Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cupriavidus necator growth & development metabolism MeSH
- Nitrogen metabolism MeSH
- Hydrolysis MeSH
- Hydroxybutyrates metabolism MeSH
- Culture Media chemistry MeSH
- Milk Proteins metabolism MeSH
- Polyesters metabolism MeSH
- Subtilisins metabolism MeSH
- Whey Proteins MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nitrogen MeSH
- Hydroxybutyrates MeSH
- Culture Media MeSH
- Milk Proteins MeSH
- poly-beta-hydroxybutyrate MeSH Browser
- Polyesters MeSH
- Subtilisins MeSH
- Whey Proteins MeSH
Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. Addition of WWH (10 % (v/v) of cultivation media) supported the growth and PHB accumulation; PHB yields in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivations. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated with WWH produced 28.1 g PHB l(-1) resulting in a very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were ~40 % higher than in the control cultivation, WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.
References provided by Crossref.org
Novel thermophilic polyhydroxyalkanoates producing strain Aneurinibacillus thermoaerophilus CCM 8960
