Aluminium ions inhibit the formation of diacylglycerol generated by phosphatidylcholine-hydrolysing phospholipase C in tobacco cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20629955
DOI
10.1111/j.1469-8137.2010.03349.x
PII: NPH3349
Knihovny.cz E-resources
- MeSH
- Cell Membrane drug effects metabolism MeSH
- Time Factors MeSH
- Diglycerides biosynthesis MeSH
- Phosphatidylcholines metabolism MeSH
- Type C Phospholipases metabolism MeSH
- Aluminum toxicity MeSH
- Ions MeSH
- Porphobilinogen analogs & derivatives metabolism MeSH
- Pollen Tube growth & development metabolism MeSH
- Nicotiana cytology drug effects enzymology MeSH
- Cell Survival drug effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-diacylglycerol MeSH Browser
- 3-anilino difluoroboron dipyrromethene MeSH Browser
- Diglycerides MeSH
- Phosphatidylcholines MeSH
- Type C Phospholipases MeSH
- Aluminum MeSH
- Ions MeSH
- phosphatidylcholine-specific phospholipase C MeSH Browser
- Porphobilinogen MeSH
• Aluminium ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. This study aimed to assess the impact of Al on the activity of phosphatidylcholine-hydrolysing phospholipase C (PC-PLC), a new member of the plant phospholipase family. • We labelled the tobacco cell line BY-2 and pollen tubes with a fluorescent derivative of phosphatidylcholine and assayed for patterns of fluorescently labelled products. Growth of pollen tubes was analysed. • We observed a significant decrease of labelled diacylglycerol (DAG) in cells treated with AlCl(3). Investigation of possible metabolic pathways that control DAG generation and consumption during the response to Al showed that DAG originated from the reaction catalysed by PC-PLC. The growth of pollen tubes was retarded in the presence of Al and this effect was accompanied by the decrease of labelled DAG similar to the case of the BY-2 cell line. The growth of pollen tubes arrested by Al was rescued by externally added DAG. • Our observation strongly supports the role of DAG generated by PC-PLC in the response of tobacco cells to Al.
References provided by Crossref.org
Phospholipase Dα1 Acts as a Negative Regulator of High Mg2+-Induced Leaf Senescence in Arabidopsis
Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana
