Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
20688378
DOI
10.1016/j.theriogenology.2010.06.005
PII: S0093-691X(10)00309-2
Knihovny.cz E-zdroje
- MeSH
- buněčná membrána fyziologie ultrastruktura MeSH
- centrifugace - gradient hustoty veterinární MeSH
- kapři * MeSH
- kryoprezervace veterinární MeSH
- motilita spermií * MeSH
- oxid křemičitý chemie MeSH
- povidon chemie MeSH
- spermie fyziologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- oxid křemičitý MeSH
- Percoll MeSH Prohlížeč
- povidon MeSH
We attempted to select a fraction of common carp, Cyprinus carpio spermatozoa that best survived a conventional freeze/thaw procedure, by centrifugation of frozen/thawed sperm through a Percoll gradient (45% and 90%). The proportion of motile spermatozoa (65.81 ± 5.19%), their velocity (77.58 ± 31.07 μm/sec), and membrane integrity (83.66 ± 4.38% intact) were significantly higher in separated sperm than in whole samples (motility 23.36 ± 2.98%, velocity 55.55 ± 19.03 μm/sec, and membrane integrity 57.92 ± 4.65%). Our results demonstrated that Percoll gradient centrifugation shows promise as a technique for selecting high quality cryopreserved fish spermatozoa, which could be useful for cryobiological research. Further studies are needed to evaluate the potentially higher fertilizing ability of the separated spermatozoa.
Citace poskytuje Crossref.org
Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp Cyprinus carpio
