Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16365848
DOI
10.1002/bip.20433
Knihovny.cz E-resources
- MeSH
- Biosensing Techniques instrumentation methods MeSH
- Nucleic Acid Hybridization methods MeSH
- Nanotechnology instrumentation methods MeSH
- Oligonucleotide Probes chemistry genetics MeSH
- Surface Plasmon Resonance methods MeSH
- Oligonucleotide Array Sequence Analysis instrumentation methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Oligonucleotide Probes MeSH
We have optimized surface plasmon resonance (SPR) biosensor technology for a rapid, direct, and low-consumption label-free multianalyte screening of synthetic oligonucleotides (ONs) with modified internucleotide linkages potentially applicable in antisense therapy. Monitoring of the ONs hybridization is based on the formation of complex between the natural oligonucleotide probe immobilized on the sensor surface and the ON in solution in contact with the sensor surface. An immobilization chemistry utilizing the streptavidin-biotin interaction was employed to obtain desired ligand density and high hybridization efficiency. It was demonstrated that the sensor is capable of detecting complementary 23-mer ONs in concentrations as low as 0.1 nM with high specificity and reproducibility.
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