Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Cupriavidus necator růst a vývoj metabolismus MeSH
- dusík metabolismus MeSH
- hydrolýza MeSH
- hydroxybutyráty metabolismus MeSH
- kultivační média chemie MeSH
- mléčné bílkoviny metabolismus MeSH
- polyestery metabolismus MeSH
- subtilisiny metabolismus MeSH
- syrovátkové proteiny MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dusík MeSH
- hydroxybutyráty MeSH
- kultivační média MeSH
- mléčné bílkoviny MeSH
- poly-beta-hydroxybutyrate MeSH Prohlížeč
- polyestery MeSH
- subtilisiny MeSH
- syrovátkové proteiny MeSH
Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. Addition of WWH (10 % (v/v) of cultivation media) supported the growth and PHB accumulation; PHB yields in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivations. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated with WWH produced 28.1 g PHB l(-1) resulting in a very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were ~40 % higher than in the control cultivation, WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.
Citace poskytuje Crossref.org
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