-resources
- MeSH
- Peptide Elongation Factor 1 genetics MeSH
- Peptide Elongation Factor 2 genetics MeSH
- Eukaryotic Cells * MeSH
- Flagella MeSH
- Phylogeny * MeSH
- Genes, rRNA MeSH
- Classification MeSH
- Evolution, Molecular * MeSH
- Polymerase Chain Reaction MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Tubulin genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Peptide Elongation Factor 1 MeSH
- Peptide Elongation Factor 2 MeSH
- Tubulin MeSH
Circumscribing major eukaryote groups and resolving higher order relationships between them are among the most challenging tasks facing molecular evolutionists. Recently, evidence suggesting a new supergroup (the Excavata) comprising a wide array of flagellates has been collected. This group consists of diplomonads, retortamonads, Carpediemonas, heteroloboseans, Trimastix, jakobids, and Malawimonas, all of which possess a particular type of ventral feeding groove that is proposed to be homologous. Euglenozoans, parabasalids, and oxymonads have also been associated with Excavata as their relationships to one or more core excavate taxa were demonstrated. However, the main barrier to the general acceptance of Excavata is that its existence is founded primarily on cytoskeletal similarities, without consistent support from molecular phylogenetics. In gene trees, Excavata are typically not recovered together. In this paper, we present an analysis of the phylogenetic position of oxymonads (genus Monocercomonoides) based on concatenation of eight protein sequences (alpha-tubulin, beta-tubulin, gamma-tubulin, EF-1alpha, EF-2, cytosolic (cyt) HSP70, HSP90, and ubiquitin) and 18S rRNA. We demonstrate that the genes are in conflict regarding the position of oxymonads. Concatenation of alpha- and beta-tubulin placed oxymonads in the plant-chromist part of the tree, while the concatenation of other genes recovered a well-supported group of Metamonada (oxymonads, diplomonads, and parabasalids) that branched weakly with euglenozoans--connecting all four excavates included in the analyses and thus providing conditional support for the existence of Excavata.
References provided by Crossref.org
High quality genome assembly of the amitochondriate eukaryote Monocercomonoides exilis
Arginine deiminase pathway enzymes: evolutionary history in metamonads and other eukaryotes
Flavodiiron protein from Trichomonas vaginalis hydrogenosomes: the terminal oxygen reductase
