SCIMP, a transmembrane adaptor protein involved in major histocompatibility complex class II signaling
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21930792
PubMed Central
PMC3209250
DOI
10.1128/mcb.05817-11
PII: MCB.05817-11
Knihovny.cz E-zdroje
- MeSH
- adaptorové proteiny signální transdukční chemie metabolismus MeSH
- aktivace lymfocytů MeSH
- antigen prezentující buňky imunologie MeSH
- B-lymfocyty imunologie metabolismus MeSH
- C-terminální Src kinasa MeSH
- fosfoproteiny metabolismus MeSH
- HEK293 buňky MeSH
- histokompatibilita - antigeny třídy II imunologie metabolismus MeSH
- imunologické synapse chemie MeSH
- lidé MeSH
- malá interferující RNA MeSH
- membránové mikrodomény chemie metabolismus MeSH
- membránové proteiny chemie genetika imunologie metabolismus MeSH
- mitogenem aktivované proteinkinasy metabolismus MeSH
- molekulární sekvence - údaje MeSH
- prezentace antigenu MeSH
- RNA interference MeSH
- sekvence aminokyselin MeSH
- signální transdukce MeSH
- skupina kinas odvozených od src-genu metabolismus MeSH
- src homologní domény MeSH
- T-lymfocyty imunologie MeSH
- tyrosinkinasy chemie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adaptorové proteiny signální transdukční MeSH
- B cell linker protein MeSH Prohlížeč
- C-terminální Src kinasa MeSH
- CSK protein, human MeSH Prohlížeč
- fosfoproteiny MeSH
- histokompatibilita - antigeny třídy II MeSH
- lyn protein-tyrosine kinase MeSH Prohlížeč
- malá interferující RNA MeSH
- membránové proteiny MeSH
- mitogenem aktivované proteinkinasy MeSH
- SCIMP protein, human MeSH Prohlížeč
- skupina kinas odvozených od src-genu MeSH
- SLP-76 signal Transducing adaptor proteins MeSH Prohlížeč
- tyrosinkinasy MeSH
Formation of the immunological synapse between an antigen-presenting cell (APC) and a T cell leads to signal generation in both cells involved. In T cells, the lipid raft-associated transmembrane adaptor protein LAT plays a central role. Its phosphorylation is a crucial step in signal propagation, including the calcium response and mitogen-activated protein kinase activation, and largely depends on its association with the SLP76 adaptor protein. Here we report the discovery of a new palmitoylated transmembrane adaptor protein, termed SCIMP. SCIMP is expressed in B cells and other professional APCs and is localized in the immunological synapse due to its association with tetraspanin-enriched microdomains. In B cells, it is constitutively associated with Lyn kinase and becomes tyrosine phosphorylated after major histocompatibility complex type II (MHC-II) stimulation. When phosphorylated, SCIMP binds to the SLP65 adaptor protein and also to the inhibitory kinase Csk. While the association with SLP65 initiates the downstream signaling cascades, Csk binding functions as a negative regulatory loop. The results suggest that SCIMP is involved in signal transduction after MHC-II stimulation and therefore serves as a regulator of antigen presentation and other APC functions.
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Al-Daccak R., Mooney N., Charron D. 2004. MHC class II signaling in antigen-presenting cells. Curr. Opin. Immunol. 16:108–113 PubMed
Anderson H. A., Hiltbold E. M., Roche P. A. 2000. Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation. Nat. Immunol. 1:156–162 PubMed
Angelisova P., Hilgert I., Horejsi V. 1994. Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 39:249–256 PubMed
Berditchevski F., et al. 2001. Analysis of the CD151-alpha3beta1 integrin and CD151-tetraspanin interactions by mutagenesis. J. Biol. Chem. 276:41165–41174 PubMed
Bobbitt K. R., Justement L. B. 2000. Regulation of MHC class II signal transduction by the B cell coreceptors CD19 and CD22. J. Immunol. 165:5588–5596 PubMed
Brdicka T., et al. 2000. Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation. J. Exp. Med. 191:1591–1604 PubMed PMC
Brdickova N., et al. 2003. LIME: a new membrane raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J. Exp. Med. 198:1453–1462 PubMed PMC
Brown D. A. 2006. Lipid rafts, detergent-resistant membranes, and raft targeting signals. Physiology (Bethesda) 21:430–439 PubMed
Brown K., Levitt D., Shannon M., Link B. 2001. Phase II trial of Remitogen (humanized 1D10) monoclonal antibody targeting class II in patients with relapsed low-grade or follicular lymphoma. Clin. Lymphoma 2:188–190 PubMed
Cole P. A., Shen K., Qiao Y., Wang D. 2003. Protein tyrosine kinases Src and Csk: a tail's tale. Curr. Opin. Chem. Biol. 7:580–585 PubMed
Crotty S. 2011. Follicular helper CD4 T cells (TFH). Annu. Rev. Immunol. 29:621–663 PubMed
de Castro E., et al. 2006. ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins. Nucleic Acids Res. 34:W362–W365 PubMed PMC
Engels N., Wollscheid B., Wienands J. 2001. Association of SLP-65/BLNK with the B cell antigen receptor through a non-ITAM tyrosine of Ig-[alpha]. Eur. J. Immunol. 31:2126–2134 PubMed
Fooksman D. R., et al. 2010. Functional anatomy of T cell activation and synapse formation. Annu. Rev. Immunol. 28:79–105 PubMed PMC
Fu C., Turck C. W., Kurosaki T., Chan A. C. 1998. BLNK: a central linker protein in B cell activation. Immunity 9:93–103 PubMed
Fuller D. M., Zhang W. 2009. Regulation of lymphocyte development and activation by the LAT family of adapter proteins. Immunol. Rev. 232:72–83 PubMed PMC
Geng L., Raab M., Rudd C. E. 1999. Cutting edge: SLP-76 cooperativity with FYB/FYN-T in the up-regulation of TCR-driven IL-2 transcription requires SLP-76 binding to FYB at Tyr595 and Tyr651. J. Immunol. 163:5753–5757 PubMed
Grabbe A., Wienands J. 2006. Human SLP-65 isoforms contribute differently to activation and apoptosis of B lymphocytes. Blood 108:3761–3768 PubMed
Gram H., Schmitz R., Zuber J. F., Baumann G. 1997. Identification of phosphopeptide ligands for the Src-homology 2 (SH2) domain of Grb2 by phage display. Eur. J. Biochem. 246:633–637 PubMed
Haylett R. S., Koch N., Rink L. 2009. MHC class II molecules activate NFAT and the ERK group of MAPK through distinct signaling pathways in B cells. Eur. J. Immunol. 39:1947–1955 PubMed
Horejsi V., Otahal P., Brdicka T. 2010. LAT—an important raft-associated transmembrane adaptor protein. FEBS J. 277:4383–4397 PubMed
Hur E. M., et al. 2003. LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation. J. Exp. Med. 198:1463–1473 PubMed PMC
Charrin S., et al. 2009. Lateral organization of membrane proteins: tetraspanins spin their web. Biochem. J. 420:133–154 PubMed
Jackman J. K., et al. 1995. Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells. J. Biol. Chem. 270:7029–7032 PubMed
Janssen E., Zhu M., Zhang W., Koonpaew S. 2003. LAB: a new membrane-associated adaptor molecule in B cell activation. Nat. Immunol. 4:117–123 PubMed
Jin L., et al. 2008. MPYS, a novel membrane tetraspanner, is associated with major histocompatibility complex class II and mediates transduction of apoptotic signals. Mol. Cell. Biol. 28:5014–5026 PubMed PMC
Kawabuchi M., et al. 2000. Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases. Nature 404:999–1003 PubMed
Koretzky G. A., Abtahian F., Silverman M. A. 2006. SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat. Rev. Immunol. 6:67–78 PubMed
Krogh A., Larsson B., von Heijne G., Sonnhammer E. L. L. 2001. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305:567–580 PubMed
Kropshofer H., et al. 2002. Tetraspan microdomains distinct from lipid rafts enrich select peptide-MHC class II complexes. Nat. Immunol. 3:61–68 PubMed
Lang P., et al. 2001. TCR-induced transmembrane signaling by peptide/MHC class II via associated Ig-alpha/beta dimers. Science 291:1537–1540 PubMed
Li S. S. 2005. Specificity and versatility of SH3 and other proline-recognition domains: structural basis and implications for cellular signal transduction. Biochem. J. 390:641–653 PubMed PMC
Lingwood D., Kaiser H. J., Levental I., Simons K. 2009. Lipid rafts as functional heterogeneity in cell membranes. Biochem. Soc. Trans. 37:955–960 PubMed
Mittelbrunn M., Yanez-Mo M., Sancho D., Ursa A., Sanchez-Madrid F. 2002. Cutting edge: dynamic redistribution of tetraspanin CD81 at the central zone of the immune synapse in both T lymphocytes and APC. J. Immunol. 169:6691–6695 PubMed
Muller K., et al. 1996. Rapid identification of phosphopeptide ligands for SH2 domains. Screening of peptide libraries by fluorescence-activated bead sorting. J. Biol. Chem. 271:16500–16505 PubMed
Nadolski M. J., Linder M. E. 2007. Protein lipidation. FEBS J. 274:5202–5210 PubMed
Nagy Z. A., et al. 2002. Fully human, HLA-DR-specific monoclonal antibodies efficiently induce programmed death of malignant lymphoid cells. Nat. Med. 8:801–807 PubMed
Patterson H. C., Kraus M., Kim Y. M., Ploegh H., Rajewsky K. 2006. The B cell receptor promotes B cell activation and proliferation through a non-ITAM tyrosine in the Igalpha cytoplasmic domain. Immunity 25:55–65 PubMed
Resh M. D. 2006. Palmitoylation of ligands, receptors, and intracellular signaling molecules. Sci. STKE 2006:re14. PubMed
Riol-Blanco L., et al. 2009. Immunological synapse formation inhibits, via NF-[kappa]B and FOXO1, the apoptosis of dendritic cells. Nat. Immunol. 10:753–760 PubMed
Rodriguez-Pinto D. 2005. B cells as antigen presenting cells. Cell Immunol. 238:67–75 PubMed
Rubinstein E., et al. 1996. CD9, CD63, CD81, and CD82 are components of a surface tetraspan network connected to HLA-DR and VLA integrins. Eur. J. Immunol. 26:2657–2665 PubMed
Sala-Valdes M., et al. 2006. EWI-2 and EWI-F link the tetraspanin web to the actin cytoskeleton through their direct association with ezrin-radixin-moesin proteins. J. Biol. Chem. 281:19665–19675 PubMed
Sauer K., et al. 2001. Hematopoietic progenitor kinase 1 associates physically and functionally with the adaptor proteins B cell linker protein and SLP-76 in lymphocytes. J. Biol. Chem. 276:45207–45216 PubMed
Savina A., Amigorena S. 2007. Phagocytosis and antigen presentation in dendritic cells. Immunol. Rev. 219:143–156 PubMed
Sonnhammer E. L., von Heijne G., Krogh A. 1998. A hidden Markov model for predicting transmembrane helices in protein sequences. Proc. Int. Conf. Intell. Syst. Mol. Biol. 6:175–182 PubMed
Unternaehrer J. J., Chow A., Pypaert M., Inaba K., Mellman I. 2007. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc. Natl. Acad. Sci. U. S. A. 104:234–239 PubMed PMC
Wan J., Roth A. F., Bailey A. O., Davis N. G. 2007. Palmitoylated proteins: purification and identification. Nat. Protoc. 2:1573–1584 PubMed
Yanez-Mo M., Barreiro O., Gordon-Alonso M., Sala-Valdes M., Sanchez-Madrid F. 2009. Tetraspanin-enriched microdomains: a functional unit in cell plasma membranes. Trends Cell Biol. 19:434–446 PubMed
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