Structure-function relationships during transgenic telomerase expression in Arabidopsis
Language English Country Denmark Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23278240
DOI
10.1111/ppl.12021
Knihovny.cz E-resources
- MeSH
- Arabidopsis enzymology genetics MeSH
- Cell Nucleolus enzymology genetics MeSH
- Cell Nucleus enzymology genetics MeSH
- Plants, Genetically Modified MeSH
- Nuclear Localization Signals genetics MeSH
- Catalytic Domain genetics MeSH
- Plant Leaves genetics MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Protein Biosynthesis MeSH
- Gene Expression Regulation, Plant MeSH
- RNA Splicing MeSH
- Nicotiana genetics MeSH
- Telomerase chemistry genetics metabolism MeSH
- Protein Structure, Tertiary MeSH
- Structure-Activity Relationship MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nuclear Localization Signals MeSH
- Arabidopsis Proteins MeSH
- Telomerase MeSH
Although telomerase (EC 2.7.7.49) is important for genome stability and totipotency of plant cells, the principles of its regulation are not well understood. Therefore, we studied subcellular localization and function of the full-length and truncated variants of the catalytic subunit of Arabidopsis thaliana telomerase, AtTERT, in planta. Our results show that multiple sites in AtTERT may serve as nuclear localization signals, as all the studied individual domains of the AtTERT were targeted to the nucleus and/or the nucleolus. Although the introduced genomic or cDNA AtTERT transgenes display expression at transcript and protein levels, they are not able to fully complement the lack of telomerase functions in tert -/- mutants. The failure to reconstitute telomerase function in planta suggests a more complex telomerase regulation in plant cells than would be expected based on results of similar experiments in mammalian model systems.
References provided by Crossref.org
Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex
Telomerase Interaction Partners-Insight from Plants
Composition and Function of Telomerase-A Polymerase Associated with the Origin of Eukaryotes
Telomeres in Plants and Humans: Not So Different, Not So Similar
An armadillo-domain protein participates in a telomerase interaction network
Telomere- and Telomerase-Associated Proteins and Their Functions in the Plant Cell
Telomere dynamics in the lower plant Physcomitrella patens
