Type I restriction-modification enzymes act as conventional adenine methylases on hemimethylated DNAs, but unmethylated recognition targets induce them to translocate thousands of base pairs before cleaving distant sites nonspecifically. The first crystal structure of a type I motor subunit responsible for translocation and cleavage suggests how the pentameric translocating complex is assembled and provides a structural framework for translocation of duplex DNA by RecA-like ATPase motors.

Department of Structure and Function of Proteins Institute of Systems Biology and Ecology Academy of Sciences of the Czech Republic Nove Hrady Czech Republic

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Nucleic Acids Res. 2007;35(7):2227-37 PubMed

J Mol Biol. 1998 Nov 13;283(5):963-76 PubMed

EMBO J. 2006 May 17;25(10):2230-9 PubMed

Microbiol Mol Biol Rev. 2000 Jun;64(2):412-34 PubMed

Nucleic Acids Res. 2006;34(15):4160-7 PubMed

Nucleic Acids Res. 2008 Apr;36(6):1881-90 PubMed

Biol Chem. 1998 Apr-May;379(4-5):497-503 PubMed

Cell. 2006 Dec 29;127(7):1349-60 PubMed

EMBO J. 2008 May 7;27(9):1388-98 PubMed

Cell. 2005 May 6;121(3):363-73 PubMed

Nat Struct Mol Biol. 2004 Sep;11(9):838-43 PubMed

Nucleic Acids Res. 2004 Dec 14;32(22):6540-7 PubMed

EMBO J. 1996 Nov 15;15(22):6335-47 PubMed

J Mol Biol. 1999 Jul 9;290(2):565-79 PubMed

Cell. 2006 Apr 21;125(2):287-300 PubMed

Mol Microbiol. 2004 Jan;51(1):135-47 PubMed

Crystal structure of a novel domain of the motor subunit of the Type I restriction enzyme EcoR124 involved in complex assembly and DNA binding

A residue of motif III positions the helicase domains of motor subunit HsdR in restriction-modification enzyme EcoR124I

The helical domain of the EcoR124I motor subunit participates in ATPase activity and dsDNA translocation

pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I

Functional coupling of duplex translocation to DNA cleavage in a type I restriction enzyme

Interdomain communication in the endonuclease/motor subunit of type I restriction-modification enzyme EcoR124I

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PDB
2W00