This review deals with techniques and methods used in the study of the function and development of microorganisms occurring in soil with emphasis on the contributions of Czech Academician Ivan Málek and his coworkers or fellows (Jiří Macura, František Kunc) to the development of basic techniques used in soil microbiology. Early studies, including batch cultivation and respirometric techniques, as well as later developments of percolation and continuous-flow methods of cultivation of soil microorganisms are discussed. Recent developments in the application of analytical chemistry (HPLC or GC) and of molecular biological techniques to ecological questions that have revolutionized concepts in soil microbiology and microbial ecology are also briefly mentioned, including denaturing gradient gel electrophoresis (DGGE), temperature gradient gel electrophoresis (TGGE), phospholipid fatty acid analysis (PLFA) and others. The shift of soil microbiology from the study of individual microorganisms to entire microbial communities, including nonculturable species, is briefly discussed.

Institute of Microbiology ASCR 5 V 1 Vídeňská 1083 14220 Prague 4 Krč Czech Republic

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Appl Environ Microbiol. 1996 Sep;62(9):3112-20 PubMed

Folia Microbiol (Praha). 1969;14(4):327-33 PubMed

Appl Microbiol Biotechnol. 2002 Oct;60(1-2):33-44 PubMed

Int Microbiol. 2005 Sep;8(3):195-204 PubMed

Can J Microbiol. 1958 Jun;4(3):303-16 PubMed

J Gen Microbiol. 1973 May;76(1):85-99 PubMed

Microbiol Rev. 1981 Jun;45(2):211-43 PubMed

Folia Microbiol (Praha). 1993;38(5):376-8 PubMed

Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1970;124(3):245-9 PubMed

Folia Microbiol (Praha). 1992;37(2):128-32 PubMed

Nature. 1958 Dec 27;182(4652):1796-7 PubMed

Folia Microbiol (Praha). 1965 Mar;10:115-24 PubMed

Folia Microbiol (Praha). 1962 Nov;7:388-411 PubMed

Annu Rev Microbiol. 1979;33:169-99 PubMed

Appl Environ Microbiol. 1998 Feb;64(2):613-7 PubMed

J Gen Microbiol. 1975 Nov;91(1):99-109 PubMed

Biochem J. 1946;40(5-6):803-15 PubMed

Folia Microbiol (Praha). 1992;37(2):122-7 PubMed

Anal Biochem. 1960 Dec 30;1:511-6 PubMed

Nature. 1953 Mar 14;171(4350):481 PubMed

J Appl Bacteriol. 1969 Jun;32(2):259-60 PubMed

Plant Physiol. 1952 Jan;27(1):70-80 PubMed

Microbiol Mol Biol Rev. 2002 Sep;66(3):506-77, table of contents PubMed

Nature. 1953 Jul 4;172(4366):29-30 PubMed

Folia Microbiol (Praha). 1965 Sep;10(5):302-23 PubMed

Anal Biochem. 1962 Jun;3:464-71 PubMed

Anal Biochem. 1962 Jun;3:439-47 PubMed

Can J Microbiol. 1960 Aug;6:439-52 PubMed

J Bacteriol. 1958 Aug;76(2):207-16 PubMed

Bioresour Technol. 2010 Feb;101(4):1348-54 PubMed

J Biol Chem. 1962 Mar;237:929-35 PubMed

Can J Microbiol. 1970 May;16(5):299-304 PubMed

Appl Environ Microbiol. 1997 Nov;63(11):4516-22 PubMed

FEMS Microbiol Rev. 2006 Mar;30(2):215-42 PubMed

Folia Microbiol (Praha). 1968;13(1):46-96 PubMed

J Microbiol Methods. 1984;2:165-76 PubMed

Anal Biochem. 1962 Nov;4:413-7 PubMed

Microbiol Mol Biol Rev. 1998 Sep;62(3):597-635 PubMed

Appl Microbiol. 1961 Mar;9(2):150-6 PubMed

Appl Microbiol. 1968 Feb;16(2):232-5 PubMed

Folia Microbiol (Praha). 1999;44(4):377-9 PubMed

Appl Environ Microbiol. 1993 Mar;59(3):695-700 PubMed

Res Microbiol. 2007 Jun;158(5):447-55 PubMed

Appl Environ Microbiol. 2009 Jun;75(11):3611-20 PubMed

FEMS Microbiol Rev. 2005 Jan;29(1):3-23 PubMed

Folia Microbiol (Praha). 1965 Jan;10:36-43 PubMed

Folia Microbiol (Praha). 1966;11(6):479-535 PubMed

Acta Microbiol Pol. 1983;32(3):257-63 PubMed

Appl Environ Microbiol. 1976 Jul;32(1):188-9 PubMed

Folia Microbiol (Praha). 1970;15(2):129-51 PubMed

FEMS Microbiol Rev. 2008 May;32(3):501-21 PubMed