The effect of adenosine on pro-inflammatory cytokine production by porcine T cells
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22222199
DOI
10.1016/j.vetimm.2011.12.002
PII: S0165-2427(11)00470-3
Knihovny.cz E-resources
- MeSH
- Adenosine-5'-(N-ethylcarboxamide) pharmacology MeSH
- Adenosine agonists physiology MeSH
- CD3 Complex physiology MeSH
- CD4-Positive T-Lymphocytes drug effects physiology MeSH
- CD8-Positive T-Lymphocytes drug effects physiology MeSH
- Quinazolines pharmacology MeSH
- Interferon-gamma biosynthesis physiology MeSH
- Interleukin-10 biosynthesis physiology MeSH
- Interleukin-2 biosynthesis physiology MeSH
- Concanavalin A pharmacology MeSH
- Swine immunology MeSH
- Flow Cytometry veterinary MeSH
- T-Lymphocyte Subsets drug effects physiology MeSH
- T-Lymphocytes drug effects physiology MeSH
- Tumor Necrosis Factor-alpha biosynthesis physiology MeSH
- Triazoles pharmacology MeSH
- Dose-Response Relationship, Drug MeSH
- Inflammation physiopathology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 9-chloro-2-(2-furyl)-(1,2,4)triazolo(1,5-c)quinazolin-5-imine MeSH Browser
- Adenosine-5'-(N-ethylcarboxamide) MeSH
- Adenosine MeSH
- CD3 Complex MeSH
- Quinazolines MeSH
- Interferon-gamma MeSH
- Interleukin-10 MeSH
- Interleukin-2 MeSH
- Concanavalin A MeSH
- Tumor Necrosis Factor-alpha MeSH
- Triazoles MeSH
Adenosine is a well described anti-inflammatory modulator of immune responses. The aim of the present study was to describe the role of common adenosine agonist 5'-N-ethylcarboxamidoadenosine (NECA) in cytokine production by main porcine T cell subpopulations. TNF-α, IFN-γ, IL-2 and IL-10 were detected by multicolor flow cytometry together with cell surface markers CD3, CD4 and CD8. It was found that NECA inhibits (in a dose-dependent manner) production of pro-inflammatory TNF-α and Th1-associated cytokines IFN-γ, IL-2 in all concanavalin A-stimulated T cell subpopulations. Moreover, production of IL-10 was potentiated in all T cell subpopulations tested. These corresponded well with the fact that all T cell subsets expressed mRNA for adenosine receptor (AR) subtypes to comparable extents. Contrary to concanavalin A-stimulated cells, NECA had a moderate effect on PMA-stimulated T cells, suggesting that AR in pigs acts via signaling pathways not associated with protein-kinase C. Non-selective antagonist CGS15943 as well as allosteric modulator SCH202676 failed to reverse the effect of NECA in pigs. In conclusion, NECA has an anti-inflammatory effect on porcine T cell subpopulations.
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