Small-scale photobioreactors for cultivation of photoautotrophic microbes are required for precise characterization of the growth parameters of wild-type and engineered strains of these organisms, for their screening, and for optimization of culture conditions. Here, we describe the design and use of a flat-cuvette photobioreactor that allows accurate control of culture irradiance, temperature, pH, and gas composition combined with real-time monitoring by a built-in fluorometer and densitometer. The high-power LED light source generates precise irradiance levels that are programmed by user-designed protocols. The irradiance, temperature, and gas composition may be static or dynamically modulated, while optical density and pH may be stabilized in turbidostat and pH-stat modes, respectively. We demonstrate that the instrument is able to detect minute variations of growth caused, for example, by sudden dilution or by circadian rhythms. The sensitivity of the instrument is sufficient to monitor suspension optical density as low as 10(-2). This newly designed photobioreactor can significantly contribute to the study and use of photoautotrophic microbes in systems biology and biotechnology.

Institute of Systems Biology and Ecology ASCR Zámek 136 CZ 37333 Nové Hrady Czech Republic

Citace poskytuje Crossref.org

Novel technique for the ultra-sensitive detection of hazardous contaminants using an innovative sensor integrated with a bioreactor

Marine microalgae Schizochytrium demonstrates strong production of essential fatty acids in various cultivation conditions, advancing dietary self-sufficiency

The balance between photosynthesis and respiration explains the niche differentiation between Crocosphaera and Cyanothece

Plasticity of Cyanobacterial Thylakoid Microdomains Under Variable Light Conditions

Quantifying Oxygen Management and Temperature and Light Dependencies of Nitrogen Fixation by Crocosphaera watsonii

Towards a quantitative assessment of inorganic carbon cycling in photosynthetic microorganisms

Quantitative insights into the cyanobacterial cell economy

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Determination of Storage (Starch/Glycogen) and Total Saccharides Content in Algae and Cyanobacteria by a Phenol-Sulfuric Acid Method

Phenotypic characterization of Synechocystis sp. PCC 6803 substrains reveals differences in sensitivity to abiotic stress

A method to decompose spectral changes in Synechocystis PCC 6803 during light-induced state transitions

Optimizing cyanobacterial product synthesis: Meeting the challenges

Mechanisms of High Temperature Resistance of Synechocystis sp. PCC 6803: An Impact of Histidine Kinase 34

Ultradian metabolic rhythm in the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142

Experimental validation of a nonequilibrium model of CO₂ fluxes between gas, liquid medium, and algae in a flat-panel photobioreactor