Structure-function relationships during transgenic telomerase expression in Arabidopsis
Jazyk angličtina Země Dánsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23278240
DOI
10.1111/ppl.12021
Knihovny.cz E-zdroje
- MeSH
- Arabidopsis enzymologie genetika MeSH
- buněčné jadérko enzymologie genetika MeSH
- buněčné jádro enzymologie genetika MeSH
- geneticky modifikované rostliny MeSH
- jaderné lokalizační signály genetika MeSH
- katalytická doména genetika MeSH
- listy rostlin genetika MeSH
- proteiny huseníčku genetika metabolismus MeSH
- proteosyntéza MeSH
- regulace genové exprese u rostlin MeSH
- sestřih RNA MeSH
- tabák genetika MeSH
- telomerasa chemie genetika metabolismus MeSH
- terciární struktura proteinů MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- jaderné lokalizační signály MeSH
- proteiny huseníčku MeSH
- telomerasa MeSH
Although telomerase (EC 2.7.7.49) is important for genome stability and totipotency of plant cells, the principles of its regulation are not well understood. Therefore, we studied subcellular localization and function of the full-length and truncated variants of the catalytic subunit of Arabidopsis thaliana telomerase, AtTERT, in planta. Our results show that multiple sites in AtTERT may serve as nuclear localization signals, as all the studied individual domains of the AtTERT were targeted to the nucleus and/or the nucleolus. Although the introduced genomic or cDNA AtTERT transgenes display expression at transcript and protein levels, they are not able to fully complement the lack of telomerase functions in tert -/- mutants. The failure to reconstitute telomerase function in planta suggests a more complex telomerase regulation in plant cells than would be expected based on results of similar experiments in mammalian model systems.
Citace poskytuje Crossref.org
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