Paramagnetic particles coupled with an automated flow injection analysis as a tool for influenza viral protein detection
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biosensing Techniques instrumentation methods MeSH
- Biotin chemistry metabolism MeSH
- Electrochemical Techniques instrumentation methods MeSH
- Electrodes MeSH
- Hemagglutinin Glycoproteins, Influenza Virus analysis metabolism MeSH
- Cadmium analysis MeSH
- Quantum Dots MeSH
- Limit of Detection MeSH
- Linear Models MeSH
- Magnetite Nanoparticles chemistry MeSH
- Flow Injection Analysis instrumentation methods MeSH
- Mercury chemistry MeSH
- Cadmium Compounds chemistry MeSH
- Streptavidin chemistry metabolism MeSH
- Sulfides chemistry MeSH
- Carbon chemistry MeSH
- Influenza A Virus, H5N1 Subtype chemistry isolation & purification MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biotin MeSH
- cadmium sulfide MeSH Browser
- Hemagglutinin Glycoproteins, Influenza Virus MeSH
- Cadmium MeSH
- Magnetite Nanoparticles MeSH
- Mercury MeSH
- Cadmium Compounds MeSH
- Streptavidin MeSH
- Sulfides MeSH
- Carbon MeSH
Currently, the influenza virus infects millions of individuals every year. Since the influenza virus represents one of the greatest threats, it is necessary to develop a diagnostic technique that can quickly, inexpensively, and accurately detect the virus to effectively treat and control seasonal and pandemic strains. This study presents an alternative to current detection methods. The flow-injection analysis-based biosensor, which can rapidly and economically analyze a wide panel of influenza virus strains by using paramagnetic particles modified with glycan, can selectively bind to specific viral A/H5N1/Vietnam/1203/2004 protein-labeled quantum dots. Optimized detection of cadmium sulfide quantum dots (CdS QDs)-protein complexes connected to paramagnetic microbeads was performed using differential pulse voltammetry on the surface of a hanging mercury drop electrode (HMDE) and/or glassy carbon electrode (GCE). Detection limit (3 S/N) estimations based on cadmium(II) ions quantification were 0.1 μg/mL or 10 μg/mL viral protein at HMDE or GCE, respectively. Viral protein detection was directly determined using differential pulse voltammetry Brdicka reaction. The limit detection (3 S/N) of viral protein was estimated as 0.1 μg/mL. Streptavidin-modified paramagnetic particles were mixed with biotinylated selective glycan to modify their surfaces. Under optimized conditions (250 μg/mL of glycan, 30-min long interaction with viral protein, 25°C and 400 rpm), the viral protein labeled with quantum dots was selectively isolated and its cadmium(II) content was determined. Cadmium was present in detectable amounts of 10 ng per mg of protein. Using this method, submicrogram concentrations of viral proteins can be identified.
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